CORE A: CELL BIOLOGY CORE

在过去的供资周期中，建立了细胞生物学核心提供的服务 促进了与核心目标相关的各种研究。与肿瘤相关的细胞生物学研究 DRTC已经在研究社区内扩大了规模，这一点从出版物和赠款中得到了证明 来自传统上不在糖尿病和新陈代谢领域工作的研究人员的申请。 DRTC的脂肪组织库和脂肪生物学专业知识和服务被移交运营 在新资助的大西洋中部营养和肥胖研究中心(NORC)的赞助下 马里兰大学。这确保了脂肪组织相关服务的加强和连续性。 DRTC社区，同时释放用于创新和扩展服务的资源 通过巴尔的摩DRTC的细胞生物学核心。细胞生物学核心启动了一个本地存储库 组织/细胞特异性Cre-deleter或氧化环丙氨酸可调控转基因小鼠模型的建立 这些特征良好的小鼠，在C57BI/6背景下，已经成为 研究社区。回应调查人员的需求以及与以下方面有关的问题 关于新陈代谢和糖尿病，细胞生物学已经扩大了它在以下领域的服务和专业知识 细胞生物能量学和线粒体生物学。这一新增加的项目将提供专业知识和服务 对线粒体内容、线粒体形状和星座进行成像和量化，以评估 线粒体动力学(融合和分裂)，提供量化线粒体基因和 蛋白质表达(如解偶联蛋白、电子转移链复合体)和细胞 线粒体DNA含量。此外，DRC用户将获得独特的遗传小鼠模型(例如 FLOXED DRP1，FLOXECI Opal小鼠)，它允许有条件地组织特异性地消融参与 线粒体的融合和分裂，以及在氧化细胞应激反应中介导细胞凋亡。

During the past funding cycle, the establishment of the services provided by the Cell Biology Core has fostered a large variety of research related to the Core Objectives. Cell Biological Studies related to the DRTC have expanded within the research community as evidenced by publications as well as grant applications from investigatoVs who traditionally do not work in the area of diabetes mellitus and metabolism. The adipose tissue bank and adipose biology expertise and service of the DRTC was transferred to operate under the auspices of the newly funded Mid-Atlantic nutrition and obesity research center (NORC) at the University of Maryland. This ensured a strengthening and continuity of adipose tissue related services to the DRTC community while freeing resources, which were used for innovation and expansion of services through the Cell Biology Core of the Baltimore DRTC. The Cell Biology Core started a local repository of transgenic mouse models of tissue/cell specific transgenic CRE -deleter ordoxycylcine regulatable mice. These well-characterized mice, in the C57BI/6 background, have become a valuable resource for the research community. Responding to investigators' needs as well as to pertinent questions related to metabolism and diabetes mellitus, the Cell Biology has expanded its services and expertise in the area of cellular bioenergetics and mitochondrial biology. This new addition will provide expertise and services to image and quantify mitochondrial content and mitochondrial shape and constellation, to assess mitochondrial dynamics (fusion and division), provide expertise in quantification of mitochondrial gene and protein expression (e.g. uncoupling proteins, complexes of the electron transfer chain) and cellular mitochondrial DNA content. In addition DRC users will gain access to unique genetic mouse models (e.g. floxed Drp1, floxeci Opal mice), which allow conditional tissue specific ablation of proteins involved in mitochondrial fusion and division and in mediating apoptosis in response to oxidative cellular stress.