Rapid Biomonitoring of Volatile Toxins in Human Samples (BIO-VOTO)

 描述（由申请人提供）：生物监测测量一个人的生物样本，如血液或尿液中的化学物质及其代谢物。它表示人类通过呼吸、触摸和摄入的化学品暴露量，这些来源包括但不限于空气、土壤、水、灰尘和食物。 通过国家生物监测计划（NBP）和国家健康和营养检查调查，疾病预防控制中心已经建立了金标准方法来测量血液，血清，尿液，母乳和胎粪中的450多种化学物质和营养标志物。然而，需要一种便携式多功能系统来在样本采集时分析来自类似范围样本的多种分析物，以显着提高检测准确性。此外，还需要采用其他成本较低的方法进行大规模人群（流行病学）研究，以验证现行标准，并在必要时制定新的接触准则。 我们设计了一个集成的仪器来检测挥发性毒素在人体样本中的快速生物监测，可以测量挥发性有机化合物（VOCs）的浓度与便携式，低成本，可部署的设备，具有类似于台式GC-MS（金标准）系统的灵敏度和特异性的生物样品。在初步研究中，我们已经证明了该设备的新概念及其准确测量人体血浆中挥发性有机物的能力。成功完成的项目里程碑是证明器械的统计学显著相关性 在双盲研究中，用GC-MS的标准测量读数。为了实现这一目标，我们将建立在我们的原型设备上，该设备在快速检测血浆中的有机化合物方面表现出显着的灵敏度，通过实施软件和硬件变化，这些变化将导致灵敏度和特异性，从而能够测量VOCs的痕量生物监测。这项工作的成功完成将使我们能够对拟议的设备进行更严格的测试，最终用于各种人体样本中的VOC和其他毒素的生物监测。

 DESCRIPTION (provided by applicant): Biomonitoring measures chemicals and their metabolites in a person's biological samples, such as blood or urine. It indicates the amount of exposure to chemicals by humans from breathing, touching and ingestion combined from sources including but not limited to air, soil, water, dust, and food. Through the National Biomonitoring Program (NBP) and The National Health and Nutrition Examination Survey, the CDC has established gold standard methods to measure over 450 chemicals and nutritional markers in blood, serum, urine, breast milk and meconium. However, a portable and versatile system is needed to analyze multiple analytes from similar range of samples at the point of sample collection to significantly increase detection accuracy. Additionally, alternative lower cos method is needed to allow large population (epidemiology) studies in order to verify current standards and, if necessary, formulate new guidelines for exposure. We designed an integrated instrument to detect volatile toxin in human samples for rapid biomonitoring that can measure Volatile Organic Compounds (VOCs) concentration in biological samples with a portable, low cost, deployable device, with the sensitivity and specificity similar to a bench top GC-MS (gold standard) system. In preliminary studies, we have demonstrated the novel concept of the device and its ability to accurately measure volatile organics in human plasma. The project milestone for successful completion is the demonstration of a statistically significant correlation of device readout with standard measures of GC-MS in a double-blind study. To achieve this goal, we will build upon our prototype device, which has shown significant sensitivity in rapidly detecting organic compounds in plasma, by implementing software, and hardware changes that would lead to sensitivities and specificities that would enable the measurement of trace biomonitor of VOCs. Successful completion of this work will allow us to proceed to more rigorous testing of the proposed device for ultimate use in biomonitor of VOCs and other toxins in a variety of human samples.