Buoyancy based bioseparations

基于浮力的生物分离

• 批准号: 8908230
• 负责人: Michael Benchimol
• 金额: $ 2.5万
• 依托单位: DIAGNOLOGIX, LLC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8908230
• 关键词: Adoption; Arts; Award; Biocompatible; Biological; Biological Assay; Biomedical Research; Blood; Blood Volume; California; Cancer Diagnostics; Cell Separation; Cell Survival; Cells; Clinical; Clinical Research; Complex; Culture Media; Data; Development; Devices; Diagnosis; Diagnostic; Disease; Funding; Gases; General Population; Harvest; Healthcare Systems; Human Resources; Laboratories; Leukocytes; Liquid substance; Magnetism; Mails; Marketing; Methods; Microbubbles; Modeling; Molecular; Molecular Analysis; Monitor; Neoplasm Circulating Cells; Penetration; Phase; Plastics; Process; Property; Protein Analysis; Publishing; RNA analysis; Recovery; Sampling; Small Business Innovation Research Grant; Solid; Speed; System; Techniques; Technology; Testing; Therapeutic; Time; Ultrasonography; United States National Institutes of Health; Universities; base; cell growth; cell type; cost; density; flexibility; genetic analysis; improved; innovation; interest; magnetic beads; meetings; neoplastic cell; novel; operation; primary care setting; prototype; public health relevance; tissue culture; user-friendly

Abstract There is an unmet need for cost-efficient technologies for high purity isolation of rare circulating tumor cells (CTCs) from blood. An ideal isolation technique should be able to meet the following criteria: (a) minimal processing time; (b) low final sample volume; (c) isolation from large blood volume; (d) low carryover of leukocytes and blood components; (e) high cell viability; (f) compatibility with downstream processes such as PCR or tissue culturing; (g) low cost and ease of operation by entry-level personnel. While there are numerous approaches for CTC isolation, none of them combines simplicity, low cost, efficiency and speed. Previously, we developed a novel process of buoyant microbubble-assisted isolation of rare tumor cells from blood and buffy coats. MBs efficiently (>80% recovery) and fast (less than 20 min) isolated rare tumor cells from 7 ml blood or 22 ml buffy coats. For Phase I, Diagnologix, LLC has partnered with the University of California San Diego in order to develop and test a prototype of an intuitive and user-friendly separation/harvesting device that in combination with MBs will be used for harvesting rare tumor cells from complex biological fluids (e.g., blood). The proposed setup will enable easy washing, immunostaining, genetic analysis or culturing of isolated CTCs. The most important competitive advantages of MB approach vs. magnetic beads would be high speed, sample purity, simplicity, flexibility, minimal final sample volume and low cost. The value proposition of the technology is in the qualitative and quantitative improvement in CTC isolation and analysis for biomedical and clinical research and potentially for clinical diagnostics.

抽象的 对于高纯度隔离的稀有技术，需要提高成本效益的技术 血液中循环肿瘤细胞（CTC）。理想的隔离技术应该能够 满足以下标准：（a）最小的处理时间； （b）最终样品量低； （C） 与大血容量隔离； （d）白细胞和血液的结转率低 成分; （e）高细胞活力； （f）与下游过程的兼容性 PCR或组织培养； （g）入门级人员的低成本和易于操作。 虽然有许多CTC隔离方法，但它们都没有结合 简单，低成本，效率和速度。以前，我们开发了一个新颖的过程 从血液和Buffy大衣中鲁s辅助分离的微生物辅助分离。 MB有效（恢复> 80％）和快速（少于20分钟）孤立的稀有肿瘤细胞 从7毫升血液或22毫升Buffy毛衣。对于第一阶段，Diagnologix，LLC与 加州大学圣地亚哥分校，以开发和测试 与MBS结合使用的直观和用户友好的分离/收获设备 用于从复杂的生物液（例如血液）中收集稀有肿瘤细胞。这 拟议的设置将使易于洗涤，免疫染色，遗传分析或培养 孤立的CTC。 MB方法与最重要的竞争优势。 磁珠将是高速，样品纯度，简单，灵活性，最小的最终 样本量和低成本。该技术的价值主张是定性的 以及生物医学和临床的CTC分离和分析的定量改进 研究并有可能进行临床诊断。