Electron Microscopy Core

电子显微镜核心

• 批准号: 9157591
• 负责人: NICO TJANDRA
• 金额: $ 2.26万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9157591
• 关键词: Antigens; Carbon; Cell surface; Cells; Contractor; Core Facility; Cultured Cells; Cytoskeleton; Electron Microscope; Electron Microscopy; Image; Immunogold Technics; Light Microscope; Membrane; Membrane Lipids; Microtomy; Molecular Structure; National Heart, Lung, and Blood Institute; Negative Staining; Organ; Organism; Platinum; Polymers; Postdoctoral Fellow; Principal Investigator; Proteins; Publishing; Research; Resolution; Scanning; Scanning Electron Microscopy; Scientist; Services; Shadowing (Histology); Structure; Students; Techniques; Tissues; Training; Transmission Electron Microscopy; Vesicle; Work; digital imaging; immunocytochemistry; journal article; sample fixation; transmission process

The NHLBI Electron Microscopy Core Facility has supported projects using the following techniques in the past year: 1. Fixation, embedding, ultra-thin sectioning and transmission EM digital imaging of tissues and cells. 2. EM immunocytochemistry, including immunogold localization of proteins and other antigens within and on the surface of cells by pre-embedding and cryo-immunogold techniques. 3. Negative staining of large proteins, polymers and supramolecular structures as well as lipid and membrane vesicles for transmission EM imaging as well as platinum shadowing of large molecules. 4. Fixation, critical point drying, sputter-coating and scanning EM digital imaging of small organisms, organs, tissues and cells, as well as other materials such as artificial matrices. 5. Platinum carbon replicas of extracted, critical point dried cytoskeletons and the substrate-attached membranes of cultured cells In the past year, we have supported 45 projects for 27 principal investigators in the NHLBI/DIR and 6 project for a scientist outside the DIR. During this period, 14 journal articles have been published presenting from work in the EM Core with 12 where the EM Core Staff were co-authors or the EM Core was acknowledged. We have trained 14 postdoctoral fellows, students and contractors in the use of the scanning and transmission electron microscopes.

在过去的一年中，NHLBI电子显微镜核心设施支持了使用以下技术的项目： 1.组织和细胞的固定、包埋、超薄切片和透射电镜数字成像。 2. EM免疫细胞化学，包括通过预包埋和冷冻免疫金技术对细胞内和表面的蛋白质和其他抗原进行免疫金定位。 3.大蛋白质、聚合物和超分子结构以及脂质和膜囊泡的负染色，用于透射EM成像以及大分子的铂阴影。 4.固定，临界点干燥，涂层和扫描EM小生物体，器官，组织和细胞的数字成像，以及其他材料，如人工基质。 5. 培养细胞的提取、临界点干燥细胞骨架和基质附着膜的铂碳复制品 在过去的一年里，我们支持了NHLBI/NHLBI的27名主要研究人员的45个项目，以及NHLBI/NHLBI以外的一名科学家的6个项目。在此期间，发表了14篇期刊文章，介绍了EM核心的工作，其中12篇是EM核心工作人员的共同作者或EM核心得到了认可。 我们已经培训了14名博士后研究员，学生和承包商在使用扫描和透射电子显微镜。