Hollow Fiber Concentrating Pipette Tip for Improved Pathogen Detection in Foods

用于改进食品中病原体检测的中空纤维浓缩移液器吸头

• 批准号: 8592864
• 负责人: David S Alburty
• 金额: $ 29.78万
• 依托单位: INNOVAPREP, LLC
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8592864
• 关键词: Hollow; Fiber; Concentrating; Pipette; Tip

Abstract Rapid microbiological methods offer great potential for increasing the reliability of pathogen detection in foods while reducing labor costs and product hold times, however, small analysis volumes limit the usefulness of these methods. Pre-enrichment is often used to overcome this limitation, but the significant time advantage of these methods is then lost. Mechanical concentration or enrichment approaches hold promise, but their development has lagged. In an effort to fill this void, InnovaPrep has developed a rapid concentration approach with single-use membrane filter tips and a novel elution process to allow for straightforward, user- friendly concentration of pathogens from foods while eliminating cross contamination between samples. Through internal and Phase I project support InnovaPrep was able to release a commercial Concentrating Pipette (CP) device and flat membrane filter Concentrating Pipette Tips (CPTs) in March 2012 and the response has been strong - with 35 devices sold, two leased, and five loaned for evaluation. During Phase I and during the early months after release of the commercial CP system, it became clear that the approach was viable and that the market was ready to embrace this approach. It was, however, also apparent that the useful range of the current device was somewhat limited due to a relatively small membrane filter surface area of only 8.5 cm2. In particular, the upper sample volume size can be significantly limited when working with difficult food matrices. In Phase II, InnovaPrep proposes development of hollow fiber membrane filter CPTs (hf-CPTs) with up to 15 times more surface area than current flat membrane filter CPTs. This increased surface area will dramatically expand the useful range of the device, allowing for larger process volumes, greater concentration factors, reduced development time for new food methods, as well as allowing for the introduction of viral CPTs. Producing hf-CPTs at costs that fit the requirements of the food safety laboratory will require innovative manufacturing processes that can be automated. InnovaPrep has secured the help of Dr. Pierre C¿t¿ and Mr. Steven Pederson, two experts in the field of hollow fiber module design and production, to assist in the development of a novel static potting method in order to achieve these goals. A novel static potting method and new manufacturing processes will be developed and 3,500 beta hf-CPTs will be produced for use during the final Aims of the project. Using beta hf-CPTs, InnovaPrep will develop eleven concentration methods covering 11 high priority foods, five organisms, and six rapid detection methods. The developed methods will demonstrate the value of the CP over a range of food types, organisms and rapid detection methods and lay a broad based groundwork that serves as a starting point for development of additional methods by other users. The developed methods will be evaluated by at the University of Missouri - Columbia.

抽象的 快速微生物学方法为提高食品中病原体检测的可靠性提供了巨大潜力 然而，虽然减少了劳动力成本和产品保存时间，但分析量小限制了分析的有用性 这些方法。预富集通常用于克服这一限制，但其显着的时间优势 这些方法随后就会丢失。机械浓缩或浓缩方法很有希望，但它们的 发展已经滞后。为了填补这一空白，InnovaPrep 开发了一种快速浓缩剂 采用一次性膜过滤器吸头和新颖的洗脱工艺的方法，可实现简单、用户友好的操作 友好地浓缩食品中的病原体，同时消除样品之间的交叉污染。 通过内部和第一阶段项目支持，InnovaPrep 能够发布商业浓缩 2012 年 3 月推出的移液器 (CP) 装置和平板膜过滤器浓缩移液器吸头 (CPT) 以及 反响强烈 - 已售出 35 台设备，租赁两台设备，借出 5 台进行评估。第一阶段期间 在商业 CP 系统发布后的最初几个月里，很明显该方法是可行的 可行，并且市场已准备好接受这种方法。然而，同样明显的是，有用的 由于膜过滤器表面积相对较小，当前设备的范围受到一定限制 8.5 平方厘米。特别是，当处理困难时，上样本体积大小可能会受到显着限制。 食物矩阵。在第二阶段，InnovaPrep建议开发中空纤维膜过滤器CPT（hf-CPT） 表面积比当前的平板膜过滤器 CPT 大 15 倍。这种增加的表面积将 极大地扩展了设备的有用范围，允许更大的处理量、更高的浓度 因素，缩短了新食品方法的开发时间，以及允许引入病毒 CPT。 以符合食品安全实验室要求的成本生产 hf-CPT 需要创新 可以自动化的制造过程。 InnovaPrep 得到了 Pierre C¿t¿ 博士和 Mr. 的帮助。 Steven Pederson，两位中空纤维模块设计和生产领域的专家，协助 开发一种新颖的静态灌封方法以实现这些目标。一种新颖的静态灌封方法 将开发新的制造工艺，并生产 3,500 个 β hf-CPT 以供在 该项目的最终目标。 InnovaPrep 将使用 beta hf-CPT 开发 11 种浓缩方法，涵盖 11 种高优先级食品， 五种生物体和六种快速检测方法。所开发的方法将证明 CP 的价值 涵盖一系列食品类型、生物体和快速检测方法，并奠定了广泛的基础 作为其他用户开发其他方法的起点。所开发的方法将 由密苏里大学哥伦比亚分校进行评估。