Phase variable control of Acinetobacter baumannii virulence

鲍曼不动杆菌毒力的相变量控制

• 批准号: 8809155
• 负责人: Philip N. Rather
• 金额: $ 19.12万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-01 至 2016-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8809155
• 关键词: Acinetobacter baumannii; Address; Antibiotics; Binding; Cell Shape; Cells; Cellular Morphology; Clinical; Defect; Development; Event; Exhibits; Feedback; Frequencies; Gene Expression; Genes; Genetic; Goals; Gram-Negative Bacteria; Human; In Vitro; Infection; Mediating; Molecular; Molecular Profiling; Morphology; Mutation; Nosocomial Infections; Phase; Phenotype; Production; RNA; Regulation; Research Personnel; Signal Transduction; Surface; Testing; Variant; Virulence; Work; base; cell motility; combat; genome sequencing; in vivo; novel; public health relevance; quorum sensing; resistant strain; response

 DESCRIPTION (provided by applicant): The Gram-negative bacterium Acinetobacter baumannii is a leading cause of nosocomial infections in humans. The emergence of A. baumannii strains resistant to most, and in some cases, all available antibiotics has made the treatment of these infections exceedingly difficult. This underlies the importance of finding new ways to combat these infections. This proposal addresses a phase variable mechanism in A. baumannii that controls multiple phenotypes, including colony opacity, quorum sensing and virulence. Using a combination of allelic replacement and whole genome sequencing, the mechanism responsible for this phase variation will be determined. The impact of phase variation on global gene expression will also be determined using RNA- Seq. Ultimately, an understanding of the molecular mechanisms that control this phase variation and the overall phenotypes impacted by this variation will provide important information on the regulation of virulence in A. baumannii.

 描述（由申请方提供）：革兰氏阴性菌鲍曼不动杆菌是人类医院感染的主要原因。A.鲍曼不动杆菌菌株对大多数（在某些情况下是所有可用的抗生素）具有耐药性，使得这些感染的治疗变得极其困难。这突出了寻找新方法来对抗这些感染的重要性。该建议提出了一种相位可变机构在A。鲍曼不动杆菌控制多种表型，包括菌落不透明性、群体感应和毒力。使用等位基因替换和全基因组测序的组合，将确定负责该相位变化的机制。还将使用RNA-Seq确定相位变化对全局基因表达的影响。最终，了解控制这种阶段变化的分子机制和受这种变化影响的整体表型将提供重要的信息，在A.鲍曼不动杆菌。