Adipose Tissue Core

脂肪组织核心

• 批准号: 8639523
• 负责人: YIYING ZHANG
• 金额: $ 15.4万
• 依托单位: ST. LUKE'S-ROOSEVELT INST FOR HLTH SCIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2014-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639523
• 关键词: Adipocytes; Adipose tissue; Antibodies; Area; Biogenesis; Biology; Characteristics; Collaborations; Comorbidity; Computer software; Development; Dyslipidemias; Equipment; Experimental Designs; Functional disorder; Funding; Histologic; Human; In Vitro; Individual; Investigation; Investments; Light Microscope; Liver diseases; Maintenance; Measurement; Measures; Metabolic Diseases; New York; Non-Insulin-Dependent Diabetes Mellitus; Nutritional Study; Obesity; Pathologic; Phenotype; Postdoctoral Fellow; Protocols documentation; Research; Research Personnel; Rodent; Services; Staging System; Students; Techniques; Tissues; Training; United States National Institutes of Health; Universities; Validation; base; cost effective; in vivo; insight; interest; member; non-alcoholic fatty liver; nutrition; study characteristics

Excess adipose tissue is the defining characteristic of obesity. The phenotype and function of adipose tissue are closely associated with many metabolic diseases, including type 2 diabetes mellitus, non-alcoholic fatty liver disease and dyslipidemia. Understanding adipose tissue function and biogenesis are critical to gain insights into the development of obesity and its co-morbidities. The New York Obesity Nutrition Research Center (NYONRC) includes about 50 federally-funded investigators. The Adipose Tissue Core (AT Core) provides an efficient and cost-effective means for investigators in our research base to study the characteristics and functions of adipose tissue in normal biology and pathologic states. Rationale for Adipose Tissue Core: The NYONRC has a large cadre of NIH-funded investigators who vary widely in their expertise and areas of investigation. To many of these investigators, characterizing the phenotype and function of adipose tissue is critical for their studies. The AT Core brings together the expertise and equipment to provide comprehensive adipose tissue analysis, which would otherwise be unavailable or significantly more expensive for individual NYONRC investigators. One example is that in the AT Core the sizing of adipocytes through histologic analysis of many sections of adipose tissue employs a Nikon light microscope with an automated stage system, CD camera and software that identifies and measures the size of each individual adipocyte in a field. Another example is that FACS analyses that the AT Core performs for NYONRC investigators requires development and validation of protocols for each combination of fluorescently tagged antibodies and the maintenance of two expensive FACS machines. The equipment required for these services was purchased by Columbia University. Because the NYONRC leverages the investments in equipment made by Columbia University, the AT Core is able to provide services that would otherwise be unavailable to NYONRC investigators or significantly more expensive.

过多的脂肪组织是肥胖的定义特征。脂肪组织的表型和功能 与许多代谢性疾病密切相关，包括2型糖尿病、非酒精性脂肪性糖尿病、 肝病和血脂异常。了解脂肪组织的功能和生物发生对于获得 深入了解肥胖症及其并发症的发展。纽约肥胖营养研究 中心（NYONRC）包括大约50名联邦资助的调查人员。脂肪组织核心（AT核心） 为我们研究基地的研究人员提供一个有效和符合成本效益的方法， 脂肪组织在正常生物学和病理状态下的特性和功能。 脂肪组织芯的原理：NYONRC拥有大量NIH资助的研究人员，他们的专业知识和研究领域差异很大。对于许多研究者来说，脂肪组织的表型和功能的特征对于他们的研究至关重要。AT Core汇集了专业知识和设备，以提供全面的脂肪组织分析，否则NYONRC研究人员将无法获得或花费更高。一个例子是，在AT Core中，通过对脂肪组织的许多切片进行组织学分析来确定脂肪细胞的大小，采用了具有自动载物台系统、CD相机和软件的Nikon光学显微镜，该软件可识别和测量视野中每个单独脂肪细胞的大小。另一个例子是，AT Core为NYONRC调查人员进行的FACS分析需要为荧光标记抗体的每种组合开发和验证方案，并维护两台昂贵的FACS机器。这些服务所需的设备由哥伦比亚大学购买。由于NYONRC利用了哥伦比亚大学对设备的投资，AT Core能够提供NYONRC调查人员无法获得的服务或更昂贵的服务。