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Endodermal regulation of primordial germ cells

原始生殖细胞的内胚层调节

基本信息

批准号：
8951810
负责人：
Jeremy Nance
金额：
$ 21.19万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-07-13 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8951810
关键词：
Actins Adult Affect Apoptotic Behavior Biological Bite Caenorhabditis elegans Cannibalism Cell Communication Cell physiology Cell surface Cells Cytoplasm Defect Digestion Eating Embryo Embryonic Development Endoderm Endoderm Cell Event Excision Exhibits Fertility Future Gastrointestinal tract structure Genes Genetic Genetic Screening Genome Germ Cells Germ Lines Goals Gonadal structure Hour Image Invertebrates Lead Left Life Lobe Mediating Mitochondria Modeling Mutation Nematoda Neurons Oranges Organelles Phosphatidylserines Photoreceptors Process Regulation Role Signal Transduction Site Somatic Cell Staging Sterility Structure of primordial sex cell Surface Teratoma Testing Vertebrates base cell behavior egg insight migration mutant novel polymerization prevent programs protein expression public health relevance research study response sperm cell tool tumor

项目摘要

DESCRIPTION (provided by applicant): Primordial germ cells (PGCs) are embryonic precursors of the adult germ line. At early stages of embryogenesis, PGCs segregate from somatic cells and exhibit distinctive behaviors and regulation. One conserved PGC behavior, which occurs in both invertebrates and vertebrates, is physical association with endodermal cells. The significance of PGC-endoderm interactions is not known. By performing live fluorescent imaging of PGCs and endoderm in the anatomically simple C. elegans embryo, we have discovered an unexpected and novel role for endoderm in remodeling PGC contents. Specifically, we observe that PGCs extend large cytoplasmic lobes, which are actively bitten off and digested by endodermal cells. Endoderm-assisted remodeling allows PGCs to discard cellular components, such as the majority of their mitochondria, and we hypothesize that remodeling prepares PGCs for their impending transition to adult germ cells. The broad goal of this exploratory proposal is to establish the C. elegans embryo as a model to investigate the purpose and mechanism of endoderm-PGC interactions. Our specific goals are to (1) to test the hypothesis that a subset of cell corpse engulfment genes promotes endoderm-assisted PGC remodeling; and (2) to clone and characterize existing mutants, and to identify new mutants, which fail in PGC remodeling. These studies will provide a basis for establishing whether endoderm-assisted PGC remodeling is important for adult germ cell function in C. elegans and other species. In addition, our findings may yield new insights into conceptually analogous remodeling events, such as the shedding of photoreceptor outer segments and the remodeling of neurons.