Mechanisms of Radical SAM Enzymes Probed by EPR Spectroscopy
EPR 光谱探讨自由基 SAM 酶的作用机制
基本信息
- 批准号:8632910
- 负责人:
- 金额:$ 31.59万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-09-15 至 2018-08-31
- 项目状态:已结题
- 来源:
- 关键词:9-mercaptodethiobiotinActive SitesAffectAnabolismAntibioticsAnticodonArizonaBerlinBindingBiochemicalBiologicalBiologyBiotinChemicalsChemistryCleaved cellCodon NucleotidesComplexDNA Sequence RearrangementData SetDivalent CationsElectron Nuclear Double ResonanceElectron Spin Resonance SpectroscopyElectronicsElectronsEnvironmentEnzymesEquipmentEventExcisionFamilyFelis catusFundingGoalsGuanineHawaiiHealthHumanHydrogenHydrogenaseIonsIsotope LabelingKineticsLabelLaboratoriesLearningLigandsLightLyaseMagnetic ResonanceMagnetismMass Spectrum AnalysisMeasuresMetalsMethodsModelingModificationNatureNitrogenNucleoside QOxygenPathway interactionsPlayPositioning AttributePreparationPropertyProtein BiosynthesisProteinsProtonsRNARadioisotopesReactionRestS-AdenosylmethionineSamplingSequence AnalysisSideSiteSpectroscopy, Fourier Transform InfraredSpectrum AnalysisStructureSulfurTimeTransfer RNATyrosineUnited States National Institutes of HealthUniversitiesVitaminsWorkbasebiotin synthasechemical reactiondesigndesthiobiotindivalent metalelectronic structureenzyme activitygeometric structureimprovedinstrumentinterestiron hydrogenasepublic health relevanceresearch studytetrahydropterinthiophanevitamin biosynthesis
项目摘要
Project Summary
Nature uses highly reactive radicals to carry out a diverse set of biochemical functions, many of which are essen-
tial to maintaining proper human health. These potent biological radical reactions need to be carried out safely,
producing essential specific products, without dangerous side reactions occurring. A large number of such rad-
ical reactions are performed by the family of radical SAM enzymes, which use a [4Fe-4S] center with a bound
S-adenosylmethionine (SAM) molecule to generate a strongly oxidizing 5'-deoxyadenosyl radical which can in
turn drive a large number of difficult chemical reactions. We will target mechanistic aspects of several classes of
radical SAM enzymes. Biotin synthase is a radical SAM enzyme that catalyzes the final step in the biosynthesis
of the vitamin biotin. A set of Fe-S and radical SAM maturase enzymes are used to build the unique Fe-S center
of Fe-Fe hydrogenase, an enzyme which catalyzes the important reduction of protons to dihydrogen and vice
versa. And radical SAM enzymes are used to modify many bases in transfer RNA, improving codon-anticodon
recognition in order to make protein synthesis more reliable. We are specifically interested in a radical SAM
enzyme QueE that is essential for generating 7-deazapurines. This proposal describes a magnetic resonance
spectroscopic approach to study such diverse radical SAM enzymes. Specifically, we are using electron para-
magnetic resonance (EPR) spectroscopy, which can precisely measure the magnetic environment of unpaired
electrons in the radical SAM Fe-S clusters, in the organic radicals that these clusters generate, and in secondary
metal centers that are involved in the reactions in many of these enzymes.
项目摘要
自然界使用高活性自由基来执行各种生化功能,其中许多是埃森的,
有助于维持人体健康这些强有力的生物自由基反应需要安全地进行,
生产必需的特定产品,而不会发生危险的副反应。一大批这样的人--
通过自由基SAM酶家族进行化学反应,其使用具有结合的[4Fe-4S]中心。
S-腺苷甲硫氨酸(SAM)分子产生强氧化性5 ′-脱氧腺苷自由基,
转驱动大量困难的化学反应。我们将针对几类机械方面的
自由基SAM酶。生物素合成酶是一种自由基SAM酶,催化生物合成的最后一步
维生素生物素。一组Fe-S和自由基SAM成熟酶用于构建独特的Fe-S中心
铁-铁氢化酶是一种催化质子还原为二氢和二氢的重要酶,
亦然自由基SAM酶被用来修饰转移RNA中的许多碱基,提高密码子-反密码子的效率,
为了使蛋白质合成更可靠。我们对一种激进的SAM特别感兴趣
QueE酶是产生7-脱氮嘌呤所必需的。这个提议描述了一种磁共振
光谱方法来研究这种不同的自由基SAM酶。具体来说,我们使用电子帕拉-
磁共振(EPR)光谱,它可以精确地测量未配对的磁环境,
自由基SAM Fe-S簇中的电子,这些簇产生的有机自由基中的电子,以及次级自由基中的电子。
这些金属中心参与了许多酶的反应。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('R David Britt', 18)}}的其他基金
Spectroscopy Investigations of Metalloenzyme Mechanisms
金属酶机理的光谱研究
- 批准号:
10378679 - 财政年份:2018
- 资助金额:
$ 31.59万 - 项目类别:
Spectroscopic Investigations of Metalloenzyme Mechanisms
金属酶机制的光谱研究
- 批准号:
10552244 - 财政年份:2018
- 资助金额:
$ 31.59万 - 项目类别:
Spectroscopy Investigations of Metalloenzyme Mechanisms
金属酶机理的光谱研究
- 批准号:
9903396 - 财政年份:2018
- 资助金额:
$ 31.59万 - 项目类别:
Spectroscopy Investigations of Metalloenzyme Mechanisms
金属酶机理的光谱研究
- 批准号:
10160922 - 财政年份:2018
- 资助金额:
$ 31.59万 - 项目类别:
Mechanisms of Radical SAM Enzymes Probed by EPR Spectroscopy
EPR 光谱探讨自由基 SAM 酶的作用机制
- 批准号:
8926453 - 财政年份:2014
- 资助金额:
$ 31.59万 - 项目类别:
Mechanisms of Radical SAM Enzymes Probed by EPR Spectroscopy
EPR 光谱探讨自由基 SAM 酶的作用机制
- 批准号:
9132280 - 财政年份:2014
- 资助金额:
$ 31.59万 - 项目类别:
Pulsed EPR Studies of Biological Manganese Clusters
生物锰簇的脉冲 EPR 研究
- 批准号:
8005178 - 财政年份:2010
- 资助金额:
$ 31.59万 - 项目类别:
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