Core B: Cell Culture and Molecular Technology

核心 B：细胞培养和分子技术

• 批准号: 8733036
• 负责人: Kevin D Cooper
• 金额: $ 16.95万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8733036
• 关键词: Archives; B-Lymphocytes; Basic Science; Bioinformatics; Biological Assay; Biology; Biopsy; Cell Culture Techniques; Cell Line; Cells; Clinic; Collaborations; Communities; Computer software; Consultations; Core Facility; Cost Savings; Cultured Cells; Cutaneous; Data; Data Analyses; Dermal; Dermatologic; Development; Diagnostic; Disease; Doctor of Medicine; Doctor of Philosophy; Education; Emerging Technologies; Epidermis; Epigenetic Process; Experimental Designs; Faculty; Fibroblasts; Fostering; Foundations; Funding; Gene Delivery; Gene Expression; Genome; Genomics; Genotype; Goals; Hair follicle structure; Human Resources; Institution; Knowledge; Lasers; Leadership; Learning; Maintenance; Measures; Methodology; Methylation; Microscopy; Mission; Molecular; Molecular Profiling; National Institute of Arthritis and Musculoskeletal and Skin Diseases; Paraffin Embedding; Pathway Analysis; Pathway interactions; Patient Care; Plant Roots; Play; Proteins; Proteomics; Protocols documentation; Quality Control; RNA; Recruitment Activity; Research; Research Activity; Research Personnel; Research Project Grants; Resources; Role; Sampling; Science; Services; Skin; Small Interfering RNA; Source; Speed; Systems Biology; Techniques; Technology; Time; Tissues; Training; Translational Research; Translations; Work; base; career development; cell type; cost effective; data acquisition; deep sequencing; design; experience; human data; improved; innovation; interdisciplinary collaboration; keratinocyte; knowledge base; medical schools; melanocyte; member; new technology; open source; research study; skin disorder; tool; transcriptomics; vector

The SDRC Cell Culture and Molecular Technology Core (CCMTC) provides established, well characterized skin cell lines and primary cultures, as well as services and training in systems biology approaches to the study of skin cells. The core continuously evolves depending on investigator needs and provides state-of-the- art services, training, facilities and expertise. The CCMTC has developed into a major hub of research activity on campus and has an active role in introducing new investigators to work on cutaneous disease. The core is heavily engaged in providing a wide range of cultured skin cells to investigators, and in providing training in cell culture methodology and emerging molecular and systems biology technology. The three major missions of the CCMTC are listed below. Specific Aim 1: To provide cost-effective service and training in state-of-the-art cell culture and molecular technology to promote skin-related research. List of activities include the provision of cultured epidermal and dermal cells (keratinocytes, melanocytes, fibroblasts and immunocytes) to SDRC investigators in an efficient, quality-controlled, and cost effective manner. Provision of, access to and training in a wide range of molecular biologic technologies (e.g., siRNA methodology, retroviral immortalization, vector-based gene delivery, etc.). Specific Aim 2: To provide SDRC investigators with skin-specific 'Omics and systems biology data analysis, consultation, technologies and software to facilitate a "pipeline" of inter-core translational research and interdisciplinary projects involving skin research. The CCMTC serves as a knowledge base for skin-specific transcriptomics and proteomics and provides expertise with the plan, design and bioinformatics analysis of these emerging technologies. Specific Aim 3: To promote career development with a skin-centered emphasis for research residents, fellows and faculty members by helping them to learn state of the art cell culture and molecular techniques as they build a body of preliminary data for research projects. Due to investigator demand, the CCMTC will now offer several new technologies including metabolome analysis from skin cells/tissue, DASL assay for gene expression profiles from paraffin-embedded tissue, and whole genome methylation for epigenetic studies.

SDRC细胞培养和分子技术核心（CCMTC）提供了成熟的，充分表征的 皮肤细胞系和原代培养，以及系统生物学方法的服务和培训， 研究皮肤细胞。核心根据研究者的需求不断发展，并提供最新的 艺术服务、培训、设施和专业知识。CCMTC已发展成为一个主要的研究中心 活动在校园里，并在介绍新的研究人员对皮肤病的工作积极的作用。 核心是大量从事提供广泛的培养皮肤细胞的研究人员，并在提供 细胞培养方法学和新兴分子和系统生物学技术培训。三 以下列出了CCMTC的主要任务。具体目标1：提供具有成本效益的服务和培训 在最先进的细胞培养和分子技术，以促进皮肤相关的研究。活动清单 包括提供培养的表皮和真皮细胞（角质形成细胞、黑素细胞、成纤维细胞 免疫细胞）以高效、质量受控和成本有效的方式提供给SDRC研究者。提供 广泛的分子生物学技术的获取和培训（例如，siRNA方法， 逆转录病毒永生化、基于载体的基因递送等）。具体目标2：为SDRC调查人员提供 与皮肤特定的“组学和系统生物学数据分析，咨询，技术和软件， 促进核心间转化研究和涉及皮肤研究的跨学科项目的“管道”。 CCMTC作为皮肤特异性转录组学和蛋白质组学的知识库， 这些新兴技术的规划、设计和生物信息学分析的专业知识。具体目标3： 促进以皮肤为中心的研究住院医师，研究员和教师的职业发展 通过帮助他们学习最先进的细胞培养和分子技术， 研究项目的初步数据。由于研究者的需求，CCMTC现在将提供几个新的 技术包括皮肤细胞/组织的代谢组分析、基因表达谱的DASL测定 从石蜡包埋的组织，和全基因组甲基化用于表观遗传学研究。