Single Cell Spatiotemporal and Functional Reporting using Magneto-Endosymbionts

使用磁内共生体进行单细胞时空和功能报告

• 批准号: 8832663
• 负责人: Caleb B. Bell
• 金额: $ 22.47万
• 依托单位: BELL BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-23 至 2015-12-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8832663
• 关键词: Achievement; Address; Bacteria; Brain; Cancer Model; Cell Therapy; Cell Transplants; Cells; Clinical; Collaborations; Computer Simulation; Computer software; Contrast Media; Detection; Disseminated Malignant Neoplasm; Effectiveness; Equilibrium; Escherichia coli; Event; Evolution; Generations; Genetic; Goals; Growth; Hour; Implant; In Situ; Iron; Label; Life; Location; Magnetic Resonance Imaging; Magnetism; Magnetospirillum; Malignant neoplasm of brain; Measures; Molecular Biology; Mus; Optical reporter; Organelles; Phase; Physiologic pulse; Protocols documentation; Regenerative Medicine; Relative (related person); Reporter; Reporting; Research; Research Personnel; Resolution; Scanning; Small Business Innovation Research Grant; System; Techniques; Technology; Time; Universities; Work; base; cancer cell; design and construction; endosymbiont; in vivo; insight; iron oxide; oncology; particle; personalized diagnostics; pre-clinical research; programs; public health relevance; radio frequency; research study; spatiotemporal; success; theories; tool

DESCRIPTION (provided by applicant): The goal of this proposal is to further develop our magneto-endosymbiont (ME) technology for in vivo single cell MRI detection (Phase 1) and functional reporting (Phase 2) thus creating a robust translational tool for cell based research. MEs were derived from magnetic bacteria creating a living contrast agent that enables in vivo cell tracking. MRI is a highly translatable technique for visualizing implanted cells because it is non- invasive and provides extremely high anatomical resolution. MEs are a unique contrast agent because they have a large genetic capacity that can be leveraged to encode functional reporters, in vivo. The effectiveness of MEs for single cell tracking will be experimentally assessed in a metastatic brain cancer model, that we have previously used to demonstrate single cell MRI tracking with superparamagnetic iron oxide particles. Tools that provide insight into detailed cellular events at the single cell level in situ will advance oncology research, regenerative medicine research, personalized diagnostics and the clinical realization of cell therapies.

描述（由申请人提供）：本提案的目标是进一步开发我们的磁内共生体（ME）技术，用于体内单细胞MRI检测（第1阶段）和功能报告（第2阶段），从而为基于细胞的研究创建一个强大的翻译工具。ME源自磁性细菌，产生一种能够进行体内细胞追踪的活体造影剂。MRI是一种用于可视化植入细胞的高度可翻译的技术，因为它 非侵入性并提供极高的解剖分辨率。ME是一种独特的造影剂，因为它们具有大的遗传能力，可以在体内利用其编码功能性报告基因。将在转移性脑癌模型中对ME用于单细胞跟踪的有效性进行实验评估，我们先前已使用该模型来证明使用超顺磁性氧化铁颗粒的单细胞MRI跟踪。在单细胞水平上原位提供详细细胞事件的工具将推进肿瘤学研究，再生医学研究，个性化诊断和细胞疗法的临床实现。