Molecular Detection of DNA Hydroxymethylation for Cancer Screening

用于癌症筛查的 DNA 羟甲基化分子检测

• 批准号: 8851166
• 负责人: Adam Roger Hall
• 金额: $ 13.48万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8851166
• 关键词: Binding; Biological Assay; Biological Markers; Biotin; Breast Carcinoma; Charge; Chicago; Clinical; DNA; DNA Modification Process; Detection; Diagnostic; Discrimination; Disease; Early Diagnosis; Epigenetic Process; Film; Gene Targeting; Genes; Genetic Materials; Genomic DNA; Genomics; Goals; Individual; Label; Malignant Neoplasms; Mammary Gland Parenchyma; Mammary Neoplasms; Measurement; Measures; Membrane; Methods; Modeling; Modification; Molecular; Normal tissue morphology; Oligonucleotides; Patients; Public Health; Research; Research Personnel; Role; Sample Size; Sampling; Screening for cancer; Sensitivity and Specificity; Site; Solutions; Specificity; Speed; Staging; Streptavidin; System; Techniques; Technology; Tissue Sample; Tissues; Tumor Suppressor Genes; Tumor Suppressor Proteins; Tumor Tissue; Tumorigenicity; Variant; cancer type; comparative; density; direct application; epigenetic variation; innovation; nanopore; nanoscale; novel; prognostic tool; public health relevance; research study; screening; single molecule; solid state; tool; tumor; tumor initiation

 DESCRIPTION (provided by applicant): This project will develop a strategy to detect levels of hydroxymethylation (hmC) in genomic DNA. We will first combine a method to selectively attach a biotin to hmC with our demonstrated technique of detecting single biotin modifications in DNA to demonstrate the efficacy of our approach and optimize experimental conditions. We will then apply the technique to clinical tissue samples. First, we will measure the global hmC content of normal breast tissue and of breast carcinoma and compare our findings with results from conventional approaches. Finally, we will utilize the capability of our approach to isolate modified DNA selectively and apply the system to identifying alterations in the hmC content of a fragment of the LZTS1 gene, an important tumor suppressor.

 描述（由申请人提供）：本项目将开发一种检测基因组DNA中羟甲基化（hmC）水平的策略。我们将首先结合联合收割机的方法，选择性地将生物素连接到hmC与我们证明的技术，检测DNA中的单一生物素修饰，以证明我们的方法的有效性和优化实验条件。然后，我们将该技术应用于临床组织样本。首先，我们将测量正常乳腺组织和乳腺癌的hmC含量，并将我们的发现与传统方法的结果进行比较。最后，我们将利用我们的方法选择性地分离修饰的DNA的能力，并将该系统应用于识别LZTS1基因片段（一种重要的肿瘤抑制基因）的hmC含量的改变。