FACS Analysis and Cell Sorting

FACS 分析和细胞分选

• 批准号: 8661264
• 负责人: DOUGLAS W LOSORDO
• 金额: $ 21.18万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8661264
• 关键词: Animal Model; Animals; Antibodies; Apoptosis; Apoptotic; Biological Assay; Cardiovascular Diseases; Cardiovascular system; Cell Cycle; Cell Extracts; Cell Separation; Cell surface; Cells; Color; Computer software; Data Analyses; Educational process of instructing; Factor Analysis; Flow Cytometry; Funding; Goals; Histology; Human; Individual; Laboratories; Leukocytes; Medicine; Mus; Operative Surgical Procedures; Patients; Phosphoproteins; Population; Proteins; Protocols documentation; Regenerative Medicine; Research; Research Personnel; Resources; Running; Sampling; Serum; Specimen; Staining method; Stains; Technology; Training; Universities; base; cell analyzer; cost; cytokine; member; molecular imaging; progenitor; programs; screening; transcription factor

The FACS Analysis and Cell Sorting Core will perform all assays on specimens from patients and mice that relate to flow cytometry including analysis and cell sorting and Luminex-based studies. Since non-cancer investigators from Northwestern University have limited access to the FACSAria and studies that require substantial cell sorting are cost-prohibitive, the newly established FACS Analysis and Cell Sorting Core will markedly expand the capabilities of the PPG investigators by providing antibody cocktails, protocols, and expertise that will enhance and facilitate the currently funded research and potentially new studies.

FACS分析和细胞分选核心将执行 与流式细胞术相关的患者和小鼠标本的所有测定，包括分析和细胞分选 和基于Luminex的研究。由于来自西北大学的非癌症研究人员 FACSAria和研究，需要大量的细胞分选是成本高昂的，新成立的 FACS分析和细胞分选核心将显著扩展PPG研究者的能力， 提供抗体鸡尾酒，协议和专业知识，将加强和促进目前资助的 研究和潜在的新研究。