Investigation into the immunogenicity of dendritic cell-derived exosomes

树突状细胞来源的外泌体的免疫原性研究

• 批准号: nhmrc : 148917
• 负责人: Prof Helen O'Neill
• 金额: $ 17.14万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2001
• 资助国家: 澳大利亚
• 起止时间: 2001-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/investigation-immunogenicity-dendritic-derived-exosomes/82951
• 关键词: Investigation; into; immunogenicity; dendritic; cell

Dendritic cells are essential in immune responses. They have unique capacity to stimulate lymphocytes specific to viruses, bacteria and cancers. They are extremely rare and difficult to isolate. We have developed a method of culture which gives a continuous supply of dendritic cells. Cells produced in our culture also produce a high yield of acellular membranous particles called 'exosomes' which have been previously been very difficult to isolate and characterise. Some preliminary reports suggest that exosomes can induce or modify immune responses and that they have enormous immunotherapeutic potential. Further study of their clinical application is limited by the difficulty of isolating enough dendritic cells from which to isolate exosomes. This study will involve production and characterisation of exosomes from our unique murine dendritic cell culture system. Exosomes isolated will be assessed in terms of potential for immunotherapeutic treatment of disease such as cancer, viral infection and autoimmunity.

树突状细胞在免疫反应中是必不可少的。它们具有刺激对病毒、细菌和癌症特异性的淋巴细胞的独特能力。它们非常罕见，很难分离。我们已经开发出一种培养方法，可以持续提供树突状细胞。在我们的培养物中产生的细胞还产生高产量的称为“外泌体”的非细胞膜颗粒，其以前非常难以分离和纯化。一些初步报告表明，外泌体可以诱导或改变免疫应答，并且它们具有巨大的免疫抑制潜力。其临床应用的进一步研究受到难以分离足够的树突状细胞以从中分离外泌体的限制。本研究将涉及从我们独特的鼠树突状细胞培养系统中生产和表征外泌体。将评估分离的外泌体用于免疫治疗疾病如癌症、病毒感染和自身免疫的潜力。