Molecular mechanisms of action of macrolide antibiotics

大环内酯类抗生素的分子作用机制

基本信息

  • 批准号:
    8640960
  • 负责人:
  • 金额:
    $ 30.31万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2013
  • 资助国家:
    美国
  • 起止时间:
    2013-04-01 至 2017-01-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Macrolide antibiotics inhibit cell growth by interfering with protein synthesis. These drugs bind in the nascent peptide exit tunnel and, according to the widely accepted view, inhibit synthesis of all cellular proteins at the early rounds of translation In contrast to this conventional model of macrolide action, our preliminary studies showed that treatment of Gram-positive and Gram-negative bacteria with macrolides allows for continued translation of a defined subset of proteins. Further, the ability of the protein to evade inhibitio is determined by its N-terminal sequence which can bypass the antibiotic in the exit tunnel without displacing the drug from its binding site. After the initial bypass, translation of some proteins cn continue until their completion, whereas synthesis of some polypeptides can be arrested at later stages. Both of these effects depend on the structure of the antibiotic. In spite of the functional and medical significance of these phenomena, the molecular mechanisms underlying the ability of the protein to evade inhibition and the requirements for the drug-induced translation arrest are unknown and will be addressed in this project. Whole-cell proteomics will be used to comprehensively characterize proteins whose translation continues in the presence of the antibiotic. The highly-innovative technique of ribosome profiling will provide genome-wide information of the sites of drug-dependent 'late' translation arrest. The whole cell-studies will b followed by biochemical characterization of molecular mechanisms of bypass and arrest carried out in a cell-free translation system. Finally, the correlation between the structure of the antibiotic bound in the ribosomal exit tunnel and the spectrum of proteins synthesized in antibiotic-treated cells will be analyzed and physiological consequences of the variation in the composition of the resistome will be examined. The anticipated findings should significantly expand the understanding of the general mode of action of clinically-important macrolide antibacterials and open new venues for development of protein synthesis inhibitors with superior antibiotic properties.
性状(由申请方提供):大环内酯类抗生素通过干扰蛋白质合成抑制细胞生长。这些药物结合在新生的肽出口隧道,并根据广泛接受的观点,抑制所有细胞蛋白质的合成在翻译的早期回合相反,这种传统的大环内酯类作用模型,我们的初步研究表明,治疗革兰氏阳性和革兰氏阴性细菌与大环内酯类允许继续翻译的一个定义的蛋白质子集。此外,蛋白逃避抑制的能力由其N-末端序列决定,其可以绕过出口通道中的抗生素而不将药物从其结合位点置换。在最初的旁路之后,一些蛋白质的翻译可以继续直到它们完成,而一些多肽的合成可以在稍后阶段被阻止。这两种作用都取决于抗生素的结构。尽管功能 和医学意义的这些现象,分子机制的蛋白质的能力,以逃避抑制和药物诱导的翻译逮捕的要求是未知的,将在这个项目中解决。全细胞蛋白质组学将用于全面表征在抗生素存在下继续翻译的蛋白质。高度创新的核糖体分析技术将提供药物依赖性“晚期”翻译停滞位点的全基因组信息。全细胞研究将B,然后是在无细胞翻译系统中进行的旁路和阻滞分子机制的生物化学表征。最后,将分析结合在核糖体出口通道中的抗生素的结构与抗生素处理的细胞中合成的蛋白质的谱之间的相关性,并检查耐药基因组的组成的变化的生理后果。预期的研究结果应显着扩大临床重要的大环内酯类抗菌药物的一般作用模式的理解和开放的新场所开发具有上级抗生素性能的蛋白质合成抑制剂。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

ALEXANDER S MANKIN其他文献

ALEXANDER S MANKIN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('ALEXANDER S MANKIN', 18)}}的其他基金

Advancing ribosome-targeting antibacterial peptides with a unique mechanism of action
以独特的作用机制推进核糖体靶向抗菌肽
  • 批准号:
    10443921
  • 财政年份:
    2022
  • 资助金额:
    $ 30.31万
  • 项目类别:
Advancing ribosome-targeting antibacterial peptides with a unique mechanism of action
以独特的作用机制推进核糖体靶向抗菌肽
  • 批准号:
    10569098
  • 财政年份:
    2022
  • 资助金额:
    $ 30.31万
  • 项目类别:
Advancing ribosome-targeting antibacterial peptides with a unique mechanism of action
以独特的作用机制推进核糖体靶向抗菌肽
  • 批准号:
    10436039
  • 财政年份:
    2021
  • 资助金额:
    $ 30.31万
  • 项目类别:
Equipment Supplement Request for Purchasing Amersham Typhoon RGB Phosphorimager (for R35GM127134)
购买 Amersham Typhoon RGB 荧光成像仪(适用于 R35GM127134)的设备补充申请
  • 批准号:
    10386084
  • 财政年份:
    2018
  • 资助金额:
    $ 30.31万
  • 项目类别:
Exploiting antibiotics to understand the ribosome and translation
利用抗生素来了解核糖体和翻译
  • 批准号:
    10366000
  • 财政年份:
    2018
  • 资助金额:
    $ 30.31万
  • 项目类别:
Exploiting antibiotics to understand the ribosome and translation
利用抗生素来了解核糖体和翻译
  • 批准号:
    9897557
  • 财政年份:
    2018
  • 资助金额:
    $ 30.31万
  • 项目类别:
Context-specific action of antibiotics targeting the catalytic center of the bacterial ribosome
针对细菌核糖体催化中心的抗生素的特定作用
  • 批准号:
    9158354
  • 财政年份:
    2016
  • 资助金额:
    $ 30.31万
  • 项目类别:
Context-specific action of antibiotics targeting the catalytic center of the bacterial ribosome
针对细菌核糖体催化中心的抗生素的特定作用
  • 批准号:
    9332339
  • 财政年份:
    2016
  • 资助金额:
    $ 30.31万
  • 项目类别:
Molecular mechanisms of action of macrolide antibiotics
大环内酯类抗生素的分子作用机制
  • 批准号:
    8482422
  • 财政年份:
    2013
  • 资助金额:
    $ 30.31万
  • 项目类别:
Programmed translation arrest controlled by nascent peptides and antibiotics
由新生肽和抗生素控制的程序化翻译停滞
  • 批准号:
    8917273
  • 财政年份:
    2012
  • 资助金额:
    $ 30.31万
  • 项目类别:

相似海外基金

RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
  • 批准号:
    2327346
  • 财政年份:
    2024
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Standard Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
  • 批准号:
    2312555
  • 财政年份:
    2024
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Standard Grant
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
  • 批准号:
    BB/Z514391/1
  • 财政年份:
    2024
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Training Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
  • 批准号:
    ES/Z502595/1
  • 财政年份:
    2024
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
  • 批准号:
    23K24936
  • 财政年份:
    2024
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
  • 批准号:
    ES/Z000149/1
  • 财政年份:
    2024
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
  • 批准号:
    2901648
  • 财政年份:
    2024
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Studentship
ERI: Developing a Trust-supporting Design Framework with Affect for Human-AI Collaboration
ERI:开发一个支持信任的设计框架,影响人类与人工智能的协作
  • 批准号:
    2301846
  • 财政年份:
    2023
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Standard Grant
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
  • 批准号:
    488039
  • 财政年份:
    2023
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Operating Grants
How motor impairments due to neurodegenerative diseases affect masticatory movements
神经退行性疾病引起的运动障碍如何影响咀嚼运动
  • 批准号:
    23K16076
  • 财政年份:
    2023
  • 资助金额:
    $ 30.31万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了