Mouse Metabolic Phenotyping Core (Core C)

小鼠代谢表型核心（核心 C）

• 批准号: 9306028
• 负责人: Ruth Gordillo
• 金额: $ 23.55万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9306028
• 关键词: Adipocytes; Animal Model; Back; Biological Assay; Body Composition; Brain; Calorimetry; Cell Separation; Chemistry; Cholesterol; Collection; Communication; Data; Data Analytics; Disease; Dual-Energy X-Ray Absorptiometry; Euglycemic Clamping; Food; Free Will; Funding; Glucose; Guidelines; Hepatocyte; Individual; Laboratories; Lipids; Magnetic Resonance Imaging; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Mus; Obesity; Pathogenesis; Phenotype; Physiological; Plasma; Quality Control; Rattus; Reagent; Research; Research Personnel; Research Project Grants; Services; Speed; Sphingolipids; Standardization; Technical Expertise; Technology; Tissue Sample; Tissues; Triglycerides; Uridine; Urine; Water consumption; Work; cost effective; glucose and insulin clamps; instrumentation; liquid chromatography mass spectrometry; magnetic beads; metabolic phenotype; metabolomics; microCT; novel; prevent; sound; treadmill

Abstract The services in the Mouse Metabolic Phenotyping Core (Core C) draw upon the expertise of many investigators at UTSW to provide comprehensive analytical capabilities that are on par with the best facilities in the world. We are looking back on 5 successful years during which we have expanded our assay capabilities and supported Projects 1 through 3 during the ongoing funding period with our comprehensive phenotyping panel under the direction of Dr. Jay Horton. We have made major steps towards establishing a state-of-the-art sphingolipidomics suite beyond the originally described standard phenotyping panel. The availability of standardized assays speeds the pace of our research, facilitates inter-lab comparisons of data, and provides a common platform for the necessary close interactions between investigators. Animal models that allow careful characterization of the metabolic effects of obesity and that have an altered communication axis between the three critical tissues studied here are vital to understand the pathogenesis of disease. Given the number of phenotypic measurements, the range of target metabolites, and the number of different animal models investigated, it is necessary to provide uniform measurements and to have quality control guidelines in place. To facilitate the research for Projects 1-3, Core C will provide comprehensive, state-of-the-art measurements of multiple parameters, with measurements involving mass spectrometry and physiological read outs that include lipid, glucose, and metabokine assays. The technical expertise Core C offers will bring multiple benefits to project investigators, including the following:  Centralized Core services are more cost effective and prevent the duplication of reagents across PPG investigators.  Provides consistency in various assays across laboratories.  Provides consistent quality control measures.  Makes available technically challenging assays, such as hyperinsulinemic-euglycemic clamps, that cannot be established in individual laboratories.  Frees investigators from routine work to focus on the intellectual challenges of the projects.

摘要 小鼠代谢表型核心（核心C）中的服务借鉴了许多 研究人员在UTSW提供全面的分析能力，与最好的设施， 世界我们正在回顾成功的5年，在此期间，我们扩大了我们的分析能力 在持续的资助期间，我们通过全面的表型分析支持了项目1至3 在杰伊·霍顿博士的指导下我们在建立一个最先进的 鞘脂组学套件超出了最初描述的标准表型组。 标准化检测的可用性加快了我们研究的步伐，促进了实验室间的比较， 数据，并为调查人员之间进行必要的密切互动提供了一个共同平台。 动物模型，允许仔细表征肥胖的代谢影响，并具有改变的 在这里研究的三个关键组织之间的通讯轴对于理解 疾病考虑到表型测量的数量、靶代谢物的范围和代谢物的数量， 研究不同的动物模型，有必要提供统一的测量结果， 控制准则到位。为方便项目1-3的研究，核心C将提供全面的， 多个参数的最新测量，包括质谱测量， 生理读数，包括脂质、葡萄糖和代谢因子测定。 核心C提供的技术专长将为项目调查人员带来多重好处，包括： ·集中的核心服务更具成本效益，并防止PPG之间的试剂重复 investigators. 在不同实验室的各种检测中提供一致性。 提供一致的质量控制措施。 提供技术上具有挑战性的分析，如高胰岛素-正常血糖钳夹， 不能在个别实验室建立。 将调查人员从日常工作中解放出来，专注于项目的智力挑战。