A novel cytosolic chaperone complex in polyomavirus ER membrane transport

 描述(申请人提供)：本申请的目的是阐明一种新的胞质伴侣复合体如何促进无包膜病毒多瘤病毒(Py)的内质网(ER)膜穿透。为了引起感染，Py与细胞膜上的宿主糖脂受体结合，被内吞，并运输到内质网管腔。在这里，病毒颗粒经历构象变化，穿透ER膜，到达细胞质。病毒从胞浆运输到细胞核，在那里病毒基因组的转录和复制随后发生，导致裂解感染或细胞转化。Py如何从内质网进入细胞质是一个关键但知之甚少的事件。特别令人费解的是调节这种内质网膜穿透过程的细胞质事件。因此，这项应用集中在阐明所谓的BHS胞浆伴侣复合体在将Py从内质网射入胞浆中的作用。

 DESCRIPTION (provided by applicant): The objective of this application is to clarify how a novel cytosolic chaperone complex promotes endoplasmic reticulum (ER) membrane penetration of the non-enveloped virus polyomavirus (Py). To cause infection, Py engages a host glycolipid receptor on the plasma membrane, becomes endocytosed, and traffics to the ER lumen. Here the viral particle undergoes conformational changes to penetrate the ER membrane and reach the cytosol. From the cytosol, the virus transports to the nucleus where transcription and replication of the viral genome ensue, leading to lytic infection or cellular transformation. How Py enters the cytosol from the ER is a crucial yet poorly understood event. Particularly enigmatic are cytosolic events that regulate this ER membrane penetration process. Accordingly this application is focused on elucidating the role of the so-called BHS cytosolic chaperone complex in ejecting Py into the cytosol from the ER.