Animal Model and Genotyping Core Support Facility

动物模型和基因分型核心支持设施

• 批准号: 7970017
• 负责人: Wendy Du bois
• 金额: $ 128.06万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7970017
• 关键词: Amendment; Animal Care and Use Committees; Animal Genetics; Animal Model; Animals; Area; B-Cell Lymphomas; Breeding; Cancer Biology; Communities; Computer software; DNA; Databases; Development; Equipment; Foundations; Genes; Genetic; Genomics; Genotype; Goals; Laboratories; Malignant Neoplasms; Malignant neoplasm of prostate; Mining; Monitor; Multiple Myeloma; Mus; NCI Center for Cancer Research; National Cancer Institute; Neoplasm Metastasis; Pathway Analysis; Pharmaceutical Preparations; Plasma Cell Neoplasm; Preclinical Testing; Preparation; Research; Research Personnel; Research Support; Sequence Analysis; Sequence Homology; Single Nucleotide Polymorphism; Skin Carcinogenesis; Techniques; Writing; anticancer research; cancer initiation; design; gel electrophoresis; malignant breast neoplasm; melanoma; mouse model; new technology; programs; research study; tool; tumor; tumor progression; tumorigenesis; vector

The mouse model animal and genotyping core provides support to investigators in the Laboratory of Cancer Biology and Genetics in several areas, all relating to mouse models of B cell lymphoma, myeloma, plasma cell tumorigenesis, skin carcinogenesis, melanoma, breast cancer and prostate cancer, all of which have been developed by various investigators in the group. In the animal core, we maintain the genetic integrity and oversee all of the breeding strategies required to maintain the many different lines of mice. The core staff carries out all genetic monitoring/genotyping. We routinely perform DNA extraction, genotyping of all mouse lines by PCR and gel electrophoresis. We actively participate in the planning, design, execution and analysis of tumor induction experiments and in setting up and executing preclinical testing of various drugs for their anti-tumor activity. We write many of the original Animal Study Proposals and associated amendments, and assure that they are kept up to date and in compliance with the NCI Animal Care and Use Committee. We actively participate in preparation for all inspections by the ACUC and AALAC. The animal core is responsible for coordinating the shipment of mouse lines as well as tumor lines to investigators all over the world, thus providing useful research tools to others in the cancer research community. The genomics section provides assistance to investigators within the laboratory, and in the Center for Cancer Research when appropriate, with respect to a wide variety of genomic applications including, but not limited to database mining, marker development, single nucleotide polymorphism (SNP) identification and analysis, sequencing and sequence homology searches. Programs currently supported include MacVector, DS Gene, Vector NTI, LaserGene, Sequencher, Partek, and Ingenuity Pathway Analysis. The combination of animal and genomic expertise in the core assists our investigators in their mechanistic studies of cancer initiation, cancer progression and cancer metastasis.

小鼠模型动物和基因分型核心为研究人员提供支持， 癌症生物学和遗传学实验室的几个领域，都涉及到小鼠模型的B 细胞淋巴瘤、骨髓瘤、浆细胞肿瘤发生、皮肤癌发生、黑色素瘤、乳腺癌 癌症和前列腺癌，所有这些都是由不同的研究人员开发的， 组在动物的核心，我们保持基因的完整性，并监督所有的育种 维持不同品系小鼠所需的策略。核心员工执行 所有基因监测/基因分型。我们常规进行DNA提取，基因分型， 小鼠品系进行PCR和凝胶电泳。我们积极参与规划，设计， 肿瘤诱导实验的执行和分析以及设置和执行 各种药物的抗肿瘤活性的临床前测试。我们写了很多 原始动物研究提案和相关修正案，并确保其符合 日期，并符合NCI动物护理和使用委员会的要求。我们积极参与 为ACUC和AALAC的所有检查做准备。动物核心负责 协调小鼠系和肿瘤系的运输， 世界，从而为癌症研究界的其他人提供有用的研究工具。的 基因组学部分为实验室和中心的研究人员提供协助 在适当的情况下，在各种基因组应用方面， 包括但不限于数据库挖掘、标记开发、单核苷酸 多态性（SNP）鉴定和分析、测序和序列同源性搜索。 目前支持的程序包括MacVector，DS Gene，Vector NTI，LaserGene，Sequencher， Partek，和Insufficiency Pathway Analysis。动物和基因组专业知识的结合， 核心协助我们的研究人员在他们的机制研究癌症的启动，癌症 进展和癌症转移。