JHU Center for Neuroscience Research

约翰霍普金斯大学神经科学研究中心

• 批准号: 8441558
• 负责人: PAUL F WORLEY
• 金额: $ 19.63万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8441558
• 关键词: Alleles; Award; Basic Science; Commit; DNA; Floor; Funding; Gene Targeting; Generations; Genes; Genetic Models; Germ Lines; Holly; Individual; Injection of therapeutic agent; Institutes; Institution; Journals; Knock-in Mouse; Knock-out; Knockout Mice; Laboratories; Letters; Manuscripts; Measures; Mus; National Institute of Neurological Disorders and Stroke; Neurons; Neurosciences; Neurosciences Research; Peer Review; Persons; Postdoctoral Fellow; Protocols documentation; Publications; Research; Research Personnel; Science; Services; Time; Transgenic Animals; Transgenic Organisms; United States National Institutes of Health; Wood material; Work; animal facility; blastocyst; cost; design; embryonic stem cell; experience; graduate student; homologous recombination; interest; novel; operation; success; transmission process

EMBRYONIC STEM CELL CORE CI B1. Establishment of the ES Cell Core: The ES Cell Core was founded with the support of this award and began operations -four years ago. It is located on the 9th Floor of the Wood Basic Science Building in -500 sq. ft. of space that is contiguous with the JHU SOM Transgenic Animal Facility. Dr. Woriey serves as the Director of the ES Cell Core and works closely with Dr. Roger Reeves, Director of the Transgenic Animal Facility, to integrate the functions of these Cores (see letter of support from Dr. Reeves (consultant)). Ms. Holly Wellington serves as Core Manager for this facility. The ES Cell Core reviews the design of targeting constructs, electroporates targeting construct DNA into ES cells, and then selects and amplifies ES cell colonies in duplicate 96 well plates; one plate is cryogenically stored and the other provided to investigators to screen for homologous recombination. The Core then grows promising ES cell clones, checks karyotypes and provides the targeted ES cells to the Transgenic Facility for injection into blastocysts.

胚胎干细胞核心 CIB1 ES细胞核心的建立： ES Cell Core在该奖项的支持下成立，并于四年前开始运营。它位于木材基础科学大楼的9楼，面积为-500平方米。英尺与JHU SOM转基因动物设施相邻的空间。Wariey博士担任ES细胞核心的主任，并与转基因动物设施主任Roger Reeves博士密切合作，以整合这些核心的功能。 (see Reeves博士（顾问）的支持信）。冬青惠灵顿女士担任该设施的核心经理。ES Cell Core审查靶向构建体的设计，将靶向构建体DNA电穿孔到ES细胞中，然后在一式两份的96孔板中选择和扩增ES细胞集落;一个板低温储存，另一个板提供给研究者以筛选同源重组。然后，核心培养有希望的ES细胞克隆，检查核型，并将靶向的ES细胞提供给 用于注射到胚泡中的转基因设施。