Sample Engineering Core

样本工程核心的功能是推进预分析策略和方法，包括 样品采集、现场预处理和样品运输。核心将利用专业知识， 微流控设计、快速成型、制造工艺工程和生物测定开发， 为三个项目中的每一个创建设备和协议。核心将密切协调执行任务 与三个项目，以确保准确的终点时，连接到自动化的高通量分析 仪器，这是越来越多地在大学，工业和临床测试实验室。 样品工程核心的第一个目标是开发一种半自动化的收集和预处理设备， 血液样本的处理，适用于所有项目。将制造器械原型 使用先进的3D打印技术。 第二个目标是开发现场预处理技术，以稳定血样，便于运输 同时开始分析过程。一种稳定和运输样品以供使用的方法， 项目1和2终点将使用预装试剂的真空密封管。纸质 将开发稳定卡，用于项目3终点。 最终的目标是开发一种便宜，轻便，电池供电的集装箱，能够精确地 运输至集中分析设施期间的热控制。这一发展将使 项目1的微核试验培养在样本采集后立即开始，因此使用 以前浪费的运输时间。最终结果将是获得最终生物剂量测定结果的更快时间。

The function of the Sample Engineering Core is to advance pre-analysis strategies and methods including sample collection, in-field preprocessing, and transportation of samples. The Core will leverage expertise in microfluidic design, rapid prototyping, manufacturing process engineering, and biological assay development to create devices and protocols for each of the three Projects. The Core will perform tasks in close coordination with the three Projects to ensure accurate endpoints when linking to the automated high-throughput analysis instruments, which are becoming increasingly available in university, industry, and clinical testing laboratories. The first aim of the Sample Engineering Core is to develop a semi-automated device for collection and pre- processing of blood samples, applicable to all of the Projects. Prototypes of the device will be manufactured using advanced 3D printing technology. The second aim is to develop in-field pre-processing technologies to stabilize blood samples for transportation while concurrently beginning the analysis process. A method of stabilizing and shipping samples for use with Project 1 and 2 endpoints will utilize vacuum-sealed tubes preloaded with reagents. A paper-based stabilization card will be developed to use with Project 3 endpoints. The final aim is to develop an inexpensive, lightweight, battery-powered shipping container capable of precise thermal control during transportation to centralized analysis facilities. This development will allow the micronucleus assay culture for Project 1 to begin immediately following sample acquisition, therefore utilizing shipping time which was formerly wasted. The end result will be faster times to final biodosimetric results.