Effects of Tart Cherry and Aroniaberry Supplementation on Endothelial Function and Cardiovascular Measures in Healthy Older Adults: Interactions with Genotype and Proteome

酸樱桃和野樱莓补充剂对健康老年人内皮功能和心血管指标的影响：与基因型和蛋白质组的相互作用

• 批准号: 9148049
• 负责人: Mary Engler
• 金额: $ 20.29万
• 依托单位: NATIONAL INSTITUTE OF NURSING RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9148049
• 关键词: Age; Aging; Aging-Related Process; Ankle; Antioxidants; Atherosclerosis; Berry; Biological Markers; Blood Vessels; Cardiovascular Diseases; Cardiovascular Physiology; Cause of Death; Cherry - dietary; Clinical Trials; Consumption; Data; Diet; Dietary Intervention; Double-Blind Method; Elderly; Failure; Flavonoids; Fruit; Future; Genetic; Genomics; Genotype; Health; Heart; Human; Individual; Inflammation; Injury; Intervention; Investigation; Link; Lipids; Measurement; Measures; Mediating; Nitric Oxide; Nutrient; Outcome Study; Oxidative Stress; Pathway interactions; Physiological; Physiological Processes; Placebo Control; Placebos; Plasma; Plasma Proteins; Property; Proteome; Proteomics; Randomized; Risk; Role; Single Nucleotide Polymorphism; Structure; Supplementation; Surrogate Markers; Therapeutic; Visit; age related; arterial stiffness; brachial artery; cardiovascular disorder risk; cardiovascular risk factor; disability; disorder risk; endothelial dysfunction; fruits and vegetables; immune function; improved; indexing; nutritional genomics; oxidation; phenolic acid; prevent; response; vascular inflammation

Cardiovascular disease (CVD) continues to be the leading cause of death and disability worldwide. Cardiovascular disease can be age-related in both onset and progression. The aging process causes failure in protective vascular mechanisms leading to pathophysiological changes and increased risk of CVD. Heart healthy strategies including consumption of diets rich in fruits and vegetables reduce risk and prevent CVD. There is growing experimental evidence that certain fruits and berries including, cherries and aroniaberries, may be cardioprotective due to their antioxidant and vasodilatory properties, and beneficial modulation of immune function and inflammation. However, there are limited human studies that have examined these effects. In older adults, vascular aging predominantly includes increased arterial stiffness and endothelial dysfunction. Oxidative stress and inflammation are also linked to the aging process. Therefore, this study will investigate the effects of tart cherry and aroniaberry supplementation on endothelial function (flow-mediated brachial artery dilation) and arterial stiffness (cardio-ankle vascular index) as indicators of cardiovascular risk and atherosclerosis. These measurements also provide an assessment of arterial structure and function. Biomarkers of endothelial dysfunction, inflammation, vascular injury, oxidation, lipids, nitric oxide status, as well as flavonoid and phenolic acid profiles will also be examined. Importantly, the role of genetics and its environmental interactions as causal factors in CVD is not completely understood. There is also limited data on the plasma proteome and the changes in physiological processes that may result from interventions with diet or nutrients. This study will explore the association between responses to tart cherry or aroniaberry supplementation and single nucleotide polymorphisms (SNPs) in loci related to the study outcomes and to the risk of CVD. Moreover, the association between these two supplements and a proteomic panel of plasma proteins involved in several physiological pathways and CVD will also be investigated. In a randomized, double-blind, placebo-controlled, crossover clinical trial, 96 healthy older adults (ages 55-70) will be given tart cherry alternating with aroniaberry supplementation daily for 3 weeks each while on their usual diet. This study will include 6 visits at 3 week intervals consisting of treatment, washout and placebo periods. This investigation will provide important information on the role of flavonoid-rich nutrients and their effect on arterial structure and function. Relating genomic and proteomic signatures to the outcomes of this study will be useful in developing future therapeutic strategies to lower CVD risk.

心血管疾病(CVD)仍然是世界范围内死亡和残疾的主要原因。心血管疾病的发病和进展都可能与年龄有关。衰老过程导致保护性血管机制失效，导致病理生理变化和心血管疾病风险增加。心脏健康策略，包括食用富含水果和蔬菜的饮食，降低风险和预防心血管疾病。越来越多的实验证据表明，某些水果和浆果，包括樱桃和龙舌兰，可能具有心脏保护作用，因为它们具有抗氧化和血管扩张特性，并有助于调节免疫功能和炎症。然而，检验这些影响的人体研究有限。在老年人中，血管老化主要包括动脉僵硬增加和内皮功能障碍。氧化应激和炎症也与衰老过程有关。因此，本研究将研究补充酸樱桃和龙舌兰对血管内皮功能(血流介导的臂动脉扩张)和动脉僵硬(心踝血管指数)的影响，以此作为心血管风险和动脉粥样硬化的指标。这些测量还提供了动脉结构和功能的评估。还将检查内皮功能障碍、炎症、血管损伤、氧化、血脂、一氧化氮状态以及类黄酮酸和酚酸的生物标志物。重要的是，遗传学及其环境相互作用作为心血管疾病的致病因素的作用还没有完全了解。关于血浆蛋白质组和饮食或营养干预可能导致的生理过程变化的数据也有限。这项研究将探索对补充酸樱桃或龙舌兰的反应与与研究结果和心血管疾病风险相关的基因座的单核苷酸多态(SNPs)之间的关联。此外，还将研究这两种补充剂与涉及几种生理途径和心血管疾病的血浆蛋白质组之间的联系。在一项随机、双盲、安慰剂对照的交叉临床试验中，96名健康的老年人(年龄55-70岁)在正常饮食的同时，每天交替服用酸樱桃和龙舌兰补充剂，为期3周。这项研究将包括6次访问，间隔3周，包括治疗、洗涤和安慰剂阶段。这项研究将为研究富含黄酮类的营养素的作用及其对动脉结构和功能的影响提供重要信息。将基因组和蛋白质组特征与这项研究的结果联系起来，将有助于开发未来的治疗策略，以降低心血管疾病的风险。