CCR-Frederick Flow Cytometry Core

CCR-Frederick 流式细胞术核心

• 批准号: 9154326
• 负责人: Kathleen Noer
• 金额: $ 70.81万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9154326
• 关键词: Aplastic Anemia; Autoimmune Diseases; Basic Science; Cell Separation; Cells; Complex; Contractor; Data; Data Analyses; Erythroid; Erythropoiesis; Flow Cytometry; Fluorochrome; Future; Genetic Transcription; Goals; Government; Hematopoietic stem cells; Individual; Inflammation; Laboratories; Lasers; Lewis Lung Carcinoma; Malignant Neoplasms; NCI Center for Cancer Research; National Cancer Institute; Paper; Population; Productivity; Proteins; Publishing; Regulation; Regulatory T-Lymphocyte; Repression; Research; Research Personnel; Resources; Role; Running; Sampling; Science; Services; Sorting - Cell Movement; Staining method; Stains; Stem cells; TNFRSF5 gene; Technology; Time; Training; Transgenic Mice; Translations; Update; Work; adaptive immunity; anticancer research; base; design; detector; experience; instrument; instrumentation; interest; investigator training; macrophage; novel; prevent; progenitor; programs; repaired; research study; stem cell differentiation; tool; tumor

The CCR-Flow Core is an indispensable resource for NCI and contractor investigators at the Fredereick National Lab for Cancer research. ninety seven investigators from 30 different laboratories have used the expertise of the flow core staff and the instrumentation available and maintained by the staff. The Flow Cytometry Core staff and instrumentation have supported the following studies: sorting support for adaptive immunity of commensals using RAG transgenic mice with a tdtomato fluorescent protein, the role of MDSC's and regulatory T cells in anti-CD40 tumor therapy, the regulation of erythropoiesis of hematopoietec stem cells by Id2 and gfI-1; demonstrating the role of microbiota in autoimmune disease; showing the suppressive activity of CD4+Foxp3+ regulatory T cells by TNFalpha and the homeostatic effects of IKKalpha on these T regulatory cells; role of IFNgamma altering hematopoietic stem cell differentiation in aplastic anemia; studies on the crosstalk of macrophages in Lewis lung carcinoma and the erythroid transcription and Id2 repression of erythroid progenitors. The instruments are used daily by the trained investigators often into the night and on the weekends. The flow core has been training investigators outside of the CIP to analyze their own samples expanding the base of user/operators. This leaves more time for the flow core staff to sort approximately 424 samples and analyze about 8474 samples in the last 10 months of this year. Without the investigators acquiring the data and analyzing their own samples, the government would have to hire full time experienced individuals to perform the same volume of work. At least 13 papers have been published in the past year using data obtained in the flow core. The future goals include keeping up with cutting-edge technology in the flow lab by continuing to expand the pool of investigators trained to run and analyze their own samples to all of CCR in Frederick thereby expanding the use, productivity and quality of the science generated using the core's instrumentation and expertise. We have up-graded all of the instruments so that laser and fluorochrome capabilities are more interchangeable giving investigators the flexability to use more than one instrument. This would prevent loss of data because the instrument needed was in use by another investigator or down because of needed repairs. The cell sorters have also been up-graded to allow direct translation of an experiment developed on an analyzer to the cell sorter to sort out the populations of cells of interest to be further studied. It is critical to have this flexability in analysis of samples as well as for sorting of cells. We would also like to stay a cutting edge facility which would require at least one analyzer to be up-graded to analyze at least 28 parameters at once.

CCR-FLOW核心是NCI和Fredereick国家癌症研究实验室承包商调查人员不可或缺的资源。来自30个不同实验室的97名调查人员利用了FLOW核心工作人员的专门知识以及工作人员提供和维护的仪器。流式细胞仪核心工作人员和仪器支持了下列研究：利用带有td西红柿荧光蛋白的RAG转基因小鼠分选对共生体适应性免疫的支持；MDSC和调节T细胞在抗CD40肿瘤治疗中的作用；Id2和GFI-1对造血干细胞的红细胞生成的调节；展示微生物区系在自身免疫性疾病中的作用；显示TNFpha对CD4+Foxp3+调节T细胞的抑制活性和IKKalpha对这些T调节细胞的稳态作用；IFNGamma改变造血干细胞分化在再生障碍性贫血中的作用；研究AGEs在Lewis肺癌中的串连以及红系祖细胞的红系转录和Id2抑制。训练有素的调查人员每天都在使用这些仪器，通常是在深夜和周末。Flow核心一直在培训CIP以外的调查人员分析他们自己的样本，扩大用户/运营商的基础。这使得Flow核心工作人员有更多的时间在今年最后10个月对大约424个样本进行分类，并分析大约8474个样本。如果调查人员没有获取数据并分析自己的样本，政府将不得不聘请有经验的全职人员来完成同样数量的工作。在过去的一年里，至少发表了13篇论文，使用的是Flow Core获得的数据。未来的目标包括跟上流动实验室的尖端技术，继续扩大受过培训的研究人员库，以运行和分析他们自己的样品到弗雷德里克的所有CCR，从而扩大使用核心的仪器和专业知识产生的科学的用途、生产力和质量。我们对所有仪器进行了升级，使激光和荧光的能力更具互换性，使研究人员可以灵活地使用多种仪器。这将防止数据丢失，因为需要的仪器正在被另一名调查人员使用或因需要维修而停机。细胞分选器也已升级，允许将在分析仪上开发的实验直接转化为细胞分选器，以筛选出要进一步研究的感兴趣的细胞群体。在样品分析和细胞分选中具有这种灵活性是至关重要的。我们还希望保留一个尖端设备，这将需要至少一个分析仪升级，以便一次分析至少28个参数。