Administrative Core

行政核心

• 批准号: 8651877
• 负责人: Mark Larche
• 金额: $ 11.13万
• 依托单位: MCMASTER UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8651877
• 关键词: Address; Allergens; Asthma; Blood; Blood specimen; Bone Marrow; Bronchoalveolar Lavage; Cells; Clinical; Disease; Epitopes; Felis catus; Frequencies; Goals; Hypersensitivity; Immunotherapy; Intervention; Lung; MHC Class II Genes; Memory; Pathogenesis; Peptides; Phase II Clinical Trials; Phenotype; Production; Public Health; Reagent; Regulatory T-Lymphocyte; Research; Research Infrastructure; Research Project Grants; Sampling; Site; T-Lymphocyte; United States National Institutes of Health; Validation; chemokine receptor; cytokine; improved; in vivo Model; novel; novel strategies; pre-clinical; programs; prospective; response

The overall long-term objective of the Administrative Core will be to provide scientific direction & oversight, project management and fiscal & administrative infrastructure to support all of the Projects and Cores within the Allergen Epitope Research and Validation Center. The goal of the Administrative Core is to optimally facilitate the scientific objectives and goals of the Center. The scientific objectives and goals of the Center are to use in vivo models (clinical and pre-clinical) to identify, enrich and characterize allergen-specific T cells in order to understand how their frequency and functional phenotype changes in response to an allergen-specific intervention. In Project 1, blood samples from two Phase II clinical trial of peptide immunotherapy (one historic and one prospective) will be subjected to allergen-specific enrichment of T cells using novel MHC Class II tetramer reagents. Tetramer+ T cells will be characterized for frequency, chemokine receptor expressoin, memory phenotype, cytokine production profiles and regulatory T cell frequency. In Project 2, blood, bone marrow and bronchoalveolar lavage samples will be obtained before and after segmental lung allergen challenge with cat allergen extract. Fel d 1 tetramers will be emioyed to enrich allergen-specific T cells from each compartment and these cells will be characterized as above.The Administrative Core will support these objectives through the following Specific Aims; Aim 1: to provide scientific direction, guidance and strategy for the Center through interaction between the Center Management Committee (CMC), the NIH Program Officers and the Scientific Advisory Panel (SAP), Aim 2: to provide (a) project management, (b) coordination of activities/information between projects and sites (3) general administrative support to the Center, Aim 3: to provide fiscal management within the Center (between the three sites) and between the Center and the NIH.

行政核心的总体长期目标将是提供科学指导和监督、项目管理以及财政和行政基础设施，以支持阿尔尔根表观研究和验证中心内的所有项目和核心。行政核心的目标是以最佳方式促进中心的科学目标和目标。该中心的科学目标和目标是使用活体模型(临床和临床前)来识别、丰富和表征过敏原特异性T细胞，以便了解它们的频率和功能表型在过敏原特异性干预后如何变化。在项目1中，来自多肽免疫治疗的两个第二阶段临床试验(一个是历史的，一个是未来的)的血液样本将接受过敏原特异性T细胞的浓缩 使用新型MHC II类四聚体试剂。将对四聚体+T细胞的频率、趋化因子受体表达、记忆表型、细胞因子产生谱和调节性T细胞频率进行表征。在项目2中，将在用猫变应原提取物节段性肺变应原攻击前后采集血液、骨髓和支气管肺泡灌洗样本。FEL D 1四聚体将被激活 从每个隔室中丰富过敏原特异性T细胞，这些细胞的特征将如上所述。行政核心将通过以下具体目标支持这些目标；目标1：通过中心管理委员会(CMC)、NIH项目官员和科学咨询小组(SAP)之间的互动，为中心提供科学指导、指导和战略，目标2： 提供(A)项目管理，(B)协调项目和地点之间的活动/信息(3)向中心提供一般行政支助，目标3：在中心内(三个地点之间)以及中心和国家卫生研究院之间提供财政管理。