Animal Model and Genotyping Core Support Facility

小鼠模型动物和基因分型核心为癌症生物学和遗传学实验室的研究人员提供了多个领域的支持，所有这些领域都与B细胞淋巴瘤、骨髓瘤、浆细胞肿瘤发生、皮肤癌发生、黑色素瘤、乳腺癌和前列腺癌的小鼠模型有关，所有这些都是由该小组的各种研究人员开发的。在动物核心中，我们保持遗传完整性，并监督维持许多不同品系小鼠所需的所有育种策略。核心工作人员进行所有遗传监测/基因分型。我们常规进行DNA提取，通过PCR和凝胶电泳对所有小鼠品系进行基因分型。我们积极参与使用动物模型的各种实验的规划，设计，执行和分析，并建立和执行各种药物的抗肿瘤活性的临床前测试。我们还开始了一项生产育种计划，每周为实验室提供新生小鼠幼仔，以收获角质形成细胞。我们已经获得了Lumina II成像仪，现在可以使用生物发光技术进行肿瘤/药物研究。我们撰写了许多原始动物研究提案和相关修订案，并确保其保持最新并符合NCI动物护理和使用委员会的要求。我们积极参与ACUC和AALAC所有检查的准备工作。动物中心负责协调向世界各地的研究人员运送小鼠品系和肿瘤品系，从而为癌症研究界的其他人提供有用的研究工具。基因组学部分为实验室内的研究人员提供帮助，并在适当时在癌症研究中心，关于各种各样的基因组应用，包括但不限于数据库挖掘，标记开发，单核苷酸多态性（SNP）鉴定和分析，测序和序列同源性搜索。目前支持的程序包括MacVector、DS Gene、Vector NTI、LaserGene、Sequencher、Partek和Influencity Pathway Analysis。核心中动物和基因组专业知识的结合有助于我们的研究人员进行癌症发生，癌症进展和癌症转移的机制研究。

The mouse model animal and genotyping core provides support to investigators in the Laboratory of Cancer Biology and Genetics in several areas, all relating to mouse models of B cell lymphoma, myeloma, plasma cell tumorigenesis, skin carcinogenesis, melanoma, breast cancer and prostate cancer, all of which have been developed by various investigators in the group. In the animal core, we maintain the genetic integrity and oversee all of the breeding strategies required to maintain the many different lines of mice. The core staff carries out all genetic monitoring/genotyping. We routinely perform DNA extraction, genotyping of all mouse lines by PCR and gel electrophoresis. We actively participate in the planning, design, execution and analysis of various types of experiments using animal models and in setting up and executing preclinical testing of various drugs for their anti-tumor activity. We have also begun a production breeding program to provide the lab with neonatal mouse pups on a weekly basis for the harvest of keratinocytes. We have acquired an Lumina II imaging machine and can now carry out tumor/drug studies using bioluminescence technology. We write many of the original Animal Study Proposals and associated amendments, and assure that they are kept up to date and in compliance with the NCI Animal Care and Use Committee. We actively participate in preparation for all inspections by the ACUC and AALAC. The animal core is responsible for coordinating the shipment of mouse lines as well as tumor lines to investigators all over the world, thus providing useful research tools to others in the cancer research community. The genomics section provides assistance to investigators within the laboratory, and in the Center for Cancer Research when appropriate, with respect to a wide variety of genomic applications including, but not limited to database mining, marker development, single nucleotide polymorphism (SNP) identification and analysis, sequencing and sequence homology searches. Programs currently supported include MacVector, DS Gene, Vector NTI, LaserGene, Sequencher, Partek, and Ingenuity Pathway Analysis. The combination of animal and genomic expertise in the core assists our investigators in their mechanistic studies of cancer initiation, cancer progression and cancer metastasis.