Kinase/phosphatase-mediated Mitochondrial Restructuring in Neuroprotection

激酶/磷酸酶介导的线粒体重构在神经保护中的作用

• 批准号: 8619667
• 负责人: STEFAN STRACK
• 金额: $ 32.7万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8619667
• 关键词: A kinase anchoring protein; Address; Apoptosis; Architecture; Binding; Bioenergetics; Biological Assay; Brain; Brain region; Buffers; Calcineurin; Calcium; Cessation of life; Complex; Cyclic AMP-Dependent Protein Kinases; Cytochromes; Cytosol; Disease; Docking; Dynamin; Enzymes; Funding; Hippocampus (Brain); Injury; Ischemia; Ischemic Brain Injury; Ischemic Stroke; Knockout Mice; Lead; Maps; Mediating; Metabolic; Metabolism; Middle Cerebral Artery Occlusion; Mitochondria; Molecular; Morphology; Mus; Nerve Degeneration; Neurodegenerative Disorders; Neuroglia; Neurons; Organelles; Outer Mitochondrial Membrane; PC12 Cells; Phosphoric Monoester Hydrolases; Phosphorylation; Phosphorylation Site; Phosphotransferases; Play; Production; Property; Protein Dephosphorylation; Protein phosphatase; Proteins; Reaction; Recruitment Activity; Regulation; Reporting; Role; Safety; Severities; Signal Transduction; Signaling Molecule; Site; Staging; Stroke; Testing; improved; in vivo; in vivo Model; infancy; mutant; neuronal survival; neuroprotection; public health relevance; scaffold; stroke therapy

DESCRIPTION (provided by applicant): There is an urgent need to improve upon the safety and treatment window of current stroke therapies. Mitochondria play key roles during both early (minutes) and late (days) stages of ischemic brain injury, and are thus recognized as promising targets for neuroprotective therapy. Mitochondrial architecture, as determined by opposing fission and fusion reactions, has recently emerged as a critical determinant for survival of both neuronal and non-neuronal cells. Mitochondrial fission catalyzed by the mechanoenzyme dynamin-related protein 1 (Drp1) facilitates cytochrome C release and apoptosis. In addition, mitochondria fragment during stroke and pathological Drp1 activation occurs in neurodegenerative disorders. On the other hand, we and others have shown that fusion of mitochondria into an interconnected network has a neuroprotective effect, which may involve increased energy production, ROS and calcium sequestration, and sparing of the organelle from autophagic degradation. Despite the widely appreciated disease relevance of mitochondrial dynamics, our understanding of regulatory mechanisms controlling mitochondrial architecture is still in its infancy. In the previous funding cycle, we identified a pivotal phosphorylation site i Drp1. Conserved in all metazoans, S656 is phosphorylated by protein kinase A (PKA) to inhibit the fission enzyme, leading to unopposed fusion of mitochondria. Opposite PKA is the calcium-dependent protein phosphatase calcineurin (CaN), which dephosphorylates S656 to promote mitochondrial fragmentation. Phospho-Drp1 protects from, while dephospho-Drp1 sensitizes PC12 cells to apoptosis. We also uncovered a potent neuroprotective activity of mitochondria-localized A kinase anchoring protein 1 (AKAP1) in hippocampal neurons, which is mediated by Drp1 phosphorylation at S656 and stabilization of the mitochondrial network. Intriguingly, a PKA binding- deficient AKAP1 mutant fragmented mitochondria, suggesting that some of the signaling molecules reported to also associate with AKAP1 may oppose mitochondrial stabilization by PKA. We propose to continue with this line of inquiry in three specific aims (SA). In SA1, we will characterize AKAP1 knockout mice for changes in mitochondrial morphology, bioenergetics, Drp1 phosphorylation, and injury severity following focal ischemia. SA2 examines the role of AKAP1-interacting protein phosphatases (PP1, CaN) in mitochondrial remodeling and neuronal survival. Finally, SA3 proposes to elucidate molecular mechanisms of CaN recruitment to Drp1 in calcium-mediated mitochondrial fission and ischemic death. The proposal addresses the overall hypothesis that AKAP1 assembles a signalosome at the outer mitochondrial membrane, which integrates death and survival signals from the cytosol and from within mitochondria to restructure the organelle via reversible phosphorylation of Drp1 at S656. The proposed studies will increase our mechanistic understanding of mitochondrial fragmentation and its regulation by reversible phosphorylation in neurons, which may lead to better therapies for neurodegeneration in stroke and disease.

描述（由申请人提供）：迫切需要改善当前卒中治疗的安全性和治疗窗。线粒体在缺血性脑损伤的早期（几分钟）和晚期（几天）都起着关键作用，因此被认为是神经保护治疗的有希望的靶点。线粒体结构，由相反的裂变和融合反应，最近出现的神经元和非神经元细胞的生存的一个关键决定因素。机械酶动力蛋白相关蛋白1（Drp 1）催化的线粒体分裂促进细胞色素C释放和细胞凋亡。此外，中风期间线粒体片段和病理性Drp 1激活发生在神经退行性疾病中。另一方面，我们和其他人已经表明，线粒体融合成一个相互连接的网络具有神经保护作用，这可能涉及增加能量产生，ROS和钙螯合，并使细胞器免于自噬降解。尽管线粒体动力学与疾病的相关性得到了广泛的重视，但我们对控制线粒体结构的调控机制的理解仍处于起步阶段。在上一个资助周期中，我们确定了一个关键的磷酸化位点i Drp 1。S656在所有后生动物中都是保守的，它被蛋白激酶A（PKA）磷酸化以抑制裂变酶，导致线粒体的无对抗融合。与PKA相反的是钙依赖性蛋白磷酸酶钙调磷酸酶（CaN），它使S656去磷酸化以促进线粒体片段化。Phospho-Drp 1保护PC 12细胞免于凋亡，而Dephospho-Drp 1使PC 12细胞对凋亡敏感。我们还发现了一个强大的神经保护活性的海马神经元，这是由S656处的Drp 1磷酸化和线粒体网络的稳定介导的AKA定位的A激酶锚定蛋白1（AKAP 1）。有趣的是，PKA结合缺陷的AKAP 1突变体使线粒体片段化，这表明报道的也与AKAP 1相关的一些信号分子可能通过PKA对抗线粒体稳定。我们建议在三个具体目标（SA）上继续进行这一调查。 在SA 1中，我们将表征AKAP 1敲除小鼠的线粒体形态学、生物能量学、Drp 1磷酸化和局灶性缺血后损伤严重程度的变化。SA 2检查AKAP 1相互作用蛋白磷酸酶（PP 1，CaN）在线粒体重塑和神经元存活中的作用。最后，SA 3提出阐明钙离子介导的线粒体分裂和缺血性死亡中CaN向Drp 1募集的分子机制。该提案解决了AKAP 1在线粒体外膜组装信号体的总体假设，该信号体整合来自细胞溶质和线粒体内的死亡和存活信号，以通过S656处Drp 1的可逆磷酸化来重构细胞器。拟议的研究将增加我们对线粒体片段化及其通过神经元中可逆磷酸化调节的机制理解，这可能会导致更好的中风和疾病神经变性治疗。