How polyomaviruses penetrate the ER membrane

多瘤病毒如何穿透内质网膜

• 批准号: 8374100
• 负责人: Billy Tsai
• 金额: $ 32.35万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2016-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8374100
• 关键词: Binding; Biological Assay; Cell Nucleus; Cell surface; Cells; Cytosol; Data; ERp57; Endoplasmic Reticulum; Event; Family; Family member; Foundations; Gangliosides; Genetic Transcription; Individual; Infection; Integration Host Factors; Laboratories; Lipid Bilayers; Lytic Phase; Membrane; Molecular; Pathway interactions; Penetration; Polyomavirus; Process; Protein Disulfide Isomerase; Proteins; Reaction; Simian virus 40; Text; Transmembrane Transport; Viral; Virus; base; cell transformation; disulfide bond; endoplasmic reticulum glycoprotein p72; experience; glycolipid receptor; insight; mouse polyomavirus; multicatalytic endopeptidase complex; novel; particle; tumor; viral DNA

DESCRIPTION (provided by applicant): This application aims to elucidate the molecular mechanism by which the two non-enveloped viruses polyomavirus (Py) and SV40 penetrate the endoplasmic reticulum (ER) membrane. To infect cells, Py and SV40 bind to glycolipid receptors called gangliosides on the host cell surface and are internalized. The viruses are then transported to the lumen of the ER where they co-opt host cell machineries to cross the ER membrane and reach the cytosol. From the cytosol, Py and SV40 are transported into the nucleus where transcription and replication of the viral DNA ensue, leading to lytic infection or cell transformation. How these viruses penetrate the ER membrane to reach the cytosol, a decisive infection event, remains mysterious and is a process we intend to clarify in this proposal.

描述（由申请方提供）：本申请旨在阐明两种无包膜病毒多瘤病毒（Py）和SV40穿透内质网（ER）膜的分子机制。为了感染细胞，Py和SV40与宿主细胞表面称为神经节苷脂的糖脂受体结合并内化。然后病毒被运输到ER的内腔，在那里它们协同宿主细胞机器穿过ER膜并到达胞质溶胶。Py和SV40从胞质溶胶被转运到细胞核中，在那里病毒DNA的转录和复制随之发生，导致裂解感染或细胞转化。这些病毒如何穿透内质网膜到达胞质溶胶，这是一个决定性的感染事件，仍然是一个谜，这是我们打算在本提案中澄清的过程。