Flow Cytometry and High Throughput Screening Shared Resource

UNM共享流式细胞术和化学生物学资源由位于以下地点的实验室组成 北卡罗来纳大学癌症研究设施和研究孵化器大楼(RIB)，步行5分钟即可到达 细胞学和筛选组件由Bruce Edwards博士指导，Brandy操作 Crowder和Catherine Prudom分别是博士。细胞计数和筛查实验室流水线 可供癌症中心研究人员使用的细胞仪和其他设备。这个 资源开发了使用流式细胞仪进行高通量筛查的能力，这一能力已经得到发展 进入一个名为UNM分子发现中心的成熟的高通量筛选操作 这是美国国立卫生研究院路线图资助的分子图书馆探测生产中心网络的一部分。联席董事 Tudor Oprea在小分子计算和信息学方法方面领导合作努力 发现装在肋骨里。在本报告所述期间。癌症中心成员发表了70篇文章。 与资源使用的关联，包括仅基于计算方法的资源使用。二十六岁 代表所有4个癌症中心研究项目的成员使用了资源服务(占总使用量的80%) 与25项研究资助相结合。目前对资源用户的按存储容量使用计费处于 全国范围内的费率和癌症中心会员有资格在所有费率上享受20%的共同支付。资源用户是 接受过仪器和软件使用方面的个人培训。允许新用户通过以下方式执行试点项目 免收检测开发阶段的使用费。该资源提供细胞分析、细胞 将数据分类和离线数据分析作为标准服务。它最近推出了高通量筛选和 化学信息学，允许资源用户常规访问发明和开发的新型超细胞技术 在UNM由布鲁斯·爱德华兹和拉里·斯克拉尔开发，他们是癌症中心的副主任，基础 研究。该设施的总体目标是：a)向研究人员提供支助服务；b)培训 例如与个别研究人员的特别工作和专门领域的预定讲习班；c) 与癌症中心研究人员和受训人员合作，特别是在开发新研究方面 在小分子鉴定中的应用，d)通过发展高通量进行创新 384和1536孔板的使用能力和应用，化合物文库的筛选， 实施基于珠粒的多路分析，为化学生物学提供化学信息学支持 项目；以及e)通过设施参观、描述性海报展示、年度开放参观活动进行传播 以及资源网站的定期更新。

The UNM Shared Flow Cytometry and Chemical Biology Resource consists of laboratories located in the UNM Cancer Research Facility and The Research Incubator Building (RIB), 5 minutes walking distance The Cytometry and Screening components are directed by Bruce Edwards, Ph.D., and operated by Brandy Crowder and Catherine Prudom, Ph.D. respectively. The Cytometry and Screening Lab houses flow cytometers and other equipment that are available for use by researchers in the Cancer Center. The Resource developed the capability for high throughput screening with flow cytometers which has evolved into a full-fledged High Throughput Screening operation called the UNM Center for Molecular Discovery which is part of the NIH Roadmap funded Molecular Libraries Probe Production Center Network. Co-Director Tudor Oprea leads collaborative efforts in computational and informatics approaches to small molecule discovery housed in RIB. During the reporting period. Cancer Center members published >70 articles in association with resource usage including those based on computational approaches alone. Twenty-six members representing all 4 Cancer Center research programs used resource services (80% of total use) in conjunction with 25 research grants. Current charge backs to resource users are in the low to mid-range of nation-wide rates and Cancer Center members qualify for 20% co-pay on all rates. Resource users are individually trained in instrument and software use. New users are enabled to perform pilot projects through a waiver of use charges during the assay development phase. The Resource provides cell analysis, cell sorting, and offline data analysis as standard services. It recently introduced high throughput screening and cheminformatics, allowing resource users routine access to novel HyperCyt technology invented and developed at UNM by Bruce Edwards and Larry Sklar, the Cancer Center Associate Director of Basic Research. The overall goals of the facility are to provide: a) support services to research faculty; b) training such as ad hoc work with individual researchers and scheduled workshops in specialized areas; c) collaboration with Cancer Center investigators and trainees, particularly in the development of new research applications in small molecule identification, d) innovation through the development of high throughput capabilities and applications for use of 384 and 1536 well plates, screening of compound libraries, implementation of multiplexed bead-based analysis, and cheminformatics support for chemical biology projects; as well as e) dissemination through facility tours, descriptive poster displays, an annual open house and periodic updating of the Resource web site.