CELLULAR IMAGING, SURGERY, AND TISSUE PROCESSING CORE FACILITY

细胞成像、手术和组织处理核心设施

• 批准号: 8562593
• 负责人: Robert E Fyffe
• 金额: $ 21.54万
• 依托单位: WRIGHT STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8562593
• 关键词: Achievement; Administrative Supplement; Administrator; Cataloging; Catalogs; Collaborations; Control Animal; Core Facility; Data; Data Analyses; Data Set; Data Storage and Retrieval; Development; Electron Microscope; Electron Microscopy; Electrons; Electrophysiology (science); Ensure; Fluorescence; Fostering; Funding; Goals; Histology; Human Resources; Image; Image Analysis; Immunohistochemistry; Individual; Injection of therapeutic agent; Instruction; Intramuscular; Investments; Label; Laboratories; Light; Microinjections; Microscope; Microscopic; Microsurgery; Modeling; Muscle; Nerve; Neurons; Operative Surgical Procedures; Performance; Peripheral nerve injury; Postdoctoral Fellow; Procedures; Proteins; Qualifying; Recovery; Research; Research Personnel; Resources; Rodent; Secure; Services; Slide; Spinal Cord; Synapses; System; Technical Expertise; Techniques; Tissues; Tracer; Training; United States National Institutes of Health; Update; cellular imaging; cost effective; cryostat; data acquisition; data sharing; design; digital; experience; image processing; improved; injury and repair; instrument; instrumentation; meetings; member; nerve injury; repaired; research study; synaptic function; tissue processing

PROJECT SUMMARY (See Instructions): Core B is essential to support the microscopic imaging, quantitative analysis, and peripheral nerve injury model used in this PPG. The Core is used extensively by all three projects and contributes directly to multiple goals of these projects. The scope and resources of Core B have been significantly expanded by addition or update of new instrumentation (e.g. an AMT digital camera for the electron microscope, funded by an Administrative Supplement to the PPG) to meet the needs of the PPG projects. A significant addition is the provision of expertise and technical help to provide a single model of peripheral nerve injury in rodents that is used by all 3 projects. This eliminates variability between projects in surgical technique and microinjection of tracers in specific muscles or nerves, and facilitates direct comparison of data between projects to aid in interpretation. The Core facility is directed by a highly experienced investigator and research administrator, assisted by three highly trained and qualified research associates. The Core provides all necessary training to members of the PPG groups for authorized access and use of Core instruments and facilities; PPG members have priority access to the facility. Services include training and guidance for use of instruments and analytical techniques, assistance with design and/or interpretation of experiments, hands-on performance of tissue processing and imaging as required, electron microscopy processing and imaging, and microsurgery and tracer injection. Significant resources include 3 confocal microscopes (one is a multiphoton instrument with electrophysiology setup), electron microscope suite, Neurolucida system, fluorescence dissecting microscope, cryostats, vibratomes and ultramicrotomes, cryosubstitution system, a fully equipped histology laboratory, access to a state-of-the-art suite for microsurgery in rodents, and four off-line workstations for image processing and data analysis. Data is securely stored, and each PPG PI has secure access to a PPG shared data Cloud (1.0 terabyte) where they can access their own and each other's data and communicate seamlessly with the data acquisition instruments in the Core.

项目总结（参见说明）：核心B对于支持本PPG中使用的显微镜成像、定量分析和周围神经损伤模型至关重要。核心被所有三个项目广泛使用，并直接有助于这些项目的多个目标。核心项目B的范围和资源因增加或更新了新的仪器（例如，电子显微镜用先进制造技术数码相机，由项目编制补助金的行政补助金供资）而大大扩大，以满足项目编制补助金项目的需要。一个重要的补充是提供专业知识和技术帮助，以提供所有3个项目使用的啮齿动物外周神经损伤的单一模型。这消除了手术技术和特定肌肉或神经中微量注射示踪剂的项目之间的差异，并促进了项目之间数据的直接比较，以帮助解释。核心设施由一位经验丰富的研究员和研究管理员领导，并由三名训练有素的合格研究助理协助。核心为PPG团体成员提供所有必要的培训，以便授权访问和使用核心仪器和设施; PPG成员有权优先访问该设施。服务包括培训和指导使用仪器和分析技术，协助设计和/或解释实验，根据需要动手进行组织处理和成像，电子显微镜处理和成像，显微外科手术和示踪剂注射。重要的资源包括3个共聚焦显微镜（一个是多光子仪器与电生理设置），电子显微镜套件，Neurolucida系统，荧光解剖显微镜，低温恒温器，振动器和超微切片机，冷冻置换系统，一个设备齐全的组织学实验室，进入国家的最先进的套房显微外科啮齿动物，和四个离线工作站的图像处理和数据分析。数据被安全存储，每个PPG PI都可以安全访问PPG共享数据云（1.0 TB），他们可以访问自己和彼此的数据，并与核心中的数据采集仪器无缝通信。