Upgrade of a FACS Aria Cell Sorter

 描述(由申请人提供)：申请资金，以便在佛蒙特州大学的流式细胞仪和细胞分选(FCCS)设施安装一台相干的50 mW 561 nm固态激光器，并升级一台FACS Aria高速细胞分选机。医学院的FCCS核心设施是目前大学里唯一的细胞分选机。因此，这一单一仪器必须能够满足该大学所有研究人员的需要，他们的研究需要细胞分类。目前，该工具支持15名NIH支持的研究人员和27名NIH赠款，并为来自不同学科的广泛调查人员社区提供服务。研究重点的转移和新教师的招聘都导致了许多研究人员需要对表达红色荧光蛋白的细胞进行分类，这些细胞最适合由561 nm的激光激发。由于我们目前的仪器没有这种激光，这些研究人员不能进行他们由NIH资助的研究。我们已经确定，我们可以通过升级我们现有的仪器来满足我们研究人员的需求，以便可以安装561 nm激光。这次升级只是购买一台新仪器成本的一小部分。升级后的仪器仍将位于FCC核心，该核心已存在20多年。该机构由一位经验丰富且经认可的科学家/细胞测量师管理，由一位在流式细胞仪领域拥有20多年经验的教师指导，并由一个内部咨询委员会监督。此外，该设施继续得到佛蒙特州大学医学院的大力机构支持。

 DESCRIPTION (provided by applicant): A request is made for funds to install a Coherent 50 mW 561 nm solid-state laser and upgrade a FACS Aria high-speed cell sorter at the Flow Cytometry and Cell Sorting (FCCS) facility at the University of Vermont. The FCCS Core facility in the College of Medicine houses the only cell sorter present at the University. Therefore, this single instrument must be able to serve the needs of all investigators at the University who require cell sorting for their research. Currently, this instrument supports 15 NIH-supported investigators and 27 NIH grants and is serving a broad community of investigators from a variety of disciplines. Both shifting emphases in research and the recruitment of new faculty have resulted in a number of investigators who need to sort cells expressing red fluorescent proteins, which are optimally excited by a 561 nm laser line. Since our current instrument does not possess this laser, these investigators cannot conduct their NIH-funded research. We have determined that we can meet the needs of our investigators by upgrading our current instrument so that a 561 nm laser can be installed. This upgrade is a fraction of the cost of the purchase of a new instrument. The upgraded instrument will remain located in the FCCS core, which has been in existence for over 20 years. This facility is managed by an experienced and accredited scientist/cytometrist, is directed by a faculty advisor with over 20 years of experience in flow cytometry, and is overseen by an internal advisory committee. In addition, the facility continues to enjoy strong institutional support from the University Of Vermont College Of Medicine.