Adipose Tissue Core

脂肪组织核心的基本原理：NYONRC有一大批由nih资助的研究人员，他们的专业知识和研究领域各不相同。对许多研究者来说，表征脂肪组织的表型和功能对他们的研究至关重要。AT核心汇集了专业知识和设备，以提供全面的脂肪组织分析，否则将不可用或显着更昂贵的个别NYONRC调查员。一个例子是，在AT Core中，通过对许多脂肪组织切片进行组织学分析来确定脂肪细胞的大小，使用尼康光学显微镜，配有自动分级系统，CD相机和软件，可以识别和测量一个领域中每个单个脂肪细胞的大小。另一个例子是，AT核心为NYONRC研究人员执行的FACS分析需要开发和验证每种荧光标记抗体组合的协议，并维护两台昂贵的FACS机器。这些服务所需的设备由哥伦比亚大学购买。由于NYONRC利用了哥伦比亚大学在设备上的投资，因此AT Core能够提供NYONRC调查人员无法获得的服务，或者提供的服务要昂贵得多。

Rationale for Adipose Tissue Core: The NYONRC has a large cadre of NIH-funded investigators who vary widely in their expertise and areas of investigation. To many of these investigators, characterizing the phenotype and function of adipose tissue is critical for their studies. The AT Core brings together the expertise and equipment to provide comprehensive adipose tissue analysis, which would otherwise be unavailable or significantly more expensive for individual NYONRC investigators. One example is that in the AT Core the sizing of adipocytes through histologic analysis of many sections of adipose tissue employs a Nikon light microscope with an automated stage system, CD camera and software that identifies and measures the size of each individual adipocyte in a field. Another example is that FACS analyses that the AT Core performs for NYONRC investigators requires development and validation of protocols for each combination of fluorescently tagged antibodies and the maintenance of two expensive FACS machines. The equipment required for these services was purchased by Columbia University. Because the NYONRC leverages the investments in equipment made by Columbia University, the AT Core is able to provide services that would otherwise be unavailable to NYONRC investigators or significantly more expensive.