课题基金 / 基金详情

项目摘要

项目成果

Jon P Anderson的其他基金

相似基金

相关文献

中文摘要
翻译
描述(由申请人提供):该产品是涂有二氧化硅胶体晶体的载玻片,用于结合蛋白质电泳和基质辅助激光解吸/电离质谱(MALDI-MS)检测,两者都具有优异的性能。II期计划将开发一种仪器,方便地将载玻片应用于体内糖蛋白的蛋白质组学。该提案是普渡大学的Wirth小组和LI-COR生物科学公司之间的合作,前者开发了用于蛋白质分离的新媒体,可以与MALDI-MS结合,后者的核心竞争力是生物分析仪器的商业化。使用改性二氧化硅胶体晶体表面作为MALDI-MS底物的初步结果表明,加合物形成减少,质量分辨率提高。此外,二氧化硅胶体晶体表面是使用等电聚焦分离蛋白质的介质。结合使用单薄层晶体表面分离和检测蛋白质的能力,实现高分辨率蛋白质分离和改进的MALDI-MS检测,将扩大使用MALDI-MS识别基于翻译后修饰的新型生物标志物的实用性。第一阶段的具体目标是优化二氧化硅晶体性能,表征适用的分子量范围,研究使用该底物的直接结果是分辨率的提高和加合物形成的减少,并将蛋白质分离技术与MALDI-MS检测在单个二氧化硅胶体晶体表面结合起来。
英文摘要
DESCRIPTION (provided by applicant): The product is a slide coated with silica colloidal crystals to combine protein electrophoresis with matrix- assisted laser desorption/ionization mass spectrometry (MALDI-MS) detection, giving superior performance in both. The Phase II proposal will be to develop an instrument for facile application of the slide in proteomics of intat glycoproteins. This proposal is a collaboration between the Wirth group at Purdue, who have developed new media for protein separations that can be coupled with MALDI-MS, and LI-COR, Biosciences, whose core competency is the commercialization of instruments for bioanalysis. Preliminary results using a modified silica colloidal crystalline surface as a MALDI-MS substrate show a reduction in adduct formation, and an increase in mass resolution. Additionally, the silica colloidal crystalline surface is a medium to separate proteins using isoelectric focusing. Combining the ability to both separate and detect proteins using a single thin layer crystalline surface that enables high resolution protein separation and improved MALDI-MS detection will expand the utility of using MALDI-MS for identifying novel biomarkers based on post translational modifications. The phase I specific aims are to optimize the silica crystal propertie, to characterize the applicable molecular weight range, to study the enhanced resolution and the reduction in adduct formation as a direct result of using this substrate, and to combine protein separation techniques with MALDI-MS detection on a single silica colloidal crystalline surface.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Silica Colloidal Crystals for High Resolution MALDI-MS of Glycoproteins
  • 批准号:
    8927044
  • 项目类别:
  • 资助金额:
    $30.65万
  • 财政年份:
    2014
  • 负责人:
    Jon P Anderson
  • 依托单位:
Novel Hydrocyanine Probes for the Accurate Detection of Reactive Oxygen Species
  • 批准号:
    8647969
  • 项目类别:
  • 资助金额:
    $21.29万
  • 财政年份:
    2014
  • 负责人:
    Jon P Anderson
  • 依托单位:
Metal Enhancement for Near-IR Fluorescence Based Membrane Assays
  • 批准号:
    7325405
  • 项目类别:
  • 资助金额:
    $10.0万
  • 财政年份:
    2007
  • 负责人:
    Jon P Anderson
  • 依托单位:
Metal Enhancement for a Near-IR Fluorescence Scanner
  • 批准号:
    6932939
  • 项目类别:
  • 资助金额:
    $20.04万
  • 财政年份:
    2005
  • 负责人:
    Jon P Anderson
  • 依托单位:
海外基金