An Ex Vivo Model of HIV-1 Latency and Reactivation using Primary Memory Cells

使用原代记忆细胞的 HIV-1 潜伏期和再激活的离体模型

• 批准号: 8613428
• 负责人: VICENTE PLANELLES
• 金额: $ 36.88万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-15 至 2015-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8613428
• 关键词: Agonist; Antigens; Area; Biological Process; Biology; CD4 Positive T Lymphocytes; Cells; Effector Cell; Elements; Event; Experimental Models; Future; Gene Expression; Genes; Goals; HIV; HIV-1; Human; Individual; Infection; Inflammation; Inflammatory; Knowledge; Latent Virus; Life; Memory; Modeling; Molecular; Physiological; Process; Proviruses; Regulation; Relative (related person); Signal Pathway; Signal Transduction; Stimulus; Study models; System; T memory cell; T-Lymphocyte Subsets; Trans-Activators; Vesicular stomatitis Indiana virus; Viral; Viral Genes; Virus; Virus Latency; cell type; cytokine; in vivo; latent infection; novel; permissiveness; public health relevance; reactivation from latency; transcription factor; translational study

DESCRIPTION (provided by applicant): The proposed studies use a novel experimental model of viral latency and reactivation that utilizes primary memory cells as targets. Using this system, we plan to understand in detail the mechanisms that HIV-1 uses to establish latency in vivo, as well as the physiological signals that trigger viral reactivation. Knowledge from these areas will be applicable in future translational studies that will seek compounds mimicking or antagonizing the relevant signaling pathways, with the ultimate goal of destroying the latent reservoir.

描述（由申请人提供）：拟议的研究使用了一种新的病毒潜伏和重新激活实验模型，该模型利用初级记忆细胞作为目标。利用这个系统，我们计划详细了解 HIV-1 在体内建立潜伏期的机制，以及触发病毒重新激活的生理信号。这些领域的知识将适用于未来的转化研究，这些研究将寻找模拟或拮抗相关信号通路的化合物，最终目标是破坏潜在的储存库。

项目成果

Studies of HIV-1 latency in an ex vivo model that uses primary central memory T cells.

• DOI: 10.1016/j.ymeth.2010.10.002
• 发表时间: 2011-01
• 期刊: METHODS
• 影响因子: 4.8
• 作者: Bosque, Alberto;Planelles, Vicente
• 通讯作者: Planelles, Vicente

An Ounce of Tat Prevention Is Worth a Pound of Functional Cure.

一盎司的预防胜过一磅的功能性治疗。

• DOI: 10.1128/mbio.01269-15
• 发表时间: 2015
• 期刊: mBio
• 影响因子: 6.4
• 作者: Planelles,Vicente

Reactivation of latent HIV-1 in central memory CD4⁺ T cells through TLR-1/2 stimulation.

• DOI: 10.1186/1742-4690-10-119
• 发表时间: 2013-10-24
• 期刊: Retrovirology
• 影响因子: 3.3
• 作者: Novis CL;Archin NM;Buzon MJ;Verdin E;Round JL;Lichterfeld M;Margolis DM;Planelles V;Bosque A
• 通讯作者: Bosque A