Mass spectrometry imaging: Linking neurodegeneration with environmental exposure

描述（由申请人提供）：神经退行性疾病（ND），如：阿尔茨海默氏症，帕金森氏症和肌萎缩侧索硬化症与环境暴露于遗传风险因素背景下的许多化学物质有关。然而，环境因素的确切作用和疾病标志物的建立仍然难以捉摸；特别是在疾病发作之前出现的早期标记。对于帕金森病等ND疾病，氧化应激和线粒体功能障碍是已知的促成因素，大脑中对神经元功能至关重要的富含脂质区域是敏感靶点，在疾病病因学中起关键作用。局部脂质浓度

DESCRIPTION (provided by applicant): Neurodegenerative diseases (ND) such as: Alzheimer's, Parkinson's and Amyotrophic Lateral Sclerosis have been linked with environmental exposures to numerous chemicals within a background of genetic risk factors. However, the exact role of environmental agents and the establishment of markers of disease have remained elusive; particularly, early markers that occur prior to disease onset. For ND diseases such as Parkinson's, oxidative stress and mitochondrial dysfunction are known contributing factors and the lipid rich regions of the brain which are critical for neuronal functin are sensitive targets, playing a key role in disease etiology. Localized lipid concentrations offer important information on the functional state and molecular pathobiology of ND. Traditional molecular biology techniques, such as in situ hybridization and immunohistochemistry, are ideally suited for characterization of mRNA and protein abundances while mass spectrometry imaging (MSI) enables identification and spatial localization of individual lipids and metabolites in tissues thereby providing important complementary information. This project is focused on the development of a complementary mass spectrometry-based platform for quantitative imaging of lipids and metabolites in tissues with high spatial resolution and high sensitivity for obtaining molecular markers of the early disease stages at a cellular level. This capability is critical to advancing the molecular level understanding of the effect of environmental chemical exposure on neurodegeneration.