Continuation of FERN Activities at the Connecticut Agricultural Experiment Statio

FERN 活动在康涅狄格州农业实验站继续进行

• 批准号: 8500128
• 负责人: Laurie J. Peterson-Wright
• 金额: $ 36万
• 依托单位: CONNECTICUT AGRICULTURAL EXPERIMENT STA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8500128
• 关键词: Continuation; FERN; Activities; Connecticut; Agricultural

CONTINUATION OF FERN ACTIVITIES AT THE CONNECTICUT AGRICULTURAL EXPERIMENT STATION (CAES) Contamination of the food supply from chemical terrorism or adulteration is an ongoing threat. The Food Emergency Response Laboratory Network (FERN) was established in direct response to this threat with the critical focus areas of prevention, preparedness, response and recovery. Specifically, there needs to be trained personnel with the expertise, supplies and instrumentation necessary to handle the redundancy and laboratory surge capacity that will be required to respond to incidences of food supply contamination. This proposal is in direct response to that need and will support a State laboratory's full participation within the FERN Chemistry Cooperative Agreement Program (cCAP). This participation includes instrumentation, training, method enhancement activities and analysis of surveillance samples. The Department of Analytical Chemistry at The Connecticut Agricultural Experiment Station (CAES) has a 115 year history of food safety research and over the last five years as a cCAP laboratory, has demonstrated the expertise and analytical capacity required to meet FERN goals. The current proposal is divided into four distinct project areas, all of which are inherently flexible depending on Forensic Chemistry Center (FCC) and FERN National Program Office (NPO) input: (1) The use of Gas Chromatography- Mass Spectrometry (GC-MS) for the analysis of unknown compounds in foods. We have used GC-MS to detect melamine in food products from China and pesticide residues in fruits and vegetables. We have enhanced the FDA FERN T021 methodology by developing the use of a Programmed Temperature Vaporizing (PTV) inlet for the GC-MS. In this proposal we look to continue our advances with this methodology by looking at new toxins and poisons, by expanding to new matrices such as alcohol and seafood, and to explore the potential use of Solid Phase Microextraction (SPME) with GC-MS. (2) The use of Liquid Chromatography - Mass Spectrometry (LC-MS) for the analysis of unknown compounds in foods. We have used LC-MS to analyze toxins in surveillance samples and pesticide residues in produce, as well as honey bee related matrixes. We have added new toxins to the FDA FERN T022 method and evaluated many matrixes. We propose the use of new chromatography columns to expand the capabilities of the FERN methodology and also to expand to additional matrices such as alcohol and seafood. (3) The use of Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) for the identification of toxic metals in foods. Our elemental experience has ranged from the determination of lead in maple syrup to cadmium in fish tissue to nanoparticles in vegetables. We have used the FERN methods for the analysis of food surveillance samples. We propose to further enhance methodology by optimizing the High Matrix Introduction accessory for the ICP - MS. We also propose to use LC prior to elemental analysis instrumentation so as to speciate metals and separately analyze multiple oxidative states. (4) The use of Enzyme -Linked Immunosorbent Assay (ELISA) for the identification of toxins in foods. We have used ELISA methods for surveillance samples, proficiency tests, and to determine peanut allergens in foods. We evaluated Tetracore Ricin kits and are developing techniques for Abrin analysis. We propose to use ELISA kits and lateral flow devices (LFDs) for matrixes specified by FDA as well as studies on the rosary pea, the source of Abrin. The explicit goal of this opportunity is to enable the analysis of food in the event that redundancy and surge capacity is need by FERN. The CAES Department of Analytical Chemistry has demonstrated the expertise, capability, and adaptability to fully meet and exceed these program needs.

康涅狄格州农业实验中蕨类活动的延续 车站(CAES) 化学恐怖主义或掺假对食品供应的污染是一个持续的威胁。 粮食紧急反应实验室网络(FERN)在直接反应中建立 应对这一威胁的关键重点领域是预防、准备、应对和恢复。 具体地说，需要训练有素的人员，具备专门知识、用品和仪器。 需要处理冗余和实验室浪涌容量，这将需要 应对食品供应污染事件。这项提议就是对此的直接回应 需要并将支持国家实验室全面参与蕨类化学 合作协议计划(CCAP)。这种参与包括仪器、培训、 方法开展强化活动，对监测样品进行分析。美国商务部 康涅狄格州农业实验站(CAES)的分析化学有115 一年的食品安全研究历史和过去五年作为CCAP实验室的经验， 展示了满足FERN目标所需的专业知识和分析能力。海流 提案分为四个不同的项目领域，所有这些领域都具有内在的灵活性，具体取决于 关于法医化学中心(FCC)和蕨类国家项目办公室(NPO)的投入： (1)用气相色谱-质谱仪(GC-MS)分析 食品中的未知化合物。我们已经用GC-MS检测了食品中的三聚氰胺 来自中国和水果蔬菜中的农药残留。我们已经增强了FDA的蕨类 T021通过开发使用程序升温汽化(PTV)入口的方法 用于气相色谱-质谱仪。在这份提案中，我们希望通过以下方式继续推进这一方法 研究新的毒素和毒物，通过扩展到酒精和海鲜等新的基质， 探索固相微萃取(SPME)与GC-MS联用的潜在应用。 (2)液-质联用(LC-MS)分析 食品中的未知化合物。我们已经使用LC-MS来分析监测中的毒素 样品和农产品中的农药残留，以及与蜜蜂有关的基质。我们有 在FDA蕨类T022方法中添加了新的毒素，并对许多基质进行了评估。我们建议 使用新的色谱柱来扩展蕨类方法学的能力 并扩大到酒精和海鲜等其他基质。 (3)电感耦合等离子体质谱(电感耦合等离子体质谱)测定 食品中有毒金属的鉴定。我们的基本经验从 测定枫糖浆中的铅、鱼组织中的镉、蔬菜中的纳米颗粒。 我们已使用蕨类方法对食品监测样本进行分析。我们建议 通过优化用于电感耦合等离子体的高矩阵引入附件，进一步提高方法学水平 女士我们还建议在元素分析仪器之前使用LC，以便指定 并分别分析多种氧化状态。 (4)用酶联免疫吸附试验(ELISA法)鉴定 食物中的毒素。我们已经使用了ELISA法来监测样本，熟练程度测试，以及 检测食品中的花生过敏原。我们评估了Tetracore Ricin试剂盒，并正在开发 Abrin分析技术。我们建议使用ELISA试剂盒和侧向流动装置(LFD) 用于FDA指定的基质，以及对阿布林的来源--念珠豌豆的研究。 这一机会的明确目标是在发生以下情况时能够分析食物 FERN需要冗余和浪涌容量。中国工程院分析系 化学已经证明了专业知识、能力和适应能力完全满足并超越 这些计划需要。