Regeneration in the Adult Drosophila Brain

成年果蝇大脑的再生

• 批准号: 8807255
• 负责人: GRACE E BOEKHOFF-FALK
• 金额: $ 7.53万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8807255
• 关键词: Address; Adopted; Adult; Alzheimer' s Disease; Animal Model; Apoptosis; Area; Axon; Biological Metamorphosis; Biological Models; Birth; Brain; Brain Injuries; Brain Part; Cell Differentiation process; Cell Proliferation Regulation; Cells; Data; Dendrites; Development; Disease; Drosophila genus; Drosophila melanogaster; Future; Generations; Genes; Genetic Models; Genetic Screening; Goals; Human; Immunohistochemistry; Individual; Injury; Knowledge; Label; Learning; Maintenance; Measures; Medical; Memory; Mitotic Activity; Modeling; Mushroom Bodies; Natural regeneration; Nerve Regeneration; Nervous system structure; Neurodegenerative Disorders; Neuroglia; Neurons; Parkinson Disease; Play; Population; Process; Proliferating; Regulator Genes; Role; Safety; Series; Staging; Staining method; Stains; Stem cell transplant; Stem cells; System; Techniques; Testing; Therapeutic; United States; Work; adult neurogenesis; base; cell type; injured; lifetime risk; nerve stem cell; neural precursor cell; neurogenesis; novel; prevent; progenitor; programs; public health relevance; regenerative; relating to nervous system; research study; response to injury; senescence; small molecule; tool

DESCRIPTION (provided by applicant): Appropriate regulation of cell proliferation and differentiation is essential for normal development and maintenance of the nervous system and for neural regeneration following disease or traumatic injury. We are studying neural regeneration in the genetic model system Drosophila melanogaster where we can manipulate neural precursors in the context of an intact brain. We have focused on the adult Drosophila mushroom body, a part of the brain that plays critical roles in learning and memory. Although the neural stem cells undergo apoptosis prior to adulthood, we have shown that the mushroom body can undergo regeneration following injury. Our working hypothesis is that certain types of glial cells adopt neural precursor fates, proliferate, and give rise to both new neurons and new glial cells. The goal of the experiments proposed in this application is to identify the cells that proliferate following injury and understand the process by which they give birth to new neurons and new glial cells. Once we have accomplished the aims described in this proposal, we will be poised to undertake further analyses of Drosophila neural regeneration, including forward genetic screens for regulators of the regenerative process. Our long-term goal is to gain sufficient knowledge of the regulatory gene networks that we can activate neural regenerative programs following disease or injury.

描述（由申请方提供）：细胞增殖和分化的适当调节对于神经系统的正常发育和维持以及疾病或创伤性损伤后的神经再生至关重要。我们正在研究黑腹果蝇遗传模型系统中的神经再生，我们可以在完整大脑的背景下操纵神经前体。我们关注的是成年果蝇的蘑菇体，这是大脑中在学习和记忆中起关键作用的一部分。虽然神经干细胞在成年前经历凋亡，但我们已经证明蘑菇体在损伤后可以再生。我们的工作假设是，某些类型的神经胶质细胞采用神经前体的命运，增殖，并产生新的神经元和新的神经胶质细胞。本申请中提出的实验的目标是鉴定 在损伤后增殖，并了解它们产生新神经元和新神经胶质细胞的过程。一旦我们完成了本提案中描述的目标，我们将准备对果蝇神经再生进行进一步分析，包括再生过程调节因子的前向遗传筛选。我们的长期目标是获得足够的调控基因网络的知识，我们可以激活疾病或损伤后的神经再生程序。