Sample Engineering Core

样本工程核心的功能是推进预分析策略和方法，包括 样品采集、现场前处理和样品运输。核心将利用以下方面的专业知识 微流控设计、快速成型、制造工艺工程和生物检测开发 为三个项目中的每个项目创建设备和协议。核心将密切协调地执行任务 通过这三个项目确保在链接到自动化高通量分析时有准确的终端 仪器越来越多地出现在大学、工业和临床检测实验室中。 样品工程核心的第一个目标是开发一种半自动的设备，用于采集和前处理 血样加工，适用于所有项目。该装置的原型将被制造出来 采用先进的3D打印技术。 第二个目标是开发现场前处理技术，以稳定运输中的血液样本 同时开始分析过程。一种稳定和运输样品以供使用的方法 项目1和2的终点将使用预装试剂的真空密封管。以纸张为基础的 将开发用于项目3终端的稳定卡。 最终目标是开发一种廉价、轻便、由电池供电的运输集装箱，能够精确地 运输至集中分析设施期间的温度控制。这一发展将使 项目1的微核试验培养在样本采集后立即开始，因此利用 以前被浪费的发货时间。最终的结果将是更快地得出最终的生物剂量学结果。

The function of the Sample Engineering Core is to advance pre-analysis strategies and methods including sample collection, in-field preprocessing, and transportation of samples. The Core will leverage expertise in microfluidic design, rapid prototyping, manufacturing process engineering, and biological assay development to create devices and protocols for each of the three Projects. The Core will perform tasks in close coordination with the three Projects to ensure accurate endpoints when linking to the automated high-throughput analysis instruments, which are becoming increasingly available in university, industry, and clinical testing laboratories. The first aim of the Sample Engineering Core is to develop a semi-automated device for collection and pre- processing of blood samples, applicable to all of the Projects. Prototypes of the device will be manufactured using advanced 3D printing technology. The second aim is to develop in-field pre-processing technologies to stabilize blood samples for transportation while concurrently beginning the analysis process. A method of stabilizing and shipping samples for use with Project 1 and 2 endpoints will utilize vacuum-sealed tubes preloaded with reagents. A paper-based stabilization card will be developed to use with Project 3 endpoints. The final aim is to develop an inexpensive, lightweight, battery-powered shipping container capable of precise thermal control during transportation to centralized analysis facilities. This development will allow the micronucleus assay culture for Project 1 to begin immediately following sample acquisition, therefore utilizing shipping time which was formerly wasted. The end result will be faster times to final biodosimetric results.