Buoyancy based bioseparations

基于浮力的生物分离

• 批准号: 8908230
• 负责人: Michael Benchimol
• 金额: $ 2.5万
• 依托单位: DIAGNOLOGIX, LLC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8908230
• 关键词: Adoption; Arts; Award; Biocompatible; Biological; Biological Assay; Biomedical Research; Blood; Blood Volume; California; Cancer Diagnostics; Cell Separation; Cell Survival; Cells; Clinical; Clinical Research; Complex; Culture Media; Data; Development; Devices; Diagnosis; Diagnostic; Disease; Funding; Gases; General Population; Harvest; Healthcare Systems; Human Resources; Laboratories; Leukocytes; Liquid substance; Magnetism; Mails; Marketing; Methods; Microbubbles; Modeling; Molecular; Molecular Analysis; Monitor; Neoplasm Circulating Cells; Penetration; Phase; Plastics; Process; Property; Protein Analysis; Publishing; RNA analysis; Recovery; Sampling; Small Business Innovation Research Grant; Solid; Speed; System; Techniques; Technology; Testing; Therapeutic; Time; Ultrasonography; United States National Institutes of Health; Universities; base; cell growth; cell type; cost; density; flexibility; genetic analysis; improved; innovation; interest; magnetic beads; meetings; neoplastic cell; novel; operation; primary care setting; prototype; public health relevance; tissue culture; user-friendly

Abstract There is an unmet need for cost-efficient technologies for high purity isolation of rare circulating tumor cells (CTCs) from blood. An ideal isolation technique should be able to meet the following criteria: (a) minimal processing time; (b) low final sample volume; (c) isolation from large blood volume; (d) low carryover of leukocytes and blood components; (e) high cell viability; (f) compatibility with downstream processes such as PCR or tissue culturing; (g) low cost and ease of operation by entry-level personnel. While there are numerous approaches for CTC isolation, none of them combines simplicity, low cost, efficiency and speed. Previously, we developed a novel process of buoyant microbubble-assisted isolation of rare tumor cells from blood and buffy coats. MBs efficiently (>80% recovery) and fast (less than 20 min) isolated rare tumor cells from 7 ml blood or 22 ml buffy coats. For Phase I, Diagnologix, LLC has partnered with the University of California San Diego in order to develop and test a prototype of an intuitive and user-friendly separation/harvesting device that in combination with MBs will be used for harvesting rare tumor cells from complex biological fluids (e.g., blood). The proposed setup will enable easy washing, immunostaining, genetic analysis or culturing of isolated CTCs. The most important competitive advantages of MB approach vs. magnetic beads would be high speed, sample purity, simplicity, flexibility, minimal final sample volume and low cost. The value proposition of the technology is in the qualitative and quantitative improvement in CTC isolation and analysis for biomedical and clinical research and potentially for clinical diagnostics.

抽象的 对稀有物质高纯度分离的经济高效技术的需求尚未得到满足 血液中的循环肿瘤细胞（CTC）。理想的隔离技术应该能够 满足以下标准： (a) 最短处理时间； (b) 最终样品体积低； (三) 与大血量隔离； (d) 白细胞和血液残留量低 成分; (e) 高细胞活力； (f) 与下游工艺的兼容性，例如 PCR或组织培养； (g) 成本低且易于入门级人员操作。 尽管有多种 CTC 分离方法，但没有一种方法能将 CTC 结合起来。 简单、成本低、效率高、速度快。此前，我们开发了一种新颖的工艺 浮力微泡辅助从血液和血沉棕黄层中分离罕见肿瘤细胞。 MB 有效（>80% 回收率）且快速（少于 20 分钟）分离稀有肿瘤细胞 来自 7 毫升血液或 22 毫升血沉棕黄层。对于第一阶段，Diagnologix, LLC 与 加州大学圣地亚哥分校为了开发和测试一个原型 直观且用户友好的分离/收获设备，与 MB 结合使用 可用于从复杂的生物液体（例如血液）中收获稀有肿瘤细胞。这 拟议的设置将能够轻松清洗、免疫染色、遗传分析或培养 孤立的 CTC。 MB 方法与传统方法相比最重要的竞争优势 磁珠速度快、样品纯度高、简单、灵活、最终结果最小 样品量大，成本低。该技术的价值主张在于定性 生物医学和临床 CTC 分离和分析的定量改进 研究和潜在的临床诊断。