Microsurgery and Histopathology Core

显微外科和组织病理学核心

• 批准号: 8934953
• 负责人: Zheng J Zhang
• 金额: $ 48.27万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8934953
• 关键词: Address; Advisory Committees; Allografting; Animal Model; Animals; Archives; Area; Biological Preservation; Blood specimen; Caring; Cells; Charge; Clinical; Collaborations; Communities; Complex; Core Facility; Cytomegalovirus; Data; Development; Ensure; Epigenetic Process; Evaluation; Goals; Graft Rejection; Heart Transplantation; Histologic; Histological Techniques; Histology; Histopathology; Immunofluorescence Immunologic; Immunofluorescence Microscopy; Immunohistochemistry; Individual; Infection; Inflammation Mediators; Injury; Investigation; Kidney; Kidney Transplantation; Lead; Methodology; Microsurgery; Modeling; Molecular; Monitor; Mus; Operative Surgical Procedures; Organ Harvestings; Organ Transplantation; Pathologist; Pathology; Pathway interactions; Pharmaceutical Preparations; Positioning Attribute; Procedures; Process; Renal function; Research; Research Design; Research Personnel; Resources; Rodent; Sampling; Services; Site; Species Specificity; Staining method; Stains; Supervision; Surgical Models; Survival Rate; Techniques; Testing; Therapeutic Intervention; Tissue Banks; Tissue Sample; Tissues; Transplant Recipients; Transplantation; Universities; Work; allograft rejection; animal care; experience; flexibility; in vivo; insight; interest; light microscopy; meetings; mouse development; mouse model; novel strategies; prevent; programs; research study; sample collection; sample fixation; skills; success

PROJECT SUMMARY - Microsurgery and Histopathology Core Mouse transplant models are essential for the in vivo studies exploring molecular mechanisms of CMV reactivation following organ transplantation as proposed in this program project. The Microsurgery and Histopathology core (Core A) will serve as a central resource with state-of-the-art microsurgical techniques and histopathological methodologies for the studies proposed in the program projects to help the project leaders achieve their research goals. Core A will providing the necessary skill sets and expertise to allow the use of mouse transplant models with guaranteed technical success and efficiencies. Specifically, Core A will be responsible for performing all mouse transplant surgical procedures, post-transplant care, functional monitoring, and tissue sample collection and processing under Dr. Zhang's direction. Core A will perform histopathological analysis to characterize of allograft injuries (e.g. rejection) on the tissues generated from the Program Project by using state-of-the-art techniques involving histology, immunohistochemistry and immunofluorescence staining with Dr. Kanwar's supervision. Dr. Zhang is the director of the Microsurgery Core at the Northwestern University Comprehensive Transplant Center (NUCTC), a core that has served all interested investigators in the Northwestern University community for more than 15 years with more than 4000 mouse transplant procedures. Dr. Kanwar is an established pathologist with a longstanding track record as an independent investigator in areas of kidney injury has provided important input into our mouse transplant experiments over the past two decades. Both Dr. Zhang and Dr. Kanwar have had a long term and extensive collaboration with the leaders of each project. Core A will be staffed by experienced microsurgeons and lab technologists who have significant experience either in performing rodent vascularized organ transplants or in state-of-the-art histological techniques. It is therefore equipped to perform all the mouse transplant procedures and histopathologic techniques requested by this program project. Furthermore, Core A will be in charge of sample submission to Core B or relevant off-site collaborators for further analyses as needed. It will ensure maximum utilization of the tissue samples, and will facilitate interactions between various projects and between project PIs. Both Dr. Zhang and Dr. Kanwar will participate in study design and data interpretation. They will provide intellectual input specific to how various models can be used to derive mechanistic insights and/or potential therapeutic interventions and will use their expertise to guide the development of novel approaches as desired by the project leaders.

项目总结-显微外科和组织学核心 小鼠移植模型是探索CMV分子机制的体内研究所必需的 器官移植后的再激活，如本计划项目所提出的。显微外科和 组织学核心（核心A）将作为具有最先进显微外科技术的中心资源， 组织病理学方法的研究中提出的计划项目，以帮助项目负责人 实现他们的研究目标。核心A将提供必要的技能和专业知识， 小鼠移植模型，保证技术成功和效率。核心A将是 负责执行所有小鼠移植手术程序，移植后护理，功能 在张博士的指导下进行监测和组织样本收集和处理。核心A将执行 组织病理学分析，以表征由移植物产生的组织上的同种异体移植物损伤（例如排斥反应）。 利用最先进的技术，包括组织学、免疫组织化学和 在Kanwar博士的监督下进行免疫荧光染色。张医生是显微外科中心主任 在西北大学综合移植中心（NUCTC），一个核心，已服务于所有 感兴趣的研究人员在西北大学社区超过15年，超过4000 小鼠移植程序。Kanwar博士是一位公认的病理学家， 肾损伤领域的独立研究者为我们的小鼠移植提供了重要的投入 在过去的二十年里，张博士和Kanwar博士都有一个长期和广泛的 与每个项目的负责人合作。核心A将由经验丰富的显微外科医生和实验室 在啮齿动物血管化器官移植或 最先进的组织学技术因此，它可以执行所有的小鼠移植程序 和组织病理学技术。此外，核心A将负责 根据需要，向核心B或相关场外合作者提交样本以进行进一步分析。它将确保 最大限度地利用组织样本，并将促进各种项目之间的互动， 项目PI。Zhang博士和Kanwar博士将参与研究设计和数据解释。他们将 提供关于如何使用各种模型来获得机械见解的知识输入，和/或 潜在的治疗干预措施，并将利用他们的专业知识来指导新方法的开发 如项目领导人所愿。