Innate immunity-associated recycling endosome in Arabidopsis and pathogen attack

拟南芥中先天免疫相关的回收内体和病原体攻击

• 批准号: 8990976
• 负责人: SHENG YANG HE
• 金额: $ 28.67万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8990976
• 关键词: ADP-Ribosylation Factors; Arabidopsis; Arabidopsis Proteins; Bacteria; Bacterial Infections; Basic Science; Biochemistry; Biological Response Modifiers; Cell membrane; Cells; Cellular biology; Communicable Diseases; Confocal Microscopy; Development; Disease; Early Endosome; Endosomes; Escherichia coli EHEC; Escherichia coli O157:H7; Eukaryotic Cell; Family; Goals; Grant; Guanine Nucleotide Exchange Factors; Health; Hereditary Disease; Human; Human Genetics; Immune; Immune System Diseases; Immune response; Immune system; Immunity; Infection; Intervention; Knowledge; Laboratories; Mammals; Mediating; Membrane Proteins; Methods; Michigan; Modeling; Molecular Genetics; Natural Immunity; Nature; Paper; Pathogenesis; Plants; Principal Investigator; Proteins; Protocols documentation; Pseudomonas syringae; Receptor Signaling; Recycling; Reporting; Research; Role; Signal Pathway; Signal Transduction; Site; System; Transcript; Ubiquitination; Universities; Vesicle; Yang; base; combat; extracellular; insight; killings; microbial; multicatalytic endopeptidase complex; mutant; novel; pathogen; protein complex; research study; spatiotemporal; trafficking

 DESCRIPTION (provided by applicant): Innate immunity-associated endosomes and pathogen attack in Arabidopsis PI: HE, Sheng Yang; Michigan State University Project summary The long-term goal of this research is to elucidate the vesicle trafficking network of the innate immune system and how pathogens modulate this network to cause infectious diseases. Plants and mammals share functionally analogous innate immune systems that are important for combating microbial pathogens. Despite many exciting advances over the past two decades in our understanding of plant and mammalian innate immune systems, major knowledge gaps remain in both systems. A particularly poorly understood aspect of the innate immune system is the vesicle trafficking component that controls immunity-associated receptors, signaling components, and cargoes. For the past two decades, the tractable Arabidopsis-Pseudomonas syringae pathosystem has been used to discover and characterize many innate immune regulators, as well as pathogen effectors that modulate innate immune responses. By studying the bacterial effector HopM1, the principal investigator's laboratory discovered the MIN7 protein, an Arabidopsis guanine nucleotide exchange factor (GEF) belonging to the ADP ribosylation factor (ARF) family. MIN7 is located in early endosomes that recycle plasma membrane proteins and is required for all major branches of plant innate immunity, suggesting a key role in innate immune traffic. The identification of MIN7-associated endosomes now provides an exciting entry point for gaining a comprehensive understanding of the poorly characterized recycling endosomes in immune traffic in a model eukaryotic system. In this research, an integrative approach, involving methods in molecular genetics, cell biology, biochemistry, and microbial pathogenesis, will be taken to understand MIN7-associated vesicle traffic. The specific goals of this project are: 1) to investigate MIN7 protein stability during disease and immunity, 2) to identify and characterize the components of the immune-associated MIN7 protein complex and MIN7-associated recycling endosomes, and 3) to characterize the newly discovered MIN7-associated focal immune zones (MAIZs). Elucidating the mechanisms by which MIN7 regulates innate immune traffic has the potential to illuminate the fundamental principles underlying innate immune responses. Enhanced understanding of host innate immune systems and their manipulation by microbial pathogens promises to provide fundamental knowledge for the development of novel methods of disease intervention in humans and plants.

 描述（申请人提供）：拟南芥中先天免疫相关的内体和病原体攻击 PI：HE，Sheng Yang；密歇根州立大学项目摘要这项研究的长期目标是阐明先天免疫系统的囊泡运输网络以及病原体如何调节该网络以引起传染病。植物和哺乳动物具有功能相似的先天免疫系统，这对于对抗微生物病原体非常重要。尽管过去二十年来我们对植物和哺乳动物先天免疫系统的理解取得了许多令人兴奋的进展，但这两个系统仍然存在重大知识差距。先天免疫系统的一个特别少为人知的方面是控制免疫相关受体、信号传导成分和货物的囊泡运输成分。在过去的二十年中，易处理的拟南芥-丁香假单胞菌病理系统已被用来发现和表征许多先天免疫调节因子，以及调节先天免疫反应的病原体效应子。通过研究细菌效应子 HopM1，首席研究员的实验室发现了 MIN7 蛋白，这是一种属于 ADP 核糖基化因子 (ARF) 家族的拟南芥鸟嘌呤核苷酸交换因子 (GEF)。 MIN7 位于回收质膜蛋白的早期内体中，是植物先天免疫所有主要分支所必需的，这表明在先天免疫运输中发挥着关键作用。 MIN7 相关内体的鉴定现在为全面了解模型真核系统免疫运输中特征较差的回收内体提供了一个令人兴奋的切入点。在这项研究中，将采用涉及分子遗传学、细胞生物学、生物化学和微生物发病机制等方法的综合方法来了解 MIN7 相关的囊泡运输。该项目的具体目标是：1) 研究疾病和免疫过程中 MIN7 蛋白的稳定性，2) 鉴定和表征免疫相关 MIN7 蛋白复合物和 MIN7 相关回收内体的成分，以及 3) 表征新发现的 MIN7 相关局灶性免疫区 (MAIZ)。阐明 MIN7 调节先天免疫流量的机制有可能阐明先天免疫反应的基本原理。增强对宿主先天免疫系统及其微生物病原体操纵的理解有望为人类和植物疾病干预新方法的开发提供基础知识。