Dual-Mode Plasmonic Biosensors using Bioenabled Nanomaterials

使用生物纳米材料的双模式等离子体生物传感器

• 批准号: 8886469
• 负责人: Alan X Wang
• 金额: $ 7.32万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-15 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8886469
• 关键词: 3-Dimensional; Address; Adopted; Air; Algae; Antibodies; Antigens; Binding; Biosensing Techniques; Biosensor; Cells; Chemicals; Coupling; Detection; Developing Countries; Devices; Diagnosis; Diagnostic; Diatoms; Disease; Ensure; Geometry; Glass; Goat; Hybrids; Image; Immunoglobulin G; Label; Light; Low income; Maps; Measures; Methods; Molecular; Molecular Probes; Morphology; Mus; Nanostructures; Optics; Oregon; Physicians; Process; Protocols documentation; Refractive Indices; Research; Resolution; Shapes; Signal Transduction; Silicon Dioxide; Silver; Specificity; Spectrum Analysis; Structure; Surface; Techniques; Universities; base; chemical reaction; clinically relevant; cost; cost effective; electric field; improved; in vivo; meetings; nano; nanofabrication; nanomaterials; nanoparticle; nanoscale; optical imaging; optical sensor; photonics; plasmonics; point of care; public health relevance; screening; sensor; two-dimensional

 DESCRIPTION (provided by applicant): Plasmonic biosensors have greatly overcome the limitations of conventional optical sensors in terms of sensitivity, tunability, photo-stability, ad in vivo applicability. However, the concerns with average sensitivity, detection specificity, surface functionalities, and device expense still cannot meet the application requirement of point-of-care and personal diagnosis. In this research, the PIs at Oregon State University propose to explore dual-mode plasmonic biosensors using bioenabled nanomaterials --- diatom biosilica, with active surface-functionalities as affordable and eco-friendly integration platforms of Ag nanoparticles for label-free detection of biomolecules. Diatoms are single-celled algae that make silica shells or frustules with intricate nanoscale features imbedded within periodic two-dimensional pore arrays. The essence of this research is addressed by exploration of the unique Fano-resonant hybrid modes between silver nanoparticles and diatom frustules, which leads to high-Q resonant peaks and enhanced local electric field that can significantly enhance the light-matter interactions. Dual-mode plasmon sensing mechanisms, including surface-enhanced Raman scattering (SERS) and refractive-index (RI) sensing will be simultaneously implemented on the plasmonic-biosilica nanostructures to obtain quantitative biosensing with structural resolution of the biomolecules. In addition, the nano-corrugated surface of diatom frustules will help to increase the possibility of capturing various biomolecules. Other exclusive advantages include affordable cost and eco- friendly fabrication of the sensor chips that are completely free of expensive photolithography and other nanofabrication processes, and easy expandability to sensor arrays for high throughput diagnostics, which can provide greater accessibility for large-scale screening. Such unique plasmonic-biosilica sensors with unprecedented figure-of-merits can be used as disposable biosensors to acquire clinically relevant information for the physician and clinician in point-of-care, personal diagnosis, as well as for disease detection in low- income developing countries.

 描述(申请人提供)：等离子体生物传感器在灵敏度、可调性、光稳定性、体内适用性等方面极大地克服了传统光学传感器的局限性。然而，对平均灵敏度、检测特异度、表面功能和设备费用的关注仍然不能满足医疗保健和个人诊断的应用需求。在这项研究中，俄勒冈州立大学的PI建议探索使用生物使能纳米材料-硅藻生物二氧化硅的双模等离子体生物传感器，具有活跃的表面功能，作为负担得起的、环保的银纳米颗粒集成平台，用于生物分子的无标记检测。硅藻是单细胞藻类，它们制造具有复杂纳米级特征的硅壳或锥体，嵌入周期性的二维孔隙阵列中。本研究的实质是通过探索银纳米粒子与硅藻晶体之间独特的Fano共振混合模式，导致高Q共振峰和增强的局域电场，从而显著增强光-物质相互作用。双模等离子体传感机制，包括表面增强拉曼散射(SERS)和折射率(RI)传感，将同时在等离子体-生物二氧化硅纳米结构上实现，以获得具有结构分辨率的生物分子的定量生物传感。此外，硅藻的纳米波纹表面将有助于增加捕获各种生物分子的可能性。其他独家优势包括完全不需要昂贵的光刻和其他纳米制造工艺的传感器芯片的可承受成本和环保制造，以及易于扩展到传感器阵列以实现高通量诊断，这可以为大规模筛查提供更大的可及性。这种独特的等离子体-生物硅传感器具有前所未有的优势系数，可用作一次性生物传感器，为医生和临床医生在医疗保健、个人诊断以及低收入发展中国家的疾病检测中获取临床相关信息。