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Administrative Supplements to Recover Losses Due to Hurricane Sandy

弥补飓风桑迪造成的损失的行政补充

基本信息

批准号：
8742732
负责人：
Margarita Vigodner
金额：
$ 10.35万
依托单位：
YESHIVA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-12-06 至 2015-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8742732
关键词：
Administrative Supplement Adult Affect Amino Acids Aneuploidy Animals Antibodies Area Bioinformatics Biological Process Buffers Cell Culture Techniques Cell Line Cell Separation Cell physiology Cells Cellular biology Co-Immunoprecipitations Complex Congenital Abnormality Contraceptive Agents Couples Cytolysis Data Development Disease Environment Event Failure Foundations Funding Germ Germ Cells Goals Healthcare Hour Human Human Resources Hurricane Immunoprecipitation Individual Infertility Knowledge Laboratories Lead Male Infertility Malignant neoplasm of testis Mass Spectrum Analysis Mediating Meiosis Mitotic Modification Mus Nature New England New York City Pathway interactions Population Procedures Proteins Proteome Proteomics Protocols documentation Publishing Reagent Regulation Reproduction Research Research Personnel Role Sampling Small Ubiquitin-Related Modifier Proteins Somatic Cell Source Spermatids Spermatocytes Spermatogenesis Spermatogenic Cell Spermatogonia Spontaneous abortion Stable Isotope Labeling Staging Testicular Tissue Testing Testis Time Ubiquitin Universities Western Blotting Work base cell type crosslink experience improved inhibitor/antagonist innovation isopeptidase liquid chromatography mass spectrometry male melting novel novel therapeutics repaired research study undergraduate student

项目摘要

The research progress of our lab was seriously affected by the hurricane Sandy that devastated the northeastern United States, especially New York City. On October 29th 2012, lower Manhattan, more specifically the area to the south of 40th Street suffered from an unexpected major power outage that lasted for a week. During this period, the power to the laboratory was completely shut down. All the fridges and freezers in the laboratory remained off during the power outage due to the lack of a backup generator in the building. A -80°C freezer’s compressor was damaged due to the sudden power outage and the freezer had to be sent out for repair afterwards. The major power outage caused by hurricane Sandy caused severe losses to our laboratory materials. Lysates and cells, including costly cell line stocks, were stored in the -80°C freezer. These valuable materials thawed and melted and were therefore damaged. Some of the lysate samples are very difficult to produce and collect. These samples required large amounts of time, work and expense to manufacture. They were generated from a large number of experimental animals including adult and pubertal mice through several rounds of a 10 hour STA-PUT separation procedure and were stored in the freezer for following immunoprecipitations (IP) and mass-spectrometry identifications. Numerous antibodies were required to confirm those IP experiments, all of which were stored in either a -20°C freezer or 4°C fridge. Consequently these antibodies were also spoiled during the power outage. Additionally, all reagents useful for the SUMO project (such as lysis buffer, media, western blot reagents etc.) were stored in the fridges and freezers and were also ruined. Due to the aforementioned losses caused by “Sandy”, more funds will be needed to replace the reagents, antibodies and personnel time.

桑迪飓风严重影响了我们实验室的研究进程，美国东北部，尤指纽约市。2012年10月29日，曼哈顿，更确切地说是40街以南的地区，持续了一周的大停电在此期间，实验室的电力完全关闭。实验室里所有的冰箱和冰柜在整个实验过程中都保持关闭状态。由于大楼里没有备用发电机而停电。-80 ° C冷冻箱由于突然停电，压缩机被损坏，冷冻机不得不被送出事后修复。飓风桑迪造成的大停电造成严重的我们实验室材料的损失。裂解物和细胞，包括昂贵的细胞系储备，储存在-80 ° C冰箱中。这些珍贵的材料融化了，因此，损坏一些裂解物样品很难生产和收集。这些样品需要大量的时间、工作和费用来制造。它们产生于大量的实验动物，包括成年和青春期小鼠，通过几个循环10小时STA-PUT分离程序，并储存在冷冻机中用于以下免疫沉淀（IP）和质谱鉴定。许多抗体被需要确认这些IP实验，所有这些实验都储存在-20 ° C冰箱或 4°C冰箱。因此，这些抗体在停电期间也被破坏。此外，所有用于SUMO项目的试剂（如裂解缓冲液、培养基、蛋白质印迹）试剂等）都被储存在冰箱和冰柜里，也被毁坏了。由于“桑迪”造成的上述损失，试剂、抗体和人员时间。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Identification of cell-specific targets of sumoylation during mouse spermatogenesis.

DOI：
10.1530/rep-15-0239
发表时间：
2016-02
期刊：
Reproduction (Cambridge, England)
影响因子：
0
作者：
Xiao Y;Pollack D;Andrusier M;Levy A;Callaway M;Nieves E;Reddi P;Vigodner M
通讯作者：
Vigodner M

Cross-talk between sumoylation and phosphorylation in mouse spermatocytes.

DOI：
10.1016/j.bbrc.2017.04.107
发表时间：
2017-06-03
期刊：
Biochemical and biophysical research communications
影响因子：
3.1
作者：
Xiao Y;Lucas B;Molcho E;Vigodner M
通讯作者：
Vigodner M

Inhibition of CDK1 activity by sumoylation.

通过sumoylation抑制CDK1活性。

DOI：
10.1016/j.bbrc.2016.08.051
发表时间：
2016-09-16
期刊：
Biochemical and biophysical research communications
影响因子：
3.1
作者：
Xiao Y;Lucas B;Molcho E;Schiff T;Vigodner M
通讯作者：
Vigodner M

Identification of sumoylated targets in proliferating mouse spermatogonia and human testicular seminomas.

DOI：
10.4103/aja.aja_11_20
发表时间：
2020-11
期刊：
Asian journal of andrology
影响因子：
2.9
作者：
Vigodner M;Lucas B;Kemeny S;Schwartz T;Levy R
通讯作者：
Levy R

Can your protein be sumoylated? A quick summary and important tips to study SUMO-modified proteins.

DOI：
10.1016/j.ab.2014.11.006
发表时间：
2015-05-15
期刊：
ANALYTICAL BIOCHEMISTRY
影响因子：
2.9
作者：
Xiao, Yuxuan;Pollack, Daniel;Nieves, Edward;Winchell, Aby;Callaway, Myrasol;Vigodner, Margarita
通讯作者：
Vigodner, Margarita