CCR-Frederick Flow Cytometry Core

CCR-Frederick 流式细胞术核心

• 批准号: 8554117
• 负责人: Kathleen Noer
• 金额: $ 92万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8554117
• 关键词: Accounting; Basic Science; Biology; Bone Marrow Stem Cell; Cells; Color; Complex; Contractor; Data; Data Analyses; Flow Cytometry; Future; Goals; Government; Human; Human T-lymphotropic virus 1; Individual; Infection; Inflammation; Inflammatory Response; Interleukins; Laboratories; Laboratory Scientists; Lasers; Leukocytes; Malignant Neoplasms; Mus; NCI Center for Cancer Research; National Cancer Institute; Natural Killer Cells; Paper; Population; Principal Investigator; Productivity; Publishing; Research; Research Personnel; Resources; Role; Running; Sampling; Science; Services; Sorting - Cell Movement; Staining method; Stains; Technology; Time; Training; Tumor Biology; Tumor Necrosis Factor-alpha; Tumor Stem Cells; Update; Work; anticancer research; design; detector; experience; instrument; instrumentation; investigator training; novel; programs; research study; tool

The CCR-Flow Core is an indispensable resource for NCI and contractor investigators at the Frdereick National Lab for Cancer research. Investigators have used the expertise of the flow core staff and the instrumentation available and maintained by the staff to investigate the role of various interleukins and tumor necrosis factor in tumor biology and the role of the inflammatory response in tumorogenesis. Another lab is investigating the basic biology of tumor stem cells. Other labs are investigating mechanism of differentiation of mouse bone marrow stem cells. Other labs are investigating the role of natural killer cells in the recognition of human leukocyte anigen B and the role of kir3DS1 in HTLV-1 infection.The flow core staff has trained eighteen investigators in the first three quarters of the year to run their own samples. The instruments are used daily by the trained investigators often into the night and on the weekends. This leaves more time for the flow core staff to sort approximately 550 samples and analyze about 7700 samples in the first three quarters of this year. Without the investigators acquiring the data and analyzing their own samples, the government would have to hire full time experienced individuals to perform the same volume of work. At least 19 papers have been published in the past year using data obtained in the flow core. In the past year, data has been generated (or samples have been sorted) for 79 individual scientists from the laboratories of 41 principal investigators. Although the CCR-Frederick Flow Cytometry Core is part of the CIP, investigators from this program account for approximately 27% of the use of this facility.The future goals include keeping up with cutting-edge technology in the flow lab by expanding the pool of investigators trained to run and analyze their own samples to all of CCR in Frederick thereby expanding the use, productivity and quality of the science generated using the core's instrumentation and expertise. This could be achieved by adding a 405 laser to the LSRII Fortessa and replacing the two BD FACScans with small 2 laser 6-color instruments.

CCR-Flow核心是NCI和弗雷德里克国家癌症研究实验室的承包商研究人员不可或缺的资源。研究人员利用流动核心工作人员的专业知识和工作人员提供的仪器来研究各种白细胞介素和肿瘤坏死因子在肿瘤生物学中的作用以及炎症反应在肿瘤发生中的作用。另一个实验室正在研究肿瘤干细胞的基本生物学。其他实验室正在研究小鼠骨髓干细胞分化的机制。其他实验室正在研究自然杀伤细胞在识别人类白细胞抗原B中的作用，以及kir3DS1在HTLV-1感染中的作用。流动核心人员在今年前三个季度培训了18名调查人员来运行他们自己的样本。这些仪器每天都由训练有素的调查人员使用，经常使用到深夜和周末。这使得流量核心人员有更多的时间在今年前三个季度对大约550个样品进行分类，并分析大约7700个样品。如果没有调查人员获取数据并分析他们自己的样本，政府将不得不聘请有经验的全职人员来完成同样数量的工作。在过去的一年里，至少有19篇论文使用了从流动岩心中获得的数据。在过去的一年里，已经为来自41个主要研究人员的实验室的79名科学家生成了数据（或整理了样本）。虽然CCR-Frederick流式细胞仪核心是CIP的一部分，但来自该项目的研究人员约占该设备使用的27%。未来的目标包括通过扩大经过培训的研究人员的数量，以运行和分析弗雷德里克所有CCR的自己的样品，从而扩大使用核心仪器和专业知识产生的科学的使用，生产力和质量，从而跟上流动实验室的尖端技术。这可以通过在LSRII Fortessa上增加一台405激光器和用小型2激光6色仪器替换两台BD facscan来实现。