Administrative Supplements to Recover Losses Due to Hurricane Sandy
Administrative Supplements to Recover Losses Due to Hurricane Sandy
批准号:
8742732
负责人:
Margarita Vigodner
金额:
$10.35万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2013
资助国家:
美国
项目状态:
已结题
起止时间:
2013-12-06 至 2015-11-30
关键词:
Administrative SupplementAdultAffectAmino AcidsAneuploidyAnimalsAntibodiesAreaBioinformaticsBiological ProcessBuffersCell Culture TechniquesCell LineCell SeparationCell physiologyCellsCellular biologyCo-ImmunoprecipitationsComplexCongenital AbnormalityContraceptive AgentsCouplesCytolysisDataDevelopmentDiseaseEnvironmentEventFailureFoundationsFundingGermGerm CellsGoalsHealthcareHourHumanHuman ResourcesHurricaneImmunoprecipitationIndividualInfertilityKnowledgeLaboratoriesLeadMale InfertilityMalignant neoplasm of testisMass Spectrum AnalysisMediatingMeiosisMitoticModificationMusNatureNew EnglandNew York CityPathway interactionsPopulationProceduresProteinsProteomeProteomicsProtocols documentationPublishingReagentRegulationReproductionResearchResearch PersonnelRoleSamplingSmall Ubiquitin-Related Modifier ProteinsSomatic CellSourceSpermatidsSpermatocytesSpermatogenesisSpermatogenic CellSpermatogoniaSpontaneous abortionStable Isotope LabelingStagingTesticular TissueTestingTestisTimeUbiquitinUniversitiesWestern BlottingWorkbasecell typecrosslinkexperienceimprovedinhibitor/antagonistinnovationisopeptidaseliquid chromatography mass spectrometrymalemeltingnovelnovel therapeuticsrepairedresearch studyundergraduate student
中文摘要
点击翻译按钮获取中文摘要
英文摘要
The research progress of our lab was seriously affected by the hurricane Sandy that devastated
the northeastern United States, especially New York City. On October 29th 2012, lower
Manhattan, more specifically the area to the south of 40th Street suffered from an unexpected
major power outage that lasted for a week. During this period, the power to the laboratory was
completely shut down. All the fridges and freezers in the laboratory remained off during the
power outage due to the lack of a backup generator in the building. A -80°C freezer’s
compressor was damaged due to the sudden power outage and the freezer had to be sent out
for repair afterwards. The major power outage caused by hurricane Sandy caused severe
losses to our laboratory materials. Lysates and cells, including costly cell line stocks, were
stored in the -80°C freezer. These valuable materials thawed and melted and were therefore
damaged. Some of the lysate samples are very difficult to produce and collect. These samples
required large amounts of time, work and expense to manufacture. They were generated from
a large number of experimental animals including adult and pubertal mice through several
rounds of a 10 hour STA-PUT separation procedure and were stored in the freezer for following
immunoprecipitations (IP) and mass-spectrometry identifications. Numerous antibodies were
required to confirm those IP experiments, all of which were stored in either a -20°C freezer or
4°C fridge. Consequently these antibodies were also spoiled during the power outage.
Additionally, all reagents useful for the SUMO project (such as lysis buffer, media, western blot
reagents etc.) were stored in the fridges and freezers and were also ruined.
Due to the aforementioned losses caused by “Sandy”, more funds will be needed to replace the
reagents, antibodies and personnel time.
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DOI:
10.1530/rep-15-0239
发表时间:
2016-02
期刊:
Reproduction (Cambridge, England)
影响因子:
--
作者:
[Xiao Y, Pollack D, Andrusier M, Levy A, Callaway M, Nieves E, Reddi P, Vigodner M]
通讯作者:
Vigodner M
DOI:
10.1016/j.bbrc.2017.04.107
发表时间:
2017-06-03
期刊:
Biochemical and biophysical research communications
影响因子:
3.1
作者:
[Xiao Y, Lucas B, Molcho E, Vigodner M]
通讯作者:
Vigodner M
Inhibition of CDK1 activity by sumoylation.
通过sumoylation抑制CDK1活性。
DOI:
10.1016/j.bbrc.2016.08.051
发表时间:
2016-09-16
期刊:
Biochemical and biophysical research communications
影响因子:
3.1
作者:
[Xiao Y, Lucas B, Molcho E, Schiff T, Vigodner M]
通讯作者:
Vigodner M
DOI:
10.4103/aja.aja_11_20
发表时间:
2020-11
期刊:
Asian journal of andrology
影响因子:
2.9
作者:
[Vigodner M, Lucas B, Kemeny S, Schwartz T, Levy R]
通讯作者:
Levy R
DOI:
10.1016/j.ab.2014.11.006
发表时间:
2015-05-15
期刊:
ANALYTICAL BIOCHEMISTRY
影响因子:
2.9
作者:
[Xiao, Yuxuan, Pollack, Daniel, Nieves, Edward, Winchell, Aby, Callaway, Myrasol, Vigodner, Margarita]
通讯作者:
Vigodner, Margarita
Sumoylation and its regulation in testicular Sertoli cells
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批准号:10654204
-
项目类别:
-
资助金额:$45.0万
-
财政年份:2023
-
负责人:Margarita Vigodner
-
依托单位:
Recover Losses Due to COVID-19 Pandemic
-
批准号:10530750
-
项目类别:
-
资助金额:$4.5万
-
财政年份:2022
-
负责人:Margarita Vigodner
-
依托单位:
Regulation of spermatogenesis by sumoylation
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批准号:8180188
-
项目类别:
-
资助金额:$49.67万
-
财政年份:2011
-
负责人:Margarita Vigodner
-
依托单位:
海外基金