Animal Model and Genotyping Core Support Facility

动物模型和基因分型核心支持设施

• 批准号: 8158358
• 负责人: Wendy Du bois
• 金额: $ 130.62万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8158358
• 关键词: Animal Model; Genotype

The mouse model animal and genotyping core provides support to investigators in the Laboratory of Cancer Biology and Genetics in several areas, all relating to mouse models of B cell lymphoma, myeloma, plasma cell tumorigenesis, skin carcinogenesis, melanoma, breast cancer and prostate cancer, all of which have been developed by various investigators in the group. In the animal core, we maintain the genetic integrity and oversee all of the breeding strategies required to maintain the many different lines of mice. The core staff carries out all genetic monitoring/genotyping. We routinely perform DNA extraction, genotyping of all mouse lines by PCR and gel electrophoresis. We actively participate in the planning, design, execution and analysis of tumor induction experiments and in setting up and executing preclinical testing of various drugs for their anti-tumor activity. We have acquired an Lumina II imaging machine and can now carry out tumor/drug studies using bioluminescence technology. We write many of the original Animal Study Proposals and associated amendments, and assure that they are kept up to date and in compliance with the NCI Animal Care and Use Committee. We actively participate in preparation for all inspections by the ACUC and AALAC. The animal core is responsible for coordinating the shipment of mouse lines as well as tumor lines to investigators all over the world, thus providing useful research tools to others in the cancer research community. The genomics section provides assistance to investigators within the laboratory, and in the Center for Cancer Research when appropriate, with respect to a wide variety of genomic applications including, but not limited to database mining, marker development, single nucleotide polymorphism (SNP) identification and analysis, sequencing and sequence homology searches. Programs currently supported include MacVector, DS Gene, Vector NTI, LaserGene, Sequencher, Partek, and Ingenuity Pathway Analysis. The combination of animal and genomic expertise in the core assists our investigators in their mechanistic studies of cancer initiation, cancer progression and cancer metastasis.

小鼠模型动物和基因分型核心在几个领域为癌症生物学和遗传学实验室的研究人员提供支持，所有这些都与B细胞淋巴瘤、骨髓瘤、浆细胞肿瘤发生、皮肤癌、黑色素瘤、乳腺癌和前列腺癌的小鼠模型有关，所有这些都是由该小组的不同研究人员开发的。在动物核心中，我们保持遗传完整性，并监督维持许多不同品系的小鼠所需的所有育种策略。核心工作人员进行所有基因监测/基因分型。我们常规进行DNA提取，用聚合酶链式反应和凝胶电泳法对所有小鼠品系进行基因分型。我们积极参与肿瘤诱导实验的计划、设计、执行和分析，以及建立和执行各种抗肿瘤活性药物的临床前测试。我们已经获得了一台Lumina II成像机，现在可以使用生物发光技术进行肿瘤/药物研究。我们撰写了许多原始的动物研究建议和相关修订，并保证它们是最新的，并符合NCI动物保护和使用委员会的要求。我们积极参与ACUC和AALAC所有检查的准备工作。动物核心负责协调将小鼠品系以及肿瘤品系运送到世界各地的研究人员手中，从而为癌症研究界的其他人提供有用的研究工具。基因组学部分在广泛的基因组应用方面为实验室内和癌症研究中心的研究人员提供适当的协助，这些应用包括但不限于数据库挖掘、标记开发、单核苷酸多态(SNP)鉴定和分析、测序和序列同源性搜索。目前支持的程序包括MacVECTOR、DS gene、VECTOR NTI、Lasergene、Sequencher、Partek和Invenity Path Analyst。核心中的动物和基因组专业知识的结合有助于我们的研究人员对癌症起始、癌症进展和癌症转移的机制研究。