Mechanisms of Radical SAM Enzymes Probed by EPR Spectroscopy
EPR 光谱探讨自由基 SAM 酶的作用机制
基本信息
- 批准号:8632910
- 负责人:
- 金额:$ 31.59万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-09-15 至 2018-08-31
- 项目状态:已结题
- 来源:
- 关键词:9-mercaptodethiobiotinActive SitesAffectAnabolismAntibioticsAnticodonArizonaBerlinBindingBiochemicalBiologicalBiologyBiotinChemicalsChemistryCleaved cellCodon NucleotidesComplexDNA Sequence RearrangementData SetDivalent CationsElectron Nuclear Double ResonanceElectron Spin Resonance SpectroscopyElectronicsElectronsEnvironmentEnzymesEquipmentEventExcisionFamilyFelis catusFundingGoalsGuanineHawaiiHealthHumanHydrogenHydrogenaseIonsIsotope LabelingKineticsLabelLaboratoriesLearningLigandsLightLyaseMagnetic ResonanceMagnetismMass Spectrum AnalysisMeasuresMetalsMethodsModelingModificationNatureNitrogenNucleoside QOxygenPathway interactionsPlayPositioning AttributePreparationPropertyProtein BiosynthesisProteinsProtonsRNARadioisotopesReactionRestS-AdenosylmethionineSamplingSequence AnalysisSideSiteSpectroscopy, Fourier Transform InfraredSpectrum AnalysisStructureSulfurTimeTransfer RNATyrosineUnited States National Institutes of HealthUniversitiesVitaminsWorkbasebiotin synthasechemical reactiondesigndesthiobiotindivalent metalelectronic structureenzyme activitygeometric structureimprovedinstrumentinterestiron hydrogenasepublic health relevanceresearch studytetrahydropterinthiophanevitamin biosynthesis
项目摘要
Project Summary
Nature uses highly reactive radicals to carry out a diverse set of biochemical functions, many of which are essen-
tial to maintaining proper human health. These potent biological radical reactions need to be carried out safely,
producing essential specific products, without dangerous side reactions occurring. A large number of such rad-
ical reactions are performed by the family of radical SAM enzymes, which use a [4Fe-4S] center with a bound
S-adenosylmethionine (SAM) molecule to generate a strongly oxidizing 5'-deoxyadenosyl radical which can in
turn drive a large number of difficult chemical reactions. We will target mechanistic aspects of several classes of
radical SAM enzymes. Biotin synthase is a radical SAM enzyme that catalyzes the final step in the biosynthesis
of the vitamin biotin. A set of Fe-S and radical SAM maturase enzymes are used to build the unique Fe-S center
of Fe-Fe hydrogenase, an enzyme which catalyzes the important reduction of protons to dihydrogen and vice
versa. And radical SAM enzymes are used to modify many bases in transfer RNA, improving codon-anticodon
recognition in order to make protein synthesis more reliable. We are specifically interested in a radical SAM
enzyme QueE that is essential for generating 7-deazapurines. This proposal describes a magnetic resonance
spectroscopic approach to study such diverse radical SAM enzymes. Specifically, we are using electron para-
magnetic resonance (EPR) spectroscopy, which can precisely measure the magnetic environment of unpaired
electrons in the radical SAM Fe-S clusters, in the organic radicals that these clusters generate, and in secondary
metal centers that are involved in the reactions in many of these enzymes.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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R David Britt其他文献
R David Britt的其他文献
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{{ truncateString('R David Britt', 18)}}的其他基金
Spectroscopy Investigations of Metalloenzyme Mechanisms
金属酶机理的光谱研究
- 批准号:
10378679 - 财政年份:2018
- 资助金额:
$ 31.59万 - 项目类别:
Spectroscopic Investigations of Metalloenzyme Mechanisms
金属酶机制的光谱研究
- 批准号:
10552244 - 财政年份:2018
- 资助金额:
$ 31.59万 - 项目类别:
Spectroscopy Investigations of Metalloenzyme Mechanisms
金属酶机理的光谱研究
- 批准号:
9903396 - 财政年份:2018
- 资助金额:
$ 31.59万 - 项目类别:
Spectroscopy Investigations of Metalloenzyme Mechanisms
金属酶机理的光谱研究
- 批准号:
10160922 - 财政年份:2018
- 资助金额:
$ 31.59万 - 项目类别:
Mechanisms of Radical SAM Enzymes Probed by EPR Spectroscopy
EPR 光谱探讨自由基 SAM 酶的作用机制
- 批准号:
8926453 - 财政年份:2014
- 资助金额:
$ 31.59万 - 项目类别:
Mechanisms of Radical SAM Enzymes Probed by EPR Spectroscopy
EPR 光谱探讨自由基 SAM 酶的作用机制
- 批准号:
9132280 - 财政年份:2014
- 资助金额:
$ 31.59万 - 项目类别:
Pulsed EPR Studies of Biological Manganese Clusters
生物锰簇的脉冲 EPR 研究
- 批准号:
8005178 - 财政年份:2010
- 资助金额:
$ 31.59万 - 项目类别:
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