JHU Center for Neuroscience Research

约翰霍普金斯大学神经科学研究中心

• 批准号: 8214189
• 负责人: PAUL F WORLEY
• 金额: $ 19.19万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8214189
• 关键词: Alleles; Award; Basic Science; Commit; DNA; Floor; Funding; Gene Targeting; Generations; Genes; Genetic Models; Germ Lines; Holly; Individual; Injection of therapeutic agent; Institutes; Institution; Journals; Knock-in Mouse; Knock-out; Knockout Mice; Laboratories; Letters; Manuscripts; Measures; Mus; National Institute of Neurological Disorders and Stroke; Neurons; Neurosciences; Neurosciences Research; Peer Review; Persons; Postdoctoral Fellow; Protocols documentation; Publications; Research; Research Personnel; Science; Services; Time; Transgenic Animals; Transgenic Organisms; United States National Institutes of Health; Wood material; Work; animal facility; blastocyst; cost; design; embryonic stem cell; experience; graduate student; homologous recombination; interest; novel; operation; success; transmission process

EMBRYONIC STEM CELL CORE CI B1. Establishment of the ES Cell Core: The ES Cell Core was founded with the support of this award and began operations -four years ago. It is located on the 9th Floor of the Wood Basic Science Building in -500 sq. ft. of space that is contiguous with the JHU SOM Transgenic Animal Facility. Dr. Woriey serves as the Director of the ES Cell Core and works closely with Dr. Roger Reeves, Director of the Transgenic Animal Facility, to integrate the functions of these Cores (see letter of support from Dr. Reeves (consultant)). Ms. Holly Wellington serves as Core Manager for this facility. The ES Cell Core reviews the design of targeting constructs, electroporates targeting construct DNA into ES cells, and then selects and amplifies ES cell colonies in duplicate 96 well plates; one plate is cryogenically stored and the other provided to investigators to screen for homologous recombination. The Core then grows promising ES cell clones, checks karyotypes and provides the targeted ES cells to the Transgenic Facility for injection into blastocysts.

胚胎干细胞核心 CI B1。建立ES细胞核心： ES细胞核心是在该奖项的支持下成立的，并于四年前开始运作。它位于-500平方米的木材基础科学大楼9楼。英国《金融时报》与JHU SOM转基因动物设施相邻的空间。沃里博士担任胚胎干细胞核心主任，并与转基因动物设施主任罗杰·里夫斯博士密切合作，整合这些核心的功能 (见里夫斯博士(顾问)的支持信)。霍莉·惠灵顿女士担任该设施的核心经理。ES细胞核心审查靶向构建物的设计，电穿孔靶向将DNA构建到ES细胞中，然后在两个96孔板中选择和放大ES细胞克隆；一个板被低温储存，另一个板被提供给研究人员以筛选同源重组。然后，Core培养有希望的ES细胞克隆，检查核型，并向 用于注射到囊胚中的转基因设备。