Mouse Metabolic Phenotyping Core (Core C)

小鼠代谢表型核心（核心 C）

• 批准号: 8933739
• 负责人: Ruth Gordillo
• 金额: $ 24.23万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8933739
• 关键词: Adipocytes; Animal Model; Back; Biological Assay; Body Composition; Brain; Calorimetry; Cell Separation; Chemistry; Cholesterol; Collection; Communication; Data; Disease; Dual-Energy X-Ray Absorptiometry; Euglycemic Clamping; Food; Free Will; Funding; Glucose; Glucose Clamp; Guidelines; Hepatocyte; Individual; Laboratories; Lipids; Magnetic Resonance Imaging; Maps; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Mus; Obesity; Pathogenesis; Phenotype; Physiological; Plasma; Quality Control; Rattus; Reading; Reagent; Research; Research Personnel; Research Project Grants; Services; Speed; Sphingolipids; Technical Expertise; Technology; Tissue Sample; Tissues; Triglycerides; Uridine; Urine; Water consumption; Work; abstracting; cost effective; glucose and insulin clamps; instrumentation; liquid chromatography mass spectrometry; magnetic beads; metabolomics; microCT; novel; prevent; sound

Abstract The services in the Mouse Metabolic Phenotyping Core (Core C) draw upon the expertise of many investigators at UTSW to provide comprehensive analytical capabilities that are on par with the best facilities in the world. We are looking back on 5 successful years during which we have expanded our assay capabilities and supported Projects 1 through 3 during the ongoing funding period with our comprehensive phenotyping panel under the direction of Dr. Jay Horton. We have made major steps towards establishing a state-of-the-art sphingolipidomics suite beyond the originally described standard phenotyping panel. The availability of standardized assays speeds the pace of our research, facilitates inter-lab comparisons of data, and provides a common platform for the necessary close interactions between investigators. Animal models that allow careful characterization of the metabolic effects of obesity and that have an altered communication axis between the three critical tissues studied here are vital to understand the pathogenesis of disease. Given the number of phenotypic measurements, the range of target metabolites, and the number of different animal models investigated, it is necessary to provide uniform measurements and to have quality control guidelines in place. To facilitate the research for Projects 1-3, Core C will provide comprehensive, state-of-the-art measurements of multiple parameters, with measurements involving mass spectrometry and physiological read outs that include lipid, glucose, and metabokine assays. The technical expertise Core C offers will bring multiple benefits to project investigators, including the following:  Centralized Core services are more cost effective and prevent the duplication of reagents across PPG investigators.  Provides consistency in various assays across laboratories.  Provides consistent quality control measures.  Makes available technically challenging assays, such as hyperinsulinemic-euglycemic clamps, that cannot be established in individual laboratories.  Frees investigators from routine work to focus on the intellectual challenges of the projects.

摘要 老鼠代谢表型核心(核心C)的服务利用了许多人的专业知识 UTSW的调查人员提供全面的分析能力，与 整个世界。我们回顾了成功的五年，在这五年中，我们扩大了检测能力 并在正在进行的资助期内通过我们的全面表型分析支持项目1至3 小组在杰伊·霍顿博士的指导下进行。我们已经朝着建立一个最先进的 鞘脂组学套件超越了最初描述的标准表型小组。 标准化分析的可用性加快了我们的研究步伐，促进了实验室之间的比较 数据，并为调查人员之间必要的密切互动提供了一个共同平台。 动物模型，允许仔细描述肥胖的代谢影响，并改变了 在此研究的三个关键组织之间的通信轴对于了解糖尿病的发病机制至关重要。 疾病。给定表型测量的数量，目标代谢物的范围，以及 调查不同的动物模型，要提供统一的测量结果，并要有质量 制定了控制指南。为了促进项目1-3的研究，核心C将提供全面的、 最先进的多参数测量，包括质谱学和 生理学读数，包括脂肪、葡萄糖和代谢物分析。 Core C提供的技术专长将为项目调查人员带来多方面的好处，包括： 集中核心服务更具成本效益，并防止跨PPG的试剂重复 调查人员。 在不同实验室的各种检测中提供了一致性。 提供始终如一的质量控制措施。 提供了具有技术挑战性的分析方法，如高胰岛素-正常血糖钳夹， 不能在个别实验室建立。 将调查人员从例行公事中解放出来，专注于这些项目的智力挑战。