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Innate immunity-associated recycling endosome in Arabidopsis and pathogen attack

拟南芥中先天免疫相关的回收内体和病原体攻击

基本信息

批准号：
8814735
负责人：
SHENG YANG HE
金额：
$ 28.69万
依托单位：
MICHIGAN STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-01-01 至 2018-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8814735
关键词：
ADP-Ribosylation Factors Arabidopsis Arabidopsis Proteins Bacteria Bacterial Infections Basic Science Biochemistry Biological Response Modifiers Cell membrane Cells Cellular biology Communicable Diseases Confocal Microscopy Development Disease Early Endosome Endosomes Escherichia coli EHEC Eukaryotic Cell Family Goals Grant Guanine Nucleotide Exchange Factors Hereditary Disease Human Human Genetics Immune Immune System Diseases Immune response Immune system Immunity Infection Intervention Knowledge Laboratories Mammals Mediating Membrane Proteins Methods Michigan Modeling Molecular Genetics Natural Immunity Nature Paper Pathogenesis Plants Principal Investigator Proteins Protocols documentation Pseudomonas syringae Receptor Signaling Recycling Reporting Research Role Signal Pathway Signal Transduction Site System Transcript Ubiquitination Universities Vesicle Yang base combat extracellular insight killings microbial multicatalytic endopeptidase complex mutant novel pathogen protein complex public health relevance research study spatiotemporal trafficking

项目摘要

DESCRIPTION (provided by applicant): Innate immunity-associated endosomes and pathogen attack in Arabidopsis PI: HE, Sheng Yang; Michigan State University Project summary The long-term goal of this research is to elucidate the vesicle trafficking network of the innate immune system and how pathogens modulate this network to cause infectious diseases. Plants and mammals share functionally analogous innate immune systems that are important for combating microbial pathogens. Despite many exciting advances over the past two decades in our understanding of plant and mammalian innate immune systems, major knowledge gaps remain in both systems. A particularly poorly understood aspect of the innate immune system is the vesicle trafficking component that controls immunity-associated receptors, signaling components, and cargoes. For the past two decades, the tractable Arabidopsis-Pseudomonas syringae pathosystem has been used to discover and characterize many innate immune regulators, as well as pathogen effectors that modulate innate immune responses. By studying the bacterial effector HopM1, the principal investigator's laboratory discovered the MIN7 protein, an Arabidopsis guanine nucleotide exchange factor (GEF) belonging to the ADP ribosylation factor (ARF) family. MIN7 is located in early endosomes that recycle plasma membrane proteins and is required for all major branches of plant innate immunity, suggesting a key role in innate immune traffic. The identification of MIN7-associated endosomes now provides an exciting entry point for gaining a comprehensive understanding of the poorly characterized recycling endosomes in immune traffic in a model eukaryotic system. In this research, an integrative approach, involving methods in molecular genetics, cell biology, biochemistry, and microbial pathogenesis, will be taken to understand MIN7-associated vesicle traffic. The specific goals of this project are: 1) to investigate MIN7 protein stability during disease and immunity, 2) to identify and characterize the components of the immune-associated MIN7 protein complex and MIN7-associated recycling endosomes, and 3) to characterize the newly discovered MIN7-associated focal immune zones (MAIZs). Elucidating the mechanisms by which MIN7 regulates innate immune traffic has the potential to illuminate the fundamental principles underlying innate immune responses. Enhanced understanding of host innate immune systems and their manipulation by microbial pathogens promises to provide fundamental knowledge for the development of novel methods of disease intervention in humans and plants.