Dual-Mode Plasmonic Biosensors using Bioenabled Nanomaterials

 描述（由申请人提供）：等离子体生物传感器在灵敏度、可调谐性、光稳定性和体内适用性方面大大克服了传统光学传感器的局限性。然而，对平均灵敏度、检测特异性、表面功能和设备费用的关注仍然不能满足即时护理和个人诊断的应用要求。在这项研究中，在俄勒冈州州立大学的PI建议探索双模式等离子体生物传感器使用bioenabled纳米材料--硅藻生物硅，具有活性表面功能作为负担得起的和生态友好的集成平台的银纳米粒子的生物分子的无标记检测。硅藻是单细胞藻类，它们制造二氧化硅外壳或硅藻壳，这些外壳或硅藻壳具有嵌入周期性二维孔阵列中的复杂纳米级特征。本研究的本质是通过探索银纳米颗粒和硅藻硅藻细胞壳之间独特的Fano共振混合模式来解决的，该模式导致高Q共振峰和增强的局部电场，可以显着增强光-物质相互作用。双模式等离子体传感机制，包括表面增强拉曼散射（Sers）和折射率（RI）传感将同时实施的等离子体-生物二氧化硅纳米结构，以获得定量生物传感与生物分子的结构分辨率。此外，硅藻硅藻壳的纳米波纹表面将有助于增加捕获各种生物分子的可能性。其他独特的优点包括传感器芯片的可负担的成本和生态友好的制造，其完全没有昂贵的光刻和其他纳米纤维工艺，以及易于扩展到用于高通量诊断的传感器阵列，这可以为大规模筛选提供更大的可访问性。具有前所未有的品质因数的这种独特的等离子体生物二氧化硅传感器可以用作一次性生物传感器，以在护理点、个人诊断以及低收入发展中国家的疾病检测中为医生和临床医生获取临床相关信息。

 DESCRIPTION (provided by applicant): Plasmonic biosensors have greatly overcome the limitations of conventional optical sensors in terms of sensitivity, tunability, photo-stability, ad in vivo applicability. However, the concerns with average sensitivity, detection specificity, surface functionalities, and device expense still cannot meet the application requirement of point-of-care and personal diagnosis. In this research, the PIs at Oregon State University propose to explore dual-mode plasmonic biosensors using bioenabled nanomaterials --- diatom biosilica, with active surface-functionalities as affordable and eco-friendly integration platforms of Ag nanoparticles for label-free detection of biomolecules. Diatoms are single-celled algae that make silica shells or frustules with intricate nanoscale features imbedded within periodic two-dimensional pore arrays. The essence of this research is addressed by exploration of the unique Fano-resonant hybrid modes between silver nanoparticles and diatom frustules, which leads to high-Q resonant peaks and enhanced local electric field that can significantly enhance the light-matter interactions. Dual-mode plasmon sensing mechanisms, including surface-enhanced Raman scattering (SERS) and refractive-index (RI) sensing will be simultaneously implemented on the plasmonic-biosilica nanostructures to obtain quantitative biosensing with structural resolution of the biomolecules. In addition, the nano-corrugated surface of diatom frustules will help to increase the possibility of capturing various biomolecules. Other exclusive advantages include affordable cost and eco- friendly fabrication of the sensor chips that are completely free of expensive photolithography and other nanofabrication processes, and easy expandability to sensor arrays for high throughput diagnostics, which can provide greater accessibility for large-scale screening. Such unique plasmonic-biosilica sensors with unprecedented figure-of-merits can be used as disposable biosensors to acquire clinically relevant information for the physician and clinician in point-of-care, personal diagnosis, as well as for disease detection in low- income developing countries.