Genotyping and DNA/RNA Analysis

基因分型和 DNA/RNA 分析

• 批准号: 8681197
• 负责人: DANIELLE Renee REED
• 金额: $ 13.07万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8681197
• 关键词: Address; Algorithms; Alleles; Animals; Area; Base Sequence; Behavior; Binding; Biology; Chemicals; Complementary DNA; Complex; Copy Number Polymorphism; DNA; DNA Sequence; DNA analysis; Data; Data Analyses; Dinucleotide Repeats; Discipline; Discrimination; Dyes; Effectiveness; Electrophoresis; Engineering; Ensure; Equipment; Fostering; Funding; Gel; Genetic Polymorphism; Genetic Variation; Genome; Genotype; Goals; Histocompatibility Testing; Housing; Human; Imagery; Individual; Institutes; Knock-out; Knowledge; Label; Laboratories; Laboratory Study; Light; Measurement; Measures; Messenger RNA; Methods; Microsatellite Repeats; Minor; Mission; Molecular Biology; Mus; National Institute on Deafness and Other Communication Disorders; Persons; Physiology; Procedures; Proteins; Psychophysics; Public Health; RNA; RNA analysis; Rattus; Relative (related person); Research; Research Methodology; Research Personnel; Research Project Grants; Resources; Scientific Advances and Accomplishments; Sensory; Sensory Physiology; Sepharose; Services; Spectrophotometry; Structure; Students; System; Taste Blindness; Teaching Method; Techniques; Time; Tissues; Training; Transgenic Mice; United States National Institutes of Health; Variant; Work; base; cell type; college; dalton; design; ds-DNA; experience; flexibility; high school; insertion/deletion mutation; instrument; instrumentation; interest; meetings; polyacrylamide; postdoctoral investigator; programs; prototype; repaired; research study

The primary expertise of Monell investigators spans a wide range of disciplines, including human sensory physiology and psychophysics (Drs. Breslin, Cowart, Dalton, Lundstrom, Pelchat, Mennella, Wysocki. Yamazaki. and Zhao), mouse and/or rat genefics (Drs. Bachmanov, Reed, and Tordoff), molecular biology (Drs. Brand, Margolskee, and Huang), and animal chemosensory physiology (Drs. Beauchamp, Lowe, Margolskee, and Reisert). Most or all of these research programs use DNA or RNA analysis methods at various fimes, but these methods often lie outside their area of expertise. This Core will serve to remove barriers from the invesfigators' use of these techniques. For instance, transgenic mice used by laboratories that study mouse behavior and physiology need to be genotyped to ensure that they are of the correct group (e.g., knockout or wild-type), but the invesfigator has no training in genotyping methods. Therefore, it is useful for the investigator to have a technician available who has been rigorously trained by experts to perform this work, to make use ofthe equipment available in the Core. Thus, not all people who study genefically engineered mice need to have all the equipment to genotype mice in their own laboratories. Likewise, invesfigators doing human sensory work might like to know their subject's genotype for key sensory polymorphisms (e.g., is the person homozygous for certain alleles associated with bitter taste blindness?). Again, this is often outside the expertise of their laboratory, but by working with this Core they can receive appropriate training and readily collect these data. Thus, this Core will provide a centralized common resource and reservoir of experience in genotyping and DNA/RNA analysis that would be inefficient to replicate in each individual laboratory. The availability of Core services extends the capabilifies of the participafing laboratories beyond what each could do individually.

蒙乃尔的主要专长 研究人员跨越了广泛的学科，包括人类感官生理学和心理物理学（Drs。 布雷斯林，考沃特，道尔顿，伦德斯特罗姆，佩尔查特，门内拉，威索基。山崎和Zhao）、小鼠和/或大鼠 遗传学（Bachmanov博士，Reed和Tornard），分子生物学（Brand博士，Margolskee和Huang），以及 动物化学感觉生理学（Beauchamp，Lowe，Margolskee和Reisert博士）。大多数或所有这些 研究计划在不同的时间使用DNA或RNA分析方法，但这些方法往往是外部的， 他们的专业领域。这一核心将有助于消除投资者使用这些技术的障碍。 例如，研究小鼠行为和生理学的实验室使用的转基因小鼠需要 进行基因分型以确保它们属于正确的组（例如，敲除或野生型），但研究者没有 基因分型方法培训。因此，研究者有一名技术人员是有用的， 已经接受过专家的严格培训，以执行这项工作， 核心因此，并非所有研究基因工程小鼠的人都需要拥有所有设备来进行基因分型 老鼠在自己的实验室。同样，从事人类感觉工作的研究者可能想知道他们的受试者的关键感觉多态性的基因型（例如，是与苦味盲症相关的某些等位基因的纯合子？）。同样，这通常超出了他们实验室的专业知识范围，但通过与该核心合作，他们可以接受适当的培训并随时收集这些数据。因此，该核心将提供基因分型和DNA/RNA分析方面的集中的共同资源和经验库， 在每个单独的实验室中重复是低效的。核心服务的可用性扩展了参与实验室的能力，超出了每个实验室单独做的事情。