Cell and Immunohistochemistry Core

在过去的6年里，我们继续协调羊血管和人脐带的分布，并分离、纯化和表征UAEC和HUVEC-CS细胞，用于所有四个项目。所有这些细胞现在都有了很好的特征。最近，我们还建立了原代HUVE和huec，所有原代人内皮细胞都保留了许多正常的体内生理功能，例如对生长因子的反应和负责血管扩张剂生成（NO）的关键基因的表达，这些是我们将在本项目资助中确定的细胞反应。Core C冷冻了许多瓶UAEC，

During the last 6 years we have continued to coordinate distribution of ovine vessels and human umbilical cords, and isolate, purify, and characterize UAEC and HUVEC-CS cells for usage of all four projects. All of these cells are now very well characterized. Recently, we have also established primary HUVE and HUAEC, All primary human endothelial cells retain many normal in vivo physiologic functions such as in responses to growth factors and expression of key genes responsible for vasodilator production (NO), which are the cell responses we will determine in this Program Project Grant. Core C has frozen down many vials of UAEC, HUVEC, and HUAEC stocks for usage of the current application. We have also established standard immunocytochemistry protocols for colorimetric and fluorescent microscopy analysis, and transmission electron microscopy (TEM) analysis for Gap junction ultra-structural morphology. Thus, by acting as a Core, we will provide essential cell supplies, much needed technical expertise in NO detection and immunocytochemistry, TEM, and quality assurance, so contributing to the ability to directly compare data from different projects and between cell types.