BD Biosciences Influx

 描述(申请人提供)：流式细胞术已经成为根据特定参数分离细胞群体的主要工具，因此被越来越多的生物医学科学家所使用。然而，这种方法技术复杂，设备昂贵，需要在用户群体之间共享仪器，以促进专家使用和获得这一重要和多功能的仪器。哥伦比亚翻译免疫学中心(CCTI)Flow Core已经成为哥伦比亚大学医学中心(CUMC)生物医学研究人员进行流动分类的“首选目的地”，因为可以使用高质量的操作员辅助分类。由于CCTI的增长(在3年内增加了7项PI)，以及非CCTI用户的需求增加，流动分拣器现在大幅超额认购。在NIH资助的研究中，6个主要用户需要人类细胞分选，通常是当天进行的，这些研究包括绘制组织中的T细胞群体图(Farber)，调查人类移植中的B细胞群体(Zorn)，临床研究(Clynes)，树突状细胞的人源化小鼠模型调查，癌症，自身免疫和移植(Liu，Sykes，Yang)，以及确定自身免疫T细胞的表观遗传学变化(Clynes)。炎症是许多人类疾病的核心，11个小型用户项目从移植、人类皮肤干细胞和自身免疫性斑秃到动脉粥样硬化，再到神经退行性疾病，不一而足。特别是在CUMC，人类免疫学的发展和对“随叫随到分类”的需求要求第二个CCTI分类器。我们借此机会说服医学部为加强人类细胞分选的BSL2+生物安全提供专门的空间。由于当前CCTI BD流入的大量使用，以及更高水平的生物安全遏制的好处，以及医学部坚定的机构承诺(BSL2+分拣室翻新和专门操作员的工资支持)，我们请求NIH提供资金，从BD Biosciences购买流入的分拣机。

 DESCRIPTION (provided by applicant): Flow cytometry has become the primary tool for the isolation of cell populations according to specific parameters, and is therefore employed by an ever-growing number of biomedical scientists. However, this method is technologically complex and the equipment expensive, necessitating shared instrumentation amongst groups of users to facilitate expert use and availability of this vital and versatile instrument. The Columbia Center for Translational Immunology (CCTI) Flow Core has become the "go-to destination" for flow sorting by biomedical researchers at Columbia University Medical Center (CUMC) because of the available of high-quality operator assisted sorts. As a result of the growth of the CCTI (7 additional PIs in 3 years) and the increased demand from non-CCTI users, the flow sorter is now substantially oversubscribed. The 6 Major Users require human cell sorting, often same-day, for their NIH-funded studies, which include, mapping T cell populations in tissues (Farber), investigating B cell populations in human transplant (Zorn), clinical studies (Clynes), investigations of humanized mouse models of dendritic cells, cancer, autoimmunity and transplantation (Liu, Sykes, Yang), and identification of epigenetic changes in autoimmune T cells (Clynes). Inflammation is at the heart of many human diseases and the eleven Minor User projects vary from transplantation, human skin stem cells and autoimmune alopecia areata, to atherosclerosis, to neurodegenerative disease. The growth in human immunology at CUMC and the need for "on-call sorting", in particular, mandates a second CCTI sorter. We have taken this opportunity to convince the Department of Medicine to provide dedicated space for enhanced BSL2+ Biosafety for human cell sorting. Due to the intensive usage of the current CCTI BD Influx and the benefit of higher level of biosafety containment, and with solid institutional commitment of the Department of Medicine (BSL2+ sorter room renovation and salary support for a dedicated operator) we request NIH funding for the purchase of an Influx sorter from BD Biosciences.