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Genetic Modulation of MeHg-Induced Oxidative Stress in the Developing Brain

发育中大脑中甲基汞诱导的氧化应激的基因调节

基本信息

批准号：
8764000
负责人：
Michael Aschner
金额：
$ 28.42万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-08-03 至 2017-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8764000
关键词：
1-Phosphatidylinositol 3-Kinase Affect Alkaline Phosphatase Alleles Antioxidants Astrocytes Biochemical Biological Process Brain Cell Survival Cells Data Development Generations Genes Genetic Genetic Predisposition to Disease Genetic Screening Genetic Transcription Genomics Genotype Glutathione Disulfide Heritability Human In Vitro Inbred Mouse Inbred Strains Mice Injury Isoprostanes Lead Light Lipid Peroxidation Mammalian Cell Measurement Mediating Metal exposure Methylmercury Compounds Mitochondria Modeling Modification Molecular Mouse Strains Mus Neonatal Neuraxis Neurobiology Neurons Neurotoxins Outcome Oxidation-Reduction Oxidative Stress Pathway interactions Phenotype Phosphorylation Population Predisposition Production Protein-Serine-Threonine Kinases Protocols documentation Quantitative Trait Loci Rattus Reactive Oxygen Species Recombinants Research Resources Response Elements Role Signal Pathway Signal Transduction Small Interfering RNA Sulfhydryl Compounds System Testing Thioredoxin Toxic effect Transcriptional Activation Transfection Transgenic Mice Up-Regulation Variant biological systems combat drinking water gene environment interaction in vivo innovation kinase inhibitor knock-down nerve injury neurobehavioral neuroprotection neurotoxic neurotoxicity novel nuclear factor-erythroid 2 overexpression response trait

项目摘要

DESCRIPTION (provided by applicant): Methylmercury (MeHg) is a potent neurotoxin. We hypothesize that under conditions of MeHg-induced oxidative stress, Nrf2 coordinates the upregulation of cytoprotective genes that combat MeHg- induced oxidative injury, and that genetic and biochemical changes that negatively impact upon Nrf2 function increase MeHg's neurotoxicity. Corollaries of this hypothesis imply (i) that genetic susceptibility to MeHg-induced neurotoxicity correlates with Nrf2 expression levels and activation of downstream genes associated with antioxidant activity, and (ii) the degree of Nrf2 upregulation represents a critical determinant of cell-specific (astrocytes vs. neurons) adaptive responses to MeHg. The approach to testing these hypotheses includes biochemical and molecular characterization of Nrf2 signaling both in vivo and in vitro (primary astrocytes and neurons), and genetic correlates of neurobiological phenotypes (biochemical, morphological and neurobehavioral endpoints), taking full advantage of the unique BXD recombinant inbred (RI) mice. Specific Aim 1 will determine if MeHg exposure in cultured murine primary cerebellar astrocytes and neurons, and during development in vivo induces oxidative stress that correlates with Nrf2 transcriptional activation. Specific Aim 2 will evaluate whether the phosphatidylinositol 3-kinase (PI3K)-serine/threonine protein kinase Akt-mediated cell survival pathway is essential for Nrf2-dependent protection against MeHg. Specific Aim 3 will test the role of Nrf2 heritability in modulating MeHg susceptibility in BXD RI mouse strains. These specific aims hold the promise of delineating common initiator signals for the modulation of MeHg neuroprotection, shedding light on neurotoxic mechanisms and susceptibility associated with exposure to this metal.

描述（由申请人提供）：甲基汞（MeHg）是一种强效神经毒素。我们假设，在甲基汞诱导的氧化应激条件下，Nrf 2协调细胞保护基因的上调，对抗甲基汞诱导的氧化损伤，以及对Nrf 2功能产生负面影响的遗传和生化变化增加甲基汞的神经毒性。这一假设的推论意味着：（一）遗传易感性甲基汞诱导的神经毒性与Nrf 2的表达水平和下游基因的激活与抗氧化活性相关，（二）Nrf 2上调的程度代表细胞特异性（星形胶质细胞与神经元）适应性反应的关键决定因素甲基汞。检验这些假设的方法包括体内和体外Nrf 2信号传导的生化和分子表征（原代星形胶质细胞和神经元），以及神经生物学表型的遗传相关性（生物化学，形态学和神经行为终点），充分利用独特的BXD重组近交系（RI）小鼠。具体目标1将确定甲基汞暴露在培养的小鼠原代小脑星形胶质细胞和神经元中，以及在体内发育过程中是否会诱导与Nrf 2转录激活相关的氧化应激。具体目标2将评估磷脂酰肌醇3-激酶（PI 3 K）-丝氨酸/苏氨酸蛋白激酶Akt介导的细胞存活途径是否对Nrf 2依赖性保护甲基汞至关重要。具体目标3将测试Nrf 2遗传性在调节BXD RI小鼠品系的甲基汞易感性中的作用。这些具体的目标持有的承诺划定共同的启动信号的调制甲基汞神经保护，揭示神经毒性机制和易感性与接触这种金属。