CCR-Frederick Flow Cytometry Core

CCR-Frederick 流式细胞术核心

• 批准号: 8938472
• 负责人: Kathleen Noer
• 金额: $ 86.91万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8938472
• 关键词: Aplastic Anemia; Bacteria; Basic Science; Cell Separation; Cells; Colitis; Complex; Contractor; Data; Data Analyses; Erythropoiesis; Flow Cytometry; Fluorochrome; Future; Goals; Government; Hematopoiesis; Immune; In Vitro; Individual; Inflammation; Interferon Type II; Laboratories; Lasers; Malignant Neoplasms; Malignant neoplasm of lung; Mus; NCI Center for Cancer Research; National Cancer Institute; Oral; Paper; Population; Predisposition; Productivity; Protein Family; Proteins; Publishing; Recombinants; Regulation; Regulatory T-Lymphocyte; Research; Research Personnel; Resources; Role; Running; Sampling; Science; Services; Silicosis; Sorting - Cell Movement; Staining method; Stains; Stem cells; TNFRSF5 gene; Technology; Time; Training; Transgenic Mice; Translations; Update; Work; Zinc Fingers; adaptive immunity; anticancer research; attenuation; base; design; detector; experience; in vivo; instrument; instrumentation; interest; investigator training; novel; prevent; programs; repaired; research study; stem; tool; tumor

The CCR-Flow Core is an indispensable resource for NCI and contractor investigators at the Fredereick National Lab for Cancer research. Seventy six investigators from 37 different laboratories have used the expertise of the flow core staff and the instrumentation available and maintained by the staff. The Flow Cytometry Core staff and instrumentation have supported the following studies: sorting support for adaptive immunity of commensals using RAG transgenic mice with a tdtomato fluorescent protein, the role of MDSC's and regulatory T cells in anti-CD40 tumor therapy, the regulation of erythropoiesis of hematopoitec stem cells by Id2 and the zinc finger protein Pogz; demonstrating the attenuation of immune colitis in mice with an oral delivery of IL-27 recombinant bacteria; showing that in vitro generated Th17 cells can support the expansion and phenotypic stability of CD4+Foxp3+ regulatory T cells in vivo; studies of suppressive oligodeoxynucleotides that reduce lung cancer susceptibility in mice with silicosis; studies of how IFN-gamma causes aplastic anemia by altering hematopoiesis stem/progenitor cell composition and disrupting lineage differentiation; several studies on different aspects of T regulatory cells and several studies on different aspects of the KIR family of proteins. The instruments are used daily by the trained investigators often into the night and on the weekends. The flow core has been training investigators outside of the CIP to analyze their own samples expanding the base of user/operators.This leaves more time for the flow core staff to sort approximately 371 samples and analyze about 7426 samples in the first three quarters of this year. Without the investigators acquiring the data and analyzing their own samples, the government would have to hire full time experienced individuals to perform the same volume of work. At least 15 papers have been published, 1 paper is in press and 14 papers have been submitted in the past year using data obtained in the flow core. The future goals include keeping up with cutting-edge technology in the flow lab by continuing to expand the pool of investigators trained to run and analyze their own samples to all of CCR in Frederick thereby expanding the use, productivity and quality of the science generated using the core's instrumentation and expertise. We have approval to up-grade all of the instruments so that laser and fluorochrome capabilities are more interchangeable giving investigators the flexability to use more than one instrument. This would prevent loss of data because the instrument needed was in use by another investigator or down because of needed repairs. The cell sorters will also be up-graded to allow direct translation of an experiment developed on an analyzer to the cell sorter to sort out the populations of cells of interest to be further studied. It is critical to have this flexability in analysis of samples as well as for sorting of cells. We would also like to stay a cutting edge facility which would require at least one analyzer to be up-graded to analyze at least 28 parameters at once.

CCR-FLOW核心是NCI和Fredereick国家癌症研究实验室承包商调查人员不可或缺的资源。来自37个不同实验室的76名调查人员利用了FLOW核心工作人员的专业知识以及工作人员提供和维护的仪器。流式细胞仪的核心工作人员和仪器支持以下研究：利用带有td西红柿荧光蛋白的RAG转基因小鼠分选对共生体适应性免疫的支持，MDSC和调节性T细胞在抗CD40肿瘤治疗中的作用，Id2和锌指蛋白Pogz调节造血干细胞的红细胞生成；展示口服IL-27重组细菌对小鼠免疫性结肠炎的缓解；表明体外产生的Th17细胞可以支持体内CD4+Foxp3+调节性T细胞的扩增和表型稳定性；抑制寡核苷酸降低矽肺小鼠肺癌易感性的研究；关于干扰素-γ如何通过改变造血干/祖细胞组成和干扰谱系分化而导致再生障碍性贫血的研究；关于T调节细胞不同方面的几项研究；以及关于KIR蛋白家族不同方面的几项研究。训练有素的调查人员每天都在使用这些仪器，通常是在深夜和周末。Flow Core一直在培训CIP以外的调查人员分析他们自己的样本，扩大了用户/操作员的基础，这使Flow核心工作人员有更多时间在今年头三季度对大约371个样本进行分类和分析约7426个样本。如果调查人员没有获取数据并分析自己的样本，政府将不得不聘请有经验的全职人员来完成同样数量的工作。在过去的一年里，至少发表了15篇论文，1篇正在出版，14篇论文利用流动核心中获得的数据提交。未来的目标包括跟上流动实验室的尖端技术，继续扩大受过培训的研究人员库，以运行和分析他们自己的样品到弗雷德里克的所有CCR，从而扩大使用核心的仪器和专业知识产生的科学的用途、生产力和质量。我们已批准对所有仪器进行升级，以便激光和荧光性能更具互换性，使研究人员可以灵活地使用多台仪器。这将防止数据丢失，因为需要的仪器正在被另一名调查人员使用或因需要维修而停机。细胞分选器也将升级，以允许将在分析仪上开发的实验直接转化为细胞分选器，以筛选出需要进一步研究的感兴趣的细胞群体。在样品分析和细胞分选中具有这种灵活性是至关重要的。我们还希望保留一个尖端设备，这将需要至少一个分析仪升级，以便一次分析至少28个参数。