Ex vivo analysis of irradiated finger and toe nails as a biodosimeter system Fla

达特茅斯大学U19 CMCR项目的目标是开发野外生物剂量学技术 这是基于体外和体内电子顺磁共振(EPR)光谱测量 在手指/脚趾指甲(“指甲”)或牙齿中的辐射诱导信号(RIS)。在本项目(2)中，体外EPR 测量钉子的方法朝着现场可展开的应用进一步发展。为了实现这一目标， 已经定义了四个目标，分别涉及信号表征、样品处理和信号 去除干扰信号的去卷积、测试方法的验证、识别和表征 有可能影响RIS信号的可变性的因素，并建立数据方法 适合外地非专家操作员和ER管理人员使用的采集、处理和输出 条件。表征整个信号、频谱分量并追求系统的 研究未辐照和辐照指甲中EPR信号的分子基础是很重要的 开发从机械感应信号中分离辐射感应信号的方法 Signal(管理信息系统)。这些研究的某些方面将利用项目3的数据，该项目将提供EPR 指甲的光谱，因此有助于更清楚地区分指甲的来源和性质 来自剪裁过程的信号。这一系列研究的结果是发展了一套 RIS和MIS信号的光谱分量，以及单独分量的衰变动力学 被整合到用于提取RIS分量信号的频谱去卷积过程中的信号。 RIS和MIS信号的可变性应根据指甲水分含量、人口统计数据进行表征 (例如，年龄、种族)、全身变量(怀孕、饮食/补充剂、指甲紊乱)和真菌感染。 最后，描述一种现场可用的样品采购方法，将样品放入 分光计，用于确定剂量的分析软件，以及如果存在需要描述的因素 在结果的校准中被考虑。虽然建造一个可现场部署的原型 满足中心的总体目标将与仪器核心合作开展，地址为 达特茅斯，这个项目将制定在开发和实施中实施的方法 建筑。

The objectives of the Dartmouth University U19 CMCR program are to develop field biodosimetry techniques that are based on ex vivo and in vivo electron paramagnetic resonance (EPR) spectrometry measurements of a radiation-induced signal (RIS) in finger/toe nails ("nails") or teeth. In this project (2), the ex vivo EPR method for measuring nails is further developed towards a field deployable application. To achieve this goal, four objectives have been defined that address signal characterization, sample treatment and signal deconvolution to remove interfering signals, validation of test methods, identification and characterization of factors that have the potential to impact the variability of the RIS signal, and establish methods for data acquisition, processing and output suitable for use by non-expert operators and ER managers under field conditions. Characterization of the overall signal, the spectral components and pursuing a systematic investigation of the molecular basis of the EPR signals in both unirradiated and irradiated nails is important towards developing approaches for separating the radiation-induced signal from a mechanically-induced signal (MIS). Some aspects of these studies will utilize the data from Project 3, which will provide EPR spectra of nails without clipping, and therefore help to differentiate more clearly the origin and nature of the signals from the clipping process. The result of this set of studies is the development of a basis set of spectral components of the RIS an MIS signals, coupled with the decay kinetics of the separate component signals that are integrated into a spectrum deconvolution procedure for extracting the RIS component signal. Variability in RIS and MIS signals are to be characterized with respect to nail water content, demographics (i.e. age, race), systemic variables (pregnancy, diet/supplements, nail disorders) and fungal infection. Finally, delineating a field-usable method for sample procurement, placement of the samples into the spectrometer, the analytic software to determine the dose, and delineation of factors that if present need to be considered in the calibration of the results. While the construction of a field-deployable prototype that meets the overall goals of the Center will be carried out in collaboration with the instrumental core at Dartmouth, this project will develop the methodology to be implemented in the development and construction.